Homologous response regulators KvgA, KvhA and KvhR regulate the synthesis of capsular polysaccharide in Klebsiella pneumoniae CG43 in a coordinated manner

Journal of Biochemistry

Volumn 140, Issue 3, 2006, Pages 429-438

  Lin, Ching Ting ^a   Huang, Teng Yi ^a   Liang, Wan Chun ^a   Peng, Hwei Ling ^a  

^a NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

Author keywords

cps expression; Homologous response regulator; Klebsiella pneumoniae CG43; KvgA; KvhA; KvhR

Indexed keywords

BACTERIAL POLYSACCHARIDE; BACTERIAL PROTEIN; BETA GALACTOSIDASE; DNA FRAGMENT; POLYSACCHARIDE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM; CONTROLLED STUDY; ESCHERICHIA COLI; EXPERIMENTAL MODEL; GEL MOBILITY SHIFT ASSAY; GENE CLUSTER; GENE CONTROL; GENE DELETION; GENE MUTATION; KLEBSIELLA PNEUMONIAE; NONHUMAN; PHENOTYPE; PLASMID; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; TRANSCRIPTION REGULATION;

ANIMALS; BASE COMPOSITION; BASE SEQUENCE; CLUSTER ANALYSIS; DNA PRIMERS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FEMALE; GENE EXPRESSION REGULATION, BACTERIAL; KLEBSIELLA PNEUMONIAE; LETHAL DOSE 50; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYSACCHARIDES, BACTERIAL; PROMOTER REGIONS (GENETICS); RESPONSE ELEMENTS; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE;

EID: 33750686621     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj168     Document Type: Article

References (53)

1. Stock, A.M., Robinson, V.L., and Goudreau, P.N. (2000) Two-component signal transduction. Annu. Rev. Biochem. 69, 183-215
2. Mizuno, T. (1998) His-Asp phosphotransfer signal transduction. J. Biochem. 123, 555-563
3. West, A.H. and Stock, A.M. (2001) Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem. Sci. 26, 369-376
4. Fabret, C., Feher, V.A., and Hoch, J.A. (1999) Two-component signal transduction in Bacillus subtilis: How one organism sees its world. J. Bacteriol. 181, 1975-1983
5. Wosten, M.M. and Groisman, E.A. (1999) Molecular characterization of the PmrA regulon. J. Biol. Chem. 274, 27185-27190
6. Kato, A., Latifi, T., and Groisman, E.A. (2003) Closing the loop: the PmrA/PmrB two-component system negatively controls expression of its posttranscriptional activator PmrD. Proc. Natl. Acad. Sci. USA 100, 4706-4711
7. Chou, F.F. and Kou, H.K. (1996) Endogenous endophthalmitis associated with pyogenic hepatic abscess. J. Am. Coll. Surg. 182, 33-36
8. Han, S.H. (1995) Review of hepatic abscess from Klebsiella pneumoniae. An association with diabetes mellitus and septic endophthalmitis. West. J. Med. 162, 220-224
9. Lau, Y.J., Hu, B.S., Wu, W.L., Lin, Y.H., Chang, H.Y., and Shi, Z.Y. (2000) Identification of a major cluster of Klebsiella pneumoniae isolates from patients with liver abscess in Taiwan. J. Clin. Microbiol. 38, 412-414
10. Peng, H.L., Wang, P.Y., Wu, J.L., Chiu, C.T., and Chang, H.Y. (1991) Molecular epidemiology of Klebsiella pneumoniae. Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 24, 264-271
11. Wiener, J., Quinn, J.P., Bradford, P.A., Goering, R.V., Nathan, C., Bush, K., and Weinstein, R.A. (1999) Multiple antibiotic-resistant Klebsiella and Escherichia coli in nursing homes. JAMA 281, 517-523
12. Matsushita, M. and Janda, K.D. (2002) Histidine kinases as targets for new antimicrobial agents. Bioorg. Med. Chem. 10, 855-867
13. Chang, H.Y., Lee, J.H., Deng, W.L., Fu, T.F., and Peng, H.L. (1996) Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43. Microb. Pathog. 20, 255-261
14. Konig, J., Bock, A., Perraud, A.L., Fuchs, T.M., Beier, D., and Gross, R. (2002) Regulatory factors of Bordetella pertussis affecting virulence gene expression. J. Mol. Microbiol. Biotechnol. 4, 197-203
15. Lai, Y.C., Yang, S.L., Peng, H.L., and Chang, H.Y. (2000) Identification of genes present specifically in a virulent strain of Klebsiella pneumoniae. Infect. Immun. 68, 7149-7151
16. Lai, Y.C., Lin, G.T., Yang, S.L., Chang, H.Y., and Peng, H.L. (2003) Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43. FEMS Microbiol. Lett. 218, 121-126
17. Lowe, T.M. and Eddy, S.R. (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res, 25, 955-964
18. Lai, Y.C., Peng, H.L., and Chang, H.Y. (2001) Identification of genes induced in vivo during Klebsiella pneumoniae CG43 infection. Infect. Immun. 69, 7140-7145
19. Skorupski, K. and Taylor, R.K. (1996) Positive selection vectors for allelic exchange. Gene 169, 47-52
20. Lai, Y.C., Peng, H.L., and Chang, H.Y. (2003) RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level. J. Bacteriol. 185, 788-800
21. Whitfield, C. and Paiment, A. (2003) Biosynthesis and assembly of Group 1 capsular polysaccharides in Escherichia coli and related extracellular polysaccharides in other bacteria. Carbohydr. Res. 338, 2491-2502
22. Whitfield, C. and Roberts, I.S. (1999) Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol. Microbiol. 31, 1307-1319
23. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York
24. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
25. Domenico, P., Schwartz, S., and Cunha, B.A. (1989) Reduction of capsular polysaccharide production in Klebsiella pneumonias by sodium salicylate. Infect. Immun. 57, 3778-3782
26. Blumenkrantz, N. and Asboe-Hansen, G. (1973) New method for quantitative determination of uronic acids. Anal. Biochem. 54, 484-489
27. Reed, L.J. and Muench, H. (1938) A simple method of estimating the fifty percent endpoints. Am. J. Hyg. 27, 493-497
28. Chang, C. and Stewart, R.C. (1998) The two-component system. Regulation of diverse signaling pathways in prokaryotes and eukaryotes. Plant Physiol. 117, 723-731
29. Stock, J.B., Stock, A.M., and Mottonen, J.M. (1990) Signal transduction in bacteria. Nature 344, 395-400
30. Volz, K. (1993) Structural conservation in the CheY superfamily. Biochemistry 32, 11741-11753
31. Utsumi, R., Katayama, S., Ikeda, M., Igaki, S., Nakagawa, H., Miwa, A., Taniguchi, M., and Noda, M. (1992) Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12. Nucleic Acids Symp. Ser. 149-150
32. Roy, C.R. and Falkow, S. (1991) Identification of Bordetella pertussis regulatory sequences required for transcriptional activation of the fhaB gene and autoregulation of the bvgAS operon. J. Bacteriol. 173, 2385-2392
33. Cotter, P.A. and Jones, A.M. (2003) Phosphorelay control of virulence gene expression in Bordetella. Trends Microbiol. 11, 367-373
34. Nishino, K., Inazumi, Y., and Yamaguchi, A. (2003) Global analysis of genes regulated by EvgA of the two-component regulatory system in Escherichia coli. J. Bacteriol. 185, 2667-2672
35. Masuda, N. and Church, G.M. (2003) Regulatory network of acid resistance genes in Escherichia coli. Mol. Microbiol. 48, 699-712
36. Kato, A., Ohnishi, H., Yamamoto, K., Furuta, E., Tanabe, H., and Utsumi, R. (2000) Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12. Biosci. Biotechnol. Biochem. 64, 1203-1209
37. Masuda, N. and Church, G.M. (2002) Escherichia coli gene expression responsive to levels of the response regulator EvgA. J. Bacteriol. 184, 6225-6234
38. Nishino, K. and Yamaguchi, A. (2001) Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters. J. Bacteriol. 183, 1455-1458
39. Tanabe, H., Yamasaki, K., Katoh, A., Yoshioka, S., and Utsumi, R. (1998) Identification of the promoter region and the transcriptional regulatory sequence of the evgAS operon of Escherichia coli. Biosci. Biotechnol. Biochem. 62, 286-290
40. Zhou, L., Lei, X.H., Bochner, B.R., and Wanner, B.L. (2003) Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems. J. Bacteriol. 185, 4956-4972
41. Fang, C.T., Chuang, Y.P., Shun, C.T., Chang, S.C., and Wang, J.T. (2004) A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications. J. Exp. Med. 199, 697-705
42. Park, S.H., Eriksen, J., and Henrikden, S.D. (1967) Structure of the capsular polysaccharide of Klebsiella pneumoniae type 2 (B). Acta Pathil. Microbiol. Scand. 69, 431-436
43. Majdalani, N. and Gottesman, S. (2005) The Res Phosphorelay: A Complex Signal Transduction System. Annu. Rev. Microbiol
44. Kabha, K., Nissimov, L., Athamna, A., Keisari, Y., Parolis, H., Parolis, L.A., Grue, R.M., Schlepper-Schafer, J., Ezekowitz, A.R., Ohman, D.E. et al. (1995) Relationships among capsular structure, phagocytosis, and mouse virulence in Klebsiella pneumoniae. Infect. Immun. 63, 847-852
45. Highsmith, A.K. and Jarvis, W.R. (1985) Klebsiella pneumoniae: selected virulence factors that contribute to pathogenicity. Infect. Control. 6, 75-77
46. Held, T.K., Jendrike, N.R., Rukavina, T., Podschun, R., and Trautmann, M. (2000) Binding to and opsonophagocytic activity of O-antigen-specific monoclonal antibodies against encapsulated and nonencapsulated Klebsiella pneumoniae serotype O1 strains. Infect. Immun. 68, 2402-2409
47. Wehland, M. and Bernhard, F. (2000) The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria. J. Biol. Chem. 275, 7013-7020
48. Arakawa, Y., Wacharotayankun, R., Nagatsuka, T., Ito, H., Kato, N., and Ohta, M. (1995) Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J. Bacteriol. 177, 1788-1796
49. Mouslim, C., Latifi, T., and Groisman, E.A. (2003) Signal-dependent requirement for the co-activator protein RcsA in transcription of the RcsB-regulated ugd gene. J. Biol. Chem. 278, 50588-50595
50. Ochsner, U.A. and Vasil, M.L. (1996) Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes. Proc. Natl. Acad. Sci. USA 93, 4409-4414
51. Chen, Y.T., Chang, H.Y., Lu, C.L., and Peng, H.L. (2004) Evolutionary analysis of the two-component systems in Pseudomonas aeruginosa PAO1. J. Mol. Evol. 59, 725-737
52. Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids. Res. 22, 4673-4680
53. Kumar, S., Tamura, K., Jakobsen, I.E., and Nei, M. (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244-1245