Chemically sensitive 3D imaging at sub 100 nm spatial resolution using tomography in a scanning transmission x-ray microscope

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 6318, Issue , 2006, Pages

  Johansson, Göran A ^a   Dynes, James J ^a   Hitchcock, Adam P ^a   Tyliszczak, Tolek ^b   Swerhone, George D W ^c   Lawrence, John R ^c  

^a MCMASTER UNIVERSITY   (Canada)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c NWRI ^*  (Canada)

Author keywords

3D chemical imaging; Biofilms, spectroscopy; Scanning transmission x ray microscopy; Tomography

Indexed keywords

BIOFILMS; COMPUTERIZED TOMOGRAPHY; OPTICAL RESOLVING POWER; SCANNING TUNNELING MICROSCOPY; SPECTROSCOPIC ANALYSIS; X RAY MICROSCOPES;

3D CHEMICAL IMAGING; MULTIPLE ENERGY IMAGES; SCANNING TRANSMISSION X-RAY MICROSCOPY;

IMAGING SYSTEMS;

EID: 33750699993     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.681217     Document Type: Conference Paper

References (19)

1. Y. Wang, C. Jacobsen, J. Maser, A. Osanna, "Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II. Tomograpy" J. Microscopy 197, 80-93 (2000).
2. D. Weiß, G. Schneider, B. Niemann, P. Guttmann, D. Rudolph, G. Schmahl, "Computed tomography of cryogenic biological specimens based on X-ray microscopic images" Ultramicroscopy 84, 185-197 (2000).
3. C.A. Larabell and M.A. Le Gros, "X-ray Tomography Generates 3-D Reconstructions of the Yeast, Saccharomyces cerevisiae, at 60-nm Resolution", Molecular Biology of the Cell. 15, 957-962 (2004).
4. H. Ade, "X-ray Spectromicroscopy". In Vacuum Ultraviolet Spectroscopy II; Samson, J. A. R., Ederer, D. L., Eds.; Vol. 32 in Experimental Methods In The Physical Sciences series; Academic Press: New York, 225-261 (1998).
5. H. Ade and S. G. Urquhart, "NEXAFS spectroscopy and microscopy of natural and synthetic polymers". In Chemical Applications of Synchrotron Radiation; Sham, T. K., Ed.; World Scientific Publishing: Singapore, 285-355 (2002).
6. A. P. Hitchcock, C. Morin, X. Zhang, T. Araki, J.J. Dynes, H. Stöver, J.L. Brash, J.R. Lawrence and G.G. Leppard, "Soft X-ray Spectromicroscopy of Biological and Synthetic Polymer Systems", J. El. Spect. Rel. Phen. 144-147 (2005).
7. J. R. Lawrence, G. D. W. Swerhone, G. G. Leppard, T. Araki, X. Zhang, M. M. West, A. P. Hitchcock, "Compositional mapping of the exopolymeric matrix of microbial biofilms", Appl. Environ. Microbiol. 69, 5543-5554 (2003).
8. J. J. Dynes, T. Tyliszczak, T Araki, J. R. Lawrence, G. D. W. Swerhone, G. G. Leppard, A. P. Hitchcock, "Quantitative mapping of metal species in bacterial biofilms using scanning transmission X-ray microscopy", Environmental Science and Technology 40, 1556-1565 (2006).
9. J. J. Dynes, J. R. Lawrence, D. R. Korber, G. D. W. Swerhone, G. G. Leppard, A. P. Hitchcock, "Quantitative mapping of chlorhexidine in diatoms in natural river biofilms", Sci. Total Env., 2006, in press
10. A. P. Hitchcock, T. Araki, H. Ikeura-Sekiguchi, N. Iwata, K. Tani, " 3d chemical mapping of toners by serial section scanning transmission X-ray microscopy", J. de Physique 104, 509-512 (2003).
11. A. L. D. Kilcoyne, T. Tyliszczak, W. F. Steele, S. Fakra, P. Hitchcock, K. Franck, E. Anderson, B. Harteneck, E. G. Rightor, G. E. Mitchell, A. P. Hitchcock, L. Yang, T. Warwick, H. Ade, "Interferometrically controlled Scanning Transmission Microscopes at the Advanced Light Source", J. Synchrotron Radiat. 10, 125-136 (2003).
12. T. Warwick, H. Ade, A. L. D. Kilcoyne, M. Kritscher, T. Tylisczcak, S. Fakra, A. P. Hitchcock, P. Hitchcock, H. A. Padmore, "A New Bend Magnet Beam Line for Scanning Transmission X-ray Microscopy at the Advanced Light Source", J. Synchrotron Radiat. 9, 254-257 (2002).
13. J. Stöhr, NEXAFS Spectroscopy, Springer Tracts in Surface Sciences; Springer: Berlin, Vol. 25. 1992.
14. C. Jacobsen, S. Wirick, G. Flynn, C. Zimba, "Soft X-ray spectroscopy from image sequences with sub-100 nm spatial resolution", J. Microscopy 197, 173-184 (2000).
15. W. Baumeister, R. Grimm, J. Walz, "Electron tomography of molecules and cells" Trends in Cell Biology 9, 81-85, (1999).
16. aXis2000 is written in Interactive Data Language (IDL). It is available free for noncommercial use from http://unicom.mcmaster.ca/aXis2000.html
17. J. R. Kremer, D.N. Mastronarde, J.R. McIntosh, "Computer visualization of three-dimensional image data using IMOD" J. Struct. Biol. 116, 71-76 (1996).
18. D. N. Mastronarde, "Dual-axis tomography: an approach with alignment methods that preserve resolution.", J. Struct. Biol. 120, 343-352 (1997).
19. K. Pecher, D. Mccubbery, E. Kneedler, J. Rothe, J. Bargar, G. Meigs, L. Cox, K. Nealson, B. Tonner, "Quantitative charge state analysis of manganese biominerals in aqueous suspension using Scanning Transmission X-ray Microscopy (STXM)". Geochim. Cosmochima Acta 67,1089-1098 (2003).