RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level

Journal of Bacteriology

Volumn 185, Issue 3, 2003, Pages 788-800

  Lai, Yi Chyi ^a   Peng, Hwei Ling ^b   Chang, Hwan You ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE;

ARTICLE; AUTOREGULATION; BACTERIAL MEMBRANE; BIOSYNTHESIS; CARBOXY TERMINAL SEQUENCE; DNA BINDING MOTIF; GENE; GENE ACTIVATION; GENE CONTROL; GENE EXPRESSION REGULATION; GENE FUNCTION; K2 CPS GENE; KLEBSIELLA PNEUMONIAE; MOLECULAR STABILITY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; RMPA2 GENE; SEQUENCE HOMOLOGY; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

ANIMALS; BACTERIAL PROTEINS; FEMALE; GENE EXPRESSION REGULATION, BACTERIAL; HOMEOSTASIS; KLEBSIELLA PNEUMONIAE; MICE; MICE, INBRED BALB C; POLYSACCHARIDES, BACTERIAL; PROMOTER REGIONS (GENETICS); TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); KLEBSIELLA; KLEBSIELLA PNEUMONIAE; NEGIBACTERIA; PROKARYOTA;

EID: 0037310768     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.3.788-800.2003     Document Type: Article

References (37)

1. Arakawa, Y., M. Ohta, R. Wacharotayankun, M. Mori, N. Kido, H. Ito, T. Komatsu, T. Sugiyama, and N. Kato. 1991. Biosynthesis of Klebsielia K2 capsular polysaccharide in Escherichia coli HB101 requires the functions of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2). Infect. Immun. 59:2043-2050.
2. Arakawa, Y., R. Wacharotayankun, T. Nagatsuka, H. Ito, N. Kato, and M. Ohta. 1995. Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J. Bacteriol. 177:1788-1796.
3. Blumenkrantz, N., and G. Asboe-Hansen. 1973, New method for quantitative determination of uronic acids. Anal. Biochem. 54:484-489.
4. Brill, J. A., C. Quinlan-Walshe, and S. Gottesman. 1988. Fine-structure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12. J. Bacteriol. 170:2599-2611.
5. Chang, H. Y., J. H. Lee, W. L. Deng, T. F. Fu, and H. L. Peng. 1996. Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43. Microb. Pathog. 20:255-261.
6. Cryz, S. J., Jr., F. Furer, and R. Germanier. 1984. Experimental Klebsiella pneumoniae burn wound sepsis: role of capsular polysaccharide. Infect. Immun. 43:440-441.
7. Domenico, P., S. Schwartz, and B. A. Cunha. 1989. Reduction of capsular polysaccharide production in Klebsiella pneumoniae by sodium salicylate. Infect. Immun. 57:3778-3782.
8. Ebel, W., and J. E. Trempy. 1999. Escherichia coli RcsA, a positive activator of colanic acid capsular polysaccharide synthesis, functions to activate its own expression. J. Bacteriol. 181:577-584.
9. Gottesman, S., and V. Stout. 1991. Regulation of capsular polysaccharide synthesis in Escherichia coli K12. Mol. Microbiol. 5:1599-1606.
10. Henderson, I. R., P. Owen, and J. P. Nataro. 1999. Molecular switches - the ON and OFF of bacterial phase variation. Mol. Microbiol. 33:919-932.
11. Highsmith, A. K., and W. R. Jarvis. 1985. Klebsiella pneumoniae: selected virulence factors that contribute to pathogenicity. Infect. Control 6:75-77.
12. Jann, A., and K. Jann. 1990. Structure and Biosynthesis of the capsular antigens of Escherichia coli. Curr. Top. Microbiol. Immunol. 150:19-42.
13. Jayaratne, P., W. J. Keenleyside, P. R. MacLachlan, C. Dodgson, and C. Whitfield. 1993. Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides. J. Bacteriol. 175:5384-5394.
14. Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70:191-197.
15. Lai, Y. C., H. L. Peng, and H. Y. Chang. 2001. Identification of genes induced in vivo during Klebsiella pneumoniae CG43 infection. Infect. Immun. 69:7140-7145.
16. Li, J., L. Passaglia, I. Rombel, D. Yan, and S. Kustu. 1999. Mutations affecting motifs of unknown function in the central domain of nitrogen regulatory protein C. J. Bacteriol. 181:5443-5454.
17. Mizuta, K., M. Ohta, M. Mori, T. Hasegawa, I. Nakashima, and N. Kato. 1983. Virulence for mice of Klebsiella strains belonging to the O1 group: relationship to their capsular (K) types. Infect. Immun. 40:56-61.
18. Mori, M., M. Ohta, N. Agata, N. Kido, Y. Arakawa, H. Ito, T. Komatsu, and N. Kato. 1989. Identification of species and capsular types of Klebsiella clinical isolates, with special reference to Klebsiella planticola. Microbiol. Immunol. 33:887-895.
19. Nassif, X., N. Honore, T. Vasselon, S. T. Cole, and P. J. Sansonetti. 1989. Positive control of colanic acid synthesis in Escherichia coli by rmpA and rmpB, two virulence-plasmid genes of Klebsiella pneumoniae. Mol. Microbiol. 3:1349-1359.
20. Nassif, X., and P. J. Sansonetti. 1986. Correlation of the virulence of Klebsiella pneumoniae K1 and K2 with the presence of a plasmid encoding aerobactin. Infect. Immun. 54:603-608.
21. Park, S. H., J. Eriksen, and S. D. Henriksen. 1967. Structure of the capsular polysaccharide of Klebsiella pneumoniae type 2 (B). Acta Pathol. Microbiol. Scand. 69:431-436.
22. Peng, H. L., P. Y. Wang, J. L. Wu, C. T. Chiu, and H. Y. Chang. 1991. Molecular epidemiology of Klebsiella pneumoniae. Clin. J. Microbiol. Immunol. (Taipei) 4:264-271.
23. Rahn, A., J. Drummelsmith, and C. Whitfield. 1999. Conserved organization in the cps gene clusters for expression of Escherichia coli group 1 K antigens: relationship to the colanic acid biosynthesis locus and the cps genes from Klebsiella pneumoniae. J. Bacteriol. 181:2307-2313.
24. Reed, L. J., and H. Muench. 1938. A simple method of estimating the fifty percent endpoints. Am. J. Hyg. 27:493-497.
25. Richards, H., and N. Datta. 1982. Plasmids and transposons acquired by Salmonella typhi in man. Plasmid 8:9-14.
26. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor laboratory, Cold Spring Harbor, N.Y.
27. Silhavy, T. J., M. L. Berman, and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Skorupski, K., and R. K. Taylor. 1996. Positive selection vectors for allelic exchange. Gene 169:47-52.
29. Stout, V., A. Torres-Cabassa, M. R. Maurizi, D. Gutnick, and S. Gottesman. 1991. RcsA, an unstable positive regulator of capsular polysaccharide synthesis. J. Bacteriol. 173:1738-1747.
30. Suerbaum, S., S. Friedrich, H. Leying, and W. Opferkuch. 1994. Expression of capsular polysaccharide determines serum resistance in Escherichia coli K92. Zentbl. Bakteriol. 281:146-157.
31. Trisler, P., and S. Gottesman. 1984. lon transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Eschenchia coli K-12. J. Bacteriol. 160:184-191.
32. Trun, N. J., and T. J. Silhavy. 1989. PrlC, a suppressor of signal sequence mutations in Escherichia coli, can direct the insertion of the signal sequence into the membrane. J. Mol. Biol. 205:665-676.
33. Wacharotayankun, R., Y. Arakawa, M. Ohta, T. Hasegawa, M. Mori, T. Horii, and N. Kato. 1992. Involvement of rcsB in Klebsiella K2 capsule synthesis in Escherichia coli K-12. J. Bacteriol. 174:1063-1067.
34. Wacharotayankun, R., Y. Arakawa, M. Ohta, K. Tanaka, T. Akashi, M. Mori, and N. Kato. 1993. Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ. Infect. Immun. 61:3164-3174.
35. Wehland, M., and F. Bernhard. 2000. The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria. J. Biol. Chem. 275:7013-7020.
36. Whitfield, C., and I. S. Roberts. 1999. Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol. Microbiol. 31:1307-1319.
37. Williams, P., P. A. Lambert, M. R. Brown, and R. J. Jones. 1983. The role of the O and K antigens in determining the resistance of Klebsiella aerogenes to serum killing and phagocytosis. J. Gen. Microbiol. 129:2181-2191.