Positive charges at the intracellular mouth of the pore regulate anion conduction in the CFTR chloride channel

Journal of General Physiology

Volumn 128, Issue 5, 2006, Pages 535-545

  St Aubin, Chantal N ^a,b   Linsdell, Paul ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ANION; ARGININE DERIVATIVE; CHLORIDE; CHLORIDE CHANNEL; CYSTEINE; ION; MUTANT PROTEIN; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; CYTOPLASM; ELECTRIC POTENTIAL; GENETIC REGULATION; MEMBRANE POTENTIAL; MUTAGENESIS; NONHUMAN; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; SURFACE CHARGE;

ANIMALS; ANIONS; ARGININE; CATIONS; CELL MEMBRANE; CHLORIDES; CRICETINAE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; HUMANS; ION CHANNEL GATING; KIDNEY; MUTAGENESIS, SITE-DIRECTED; MUTATION; PATCH-CLAMP TECHNIQUES;

EID: 33750530196     PISSN: 00221295     EISSN: 00221295     Source Type: Journal    
DOI: 10.1085/jgp.200609516     Document Type: Article

References (32)

1. Anderson, M.P., R.J. Gregory, S. Thompson, D.W. Souza, S. Paul, R.C. Mulligan, A.E. Smith, and M.J. Welsh. 1991. Demonstration that CFTR is a chloride channel by alteration of its anion selectivity. Science. 253:202-205.
2. Brelidze, T.I., X. Niu, and K.L. Magleby. 2003. A ring of eight conserved negatively charged amino acids doubles the conductance of BK channels and prevents inward rectification. Proc. Natl. Acad. Sci. USA. 100:9017-9022.
3. - channel. J. Gen. Physiol. 122:605-620.
4. Chen, M.-F., and T.-Y. Chen. 2003. Side-chain charge effects and conductance determinants in the pore of ClC-0 chloride channels. J. Gen. Physiol. 122:133-145.
5. Cheung, M., and M.H. Akabas. 1997. Locating the anion-selectivity filter of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. J. Gen. Physiol. 109:289-299.
6. Cotten, J.F., and M.J. Welsh. 1999. Cystic fibrosis-associated mutations at arginine 347 alter the pore architecture of CFTR. Evidence for disruption of a salt bridge. J. Biol. Chem. 274:5429-5435.
7. D'Avanzo, N., H.C. Cho, I. Tolokh, R. Pekhletski, I. Tolokh, C. Gray, S. Goldman, and P. Backx. 2005. Conduction through the inward rectifier potassium channel, Kir2.1, is increased by negatively charged extracellular residues. J. Gen. Physiol. 125:493-503.
8. Dawson, D.C., S.S. Smith, and M.K. Mansoura. 1999. CFTR: mechanism of anion conduction. Physiol. Rev. 79:S47-S75.
9. Gallet, X., F. Festy, P. Ducarme, R. Brasseur, and A. Thomas-Soumarmon. 1998. Topological model of membrane domain of the cystic fibrosis transmembrane conductance regulator. J. Mol. Graph. Model. 16:72-82.
10. Ge, N., C.N. Muise, X. Gong, and P. Linsdell. 2004. Direct comparison of the functional roles played by different transmembrane regions in the cystic fibrosis transmembrane conductance regulator chloride channel pore. J. Biol. Chem. 279:55283-55289.
11. Gong, X., and P. Linsdell. 2003a. Mutation-induced blocker permeability and multiion block of the CFTR chloride channel pore. J. Gen. Physiol. 122:673-687.
12. Gong, X., and P. Linsdell. 2003b. Molecular determinants and role of an anion binding site in the external mouth of the CFTR chloride channel pore. J. Physiol. 549:387-397.
13. - channel pore. J. Physiol. 540:39-47.
14. Green, W.N., and O.S. Andersen. 1991. Surface charges and ion channel function. Annu. Rev. Physiol. 53:341-359.
15. Guinamard, R., and M.H. Akabas. 1999. Arg352 is a major determinant of charge selectivity in the cystic fibrosis transmembrane conductance regulator chloride channel. Biochemistry. 38:5528-5537.
16. + conduction by external surface charge effect. J. Gen. Physiol. 124:173-184.
17. Imoto, K., C. Busch, B. Sakmann, M. Mishina, T. Konno, J. Nakai, H. Bujo, Y. Mori, K. Fukuda, and S. Numa. 1988. Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance. Nature. 335:645-648.
18. Linsdell, P. 2005. Location of a common inhibitor binding site in the cytoplasmic vestibule of the cystic fibrosis transmembrane conductance regulator chloride channel pore. J. Biol. Chem. 280:8945-8950.
19. Linsdell, P. 2006. Mechanism of chloride permeation in the cystic fibrosis transmembrane conductance regulator chloride channel. Exp. Physiol. 91:123-129.
20. - channels expressed in a mammalian cell line and its regulation by a critical pore residue. J. Physiol. 496:687-693.
21. Linsdell, P., and J.W. Hanrahan. 1998. Adenosine triphosphate-dependent asymmetry of anion permeation in the cystic fibrosis transmembrane conductance regulator chloride channel. J. Gen. Physiol. 111:601-614.
22. + channel. Biochemistry. 28:8092-8099.
23. Mansoura, M.K., S.S. Smith, A.D. Choi, N.W. Richards, T.V. Strong, M.L. Drumm, F.S. Collins, and D.C. Dawson. 1998. Cystic fibrosis transmembrane conductance regulator (CFTR) anion binding as a probe of the pore. Biophys. J. 74:1320-1332.
24. McCarty, N.A. 2000. Permeation through the CFTR chloride channel. J. Exp. Biol. 203:1947-1962.
25. Moorhouse, A.J., A. Keramidas, A. Zaykin, P.R. Schofield, and P.H. Barry. 2002. Single channel analysis of conductance and rectification in cation-selective, mutant glycine receptor channels. J. Gen. Physiol. 119:411-425.
26. Nimigean, C.M., J.S. Chappie, and C. Miller. 2003. Electrostatic tuning of ion conductance in potassium channels. Biochemistry. 42:9263-9268.
27. Riordan, J.R., J.M. Rommens, B. Kerem, A. Alon, R. Rozmahel, Z. Grzelczak, J. Zielenski, S. Lok, N. Plasvik, J.-L. Chou, et al. 1989. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science. 245:1066-1073.
28. Seibert, F.S., P. Linsdell, T.W. Loo, J.W. Hanrahan, J.R. Riordan, and D.M. Clarke. 1996. Cytoplasmic loop three of cystic fibrosis transmembrane conductance regulator contributes to regulation of chloride channel activity. J. Biol. Chem. 271:27493-27499.
29. Smith, S.S., X. Liu, Z.-R. Zhang, F. Sun, T.E. Kriewall, N.A. McCarty, and D.C. Dawson. 2001. CFTR: covalent and noncovalent modification suggests a role for fixed charges in anion conduction. J. Gen. Physiol. 118:407-431.
30. Tabcharani, J.A., P. Linsdell, and J.W. Hanrahan. 1997. Halide permeation in wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels. J. Gen. Physiol. 110:341-354.
31. Wang, Y., L. Xu, D.A. Pasek, D. Gillespie, and G. Meissner. 2005. Probing the role of negatively charged amino acid residues in ion permeation of skeletal muscle ryanodine receptor. Biophys. J. 89:256-265.
32. Worley, J.F., R.J. French, and B.K. Krueger. 1986. Trimethyloxonium modification of single batrachotoxin-activated sodium channels in planar bilayers. Changes in unit conductance and in block by saxitoxin and calcium. J. Gen. Physiol. 87:327-349.