Tissue-engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF-β1 in platelet-rich plasma

Journal of Cellular Physiology

Volumn 209, Issue 3, 2006, Pages 744-754

  Chen, Wei Hong ^a   Lo, Wen Cheng ^a   Lee, Jie Jen ^b   Su, Ching Hua ^a   Lin, Che Tong ^a   Liu, Hen Yu ^a   Lin, Tsou Wen ^a   Lin, Wei Chao ^c   Huang, Te Yang ^b   Deng, Win Ping ^a  

^a TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^b MACKAY MEMORIAL HOSPITAL   (Taiwan)

^c Chianan University of Pharmacy and Science   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AGGRECAN; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN; GROWTH FACTOR; MESSENGER RNA; SMAD2 PROTEIN; SMAD3 PROTEIN; TRANSCRIPTION FACTOR SOX9; TRANSFORMING GROWTH FACTOR BETA1;

ADULT; AGED; AGING; ARTICLE; CELL ACTIVATION; CELL AGGREGATION; CELL CULTURE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL ISOLATION; CELL PROLIFERATION; CELL STIMULATION; CELL STRUCTURE; CHONDROGENESIS; COLLAGEN SCAFFOLD; CONTROLLED STUDY; CULTURE OPTIMIZATION; DOSE TIME EFFECT RELATION; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; GENE INDUCTION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INTERVERTEBRAL DISK; INTERVERTEBRAL DISK DEGENERATION; MESENCHYMAL STEM CELL; NUCLEUS PULPOSUS; PRIORITY JOURNAL; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; THERAPY EFFECT; THROMBOCYTE RICH PLASMA; TISSUE ENGINEERING; TISSUE REGENERATION; TISSUE STRUCTURE; UPREGULATION;

ADULT; AGED; ANIMALS; APOPTOSIS; BLOOD PLATELETS; CELL PROLIFERATION; CHONDROGENESIS; CULTURE MEDIA, CONDITIONED; EXTRACELLULAR MATRIX; HUMANS; INTERVERTEBRAL DISK; MIDDLE AGED; PLASMA; PROTEOGLYCANS; REGENERATION; RNA, MESSENGER; SMAD2 PROTEIN; SMAD3 PROTEIN; TISSUE ENGINEERING; TRANSFORMING GROWTH FACTOR BETA1;

EID: 33750529139     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcp.20765     Document Type: Article

References (53)

1. Alini M, Li W, Markovic P, Aebi M, Spiro RC, Roughley PJ. 2003. The potential and limitations of a cell-seeded collagen/hyaluronan scaffold to engineer an intervertebral disc-like matrix. Spine 28(5):446-454; discussion 453.
2. Antoniou J, Steffen T, Nelson F, Winterbottom N, Hollander AP, Poole RA, Aebi M, Alini M. 1996. The human lumbar intervertebral disc: Evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. J Clin Invest 98(4):996-1003.
3. Arpornmaeklong P, Kochel M, Depprich R, Kubler NR, Wurzler KK. 2004. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg 33(1):60-70.
4. Bell DM, Leung KK, Wheatley SC, Ng LJ, Zhou S, Ling KW, Sham MH, Koopman P, Tam PP, Cheah KS. 1997. SOX9 directly regulates the type-II collagen gene. Nat Genet 16(2):174-178.
5. Bibby SR, Jones DA, Lee RB, Yu J, Urban JPG. 2001. The pathophysiology of the intervertebral disc. Joint Bone Spine 68(6):537-542.
6. Biering-Sorensen F. 1982. Low back trouble in a general population of 30-, 40-, 50-, and 60-year-old men and women. Study design, representativeness and basic results. Dan Med Bull 29(6):289-299.
7. Buckwalter JA. 1995. Aging and degeneration of the human intervertebral disc. Spine 20(11):1307-1314.
8. Chen CW, Tsai YH, Deng WP, Shih SN, Fang CL, Burch JG, Chen WH, Lai WF. 2005a. Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells. J Orthop Res 23(2):446-453.
9. Chen WH, Lai WF, Deng WP, Yang WK, Lo WC, Wu CC, Yang DM, Lai MT, Lin CT, Lin TW, Yang CB. 2005b. Tissue engineered cartilage using human articular chondrocytes immortalized by HPV-16 E6 and E7 genes. J Biomed Mater Res A 76:512-520.
10. Cole TC, Burkhardt D, Frost L, Ghosh P. 1985. The proteoglycans of the canine intervertebral disc. Biochim Biophys Acta 839(2):127-138.
11. Furumatsu T, Tsuda M, Taniguchi N, Tajima Y, Asahara H. 2005. Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment. J Biol Chem 280(9):8343-8350.
12. Gaissmaier C, Fritz J, Krackhardt T, Flesch I, Aicher WK, Ashammakhi N. 2005. Effect of human platelet supernatant on proliferation and matrix synthesis of human articular chondrocytes in monolayer and three-dimensional alginate cultures. Biomaterials 26(14):1953-1960.
13. Gruber HE, Norton HJ, Hanley EN, Jr. 2000. Anti-apoptotic effects of IGF-1 and PDGF on human intervertebral disc cells in vitro. Spine 25(17):2153-2157.
14. Hung SC, Yang DM, Chang CF, Lin RJ, Wang JS, Low-Tone Ho L, Yang WK. 2004. Immortalization without neoplastic transformation of human mesenchymal stem cells by transduction with HPV16 E6/E7 genes. Int J Cancer 110(3):313-319.
15. Hunter CJ, Matyas JR, Duncan NA. 2003. The notochordal cell in the nucleus pulposus: A review in the context of tissue engineering. Tissue Eng 9(4):667-677.
16. Ichitnura K, Tsuji H, Matsui H, Makiyama N. 1991. Cell culture of the intervertebral disc of rats: Factors influencing culture, proteoglycan, collagen, and deoxyribonucleic acid synthesis. J Spinal Disord 4(4):428-436.
17. Jahnke MR, McDevitt CA. 1988. Proteoglycans of the human intervertebral disc. Electrophoretic heterogeneity of the aggregating proteoglycans of the nucleus pulposus. Biochem J 251(2):347-356.
18. Kanno T, Takahashi T, Tsujisawa T, Ariyoshi W, Nishihara T. 2005. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg 63(3):362-369.
19. Konttinen YT, Kemppinen P, Li TF, Waris E, Pihlajamaki H, Sorsa T, Takagi M, Santavirta S, Schultz GS, Humphreys-Beher MG. 1999. Transforming and epidermal growth factors in degenerated intervertebral discs. J Bone Joint Surg Br 81(6):1058-1063.
20. Kotani Y, Abumi K, Shikinami Y, Takada T, Kadoya K, Shimamoto N, Ito M, Kadosawa T, Fujinaga T, Kaneda K. 2002. Artificial intervertebral disc replacement using bioactive three-dimensional fabric: Design, development, and preliminary animal study. Spine 27(9):929-935; discussion 935-926.
21. Krajickova J, Polakova R, Smetana K, Vytasek R. 1995. Age-dependent changes in proteoglycan biosynthesis in human intervertebral discs. Folia Biol (Praha) 41(1):41-51.
22. Lai WF, Tang JR, Chen CT. 2003. Fibrication of a cartilage implant. US patent 0152556A1.
23. Landesberg R, Roy M, Glickman RS. 2000. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg 58(3):297-300; discussion 300-291.
24. Lefebvre V, Huang W, Harley VR, Goodfellow PN, de Crombrugghe B. 1997. SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene. Mol Cell Biol 17(4):2336-2346.
25. Lin AH, Luo J, Mondshein LH, ten Dijke P, Vivien D, Contag CH, Wyss-Coray T. 2005. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury. J Immunol 175(1):547-554.
26. Liu Y, Kalen A, Risto O, Wahlstrom O. 2002. Fibroblast proliferation due to exposure to a platelet concentrate in vitro is pH dependent. Wound Repair Regen 10(5):336-340.
27. Lucarelli E, Beccheroni A, Donati D, Sangiorgi L, Cenacchi A, Del Vento AM, Meotti C, Bertoja AZ, Giardino R, Fornasari PM, Mercuri M, Picci P. 2003. Platelet-derived growth factors enhance proliferation of human stromal stem cells. Biomaterials 24(18):3095-3100.
28. Matsunaga S, Nagano S, Onishi T, Morimoto N, Suzuki S, Komiya S. 2003. Age-related changes in expression of transforming growth factor-beta and receptors in cells of intervertebral discs. J Neurosurg Spine 98(1):63-67.
29. Melrose J, Smith S, Ghosh P. 2003. Assessment of the cellular heterogeneity of the ovine intervertebral disc: Comparison with synovial fibroblasts and articular chondrocytes. Eur Spine J 12(1):57-65.
30. Miller JA, Schmatz C, Schultz AB. 1988. Lumbar disc degeneration: Correlation with age, sex, and spine level in 600 autopsy specimens. Spine 13(2):173-178.
31. Miyazawa K, Shinozaki M, Hara T, Furuya T, Miyazono K. 2002. Two major Smad pathways in TGF-beta superfamily signalling. Genes Cells 7(12):1191-1204.
32. O'Driscoll SW, Fitzsimmons JS. 2001. The role of periosteum in cartilage repair. Clin Orthop Relat Res(391 Suppl):S190-S207.
33. Oegema TR, Jr. 2002. The role of disc cell heterogeneity in determining disc biochemistry: A speculation. Biochem Soc Trans 30(Pt 6):839-844.
34. Okuda S, Myoui A, Ariga K, Nakase T, Yonenobu K, Yoshikawa H. 2001. Mechanisms of age-related decline in insulin-like growth factor-I dependent proteoglycan synthesis in rat intervertebral disc cells. Spine 26(22):2421-2426.
35. Osada R, Ohshima H, Ishihara H, Yudoh K, Sakai K, Matsui H, Tsuji H. 1996. Autocrine/paracrine mechanism of insulin-like growth factor-1 secretion, and the effect of insulin-like growth factor-1 on proteoglycan synthesis in bovine intervertebral discs. J Orthop Res 14(5):690-699.
36. Pearce RH, Mathieson JM, Mort JS, Roughley PJ. 1989. Effect of age on the abundance and fragmentation of link protein of the human intervertebral disc. J Orthop Res 7(6):861-867.
37. Romashkova JA, Makarov SS. 1999. NF-kappaB is a target of AKT in anti-apoptotic PDGF signalling. Nature 401(6748):86-90.
38. Roughley PJ. 2004. Biology of intervertebral disc aging and degeneration: Involvement of the extracellular matrix. Spine 29(23):2691-2699.
39. Roughley PJ, Alini M, Antoniou J. 2002. The role of proteoglycans in aging, degeneration and repair of the intervertebral disc. Biochem Soc Trans 30(Pt 6):869-874.
40. Sato M, Kikuchi T, Asazuma T, Yamada H, Maeda H, Fujikawa K. 2001. Glycosaminoglycan accumulation in primary culture of rabbit intervertebral disc cells. Spine 26(24):2653-2660.
41. Seguin CA, Grynpas MD, Pilliar RM, Waldman SD, Kandel RA. 2004. Tissue engineered nucleus pulposus tissue formed on a porous calcium polyphosphate substrate. Spine 29(12):1299-1306; discussion 1306-1307.
42. Sekiya I, Tsuji K, Koopman P, Watanabe H, Yamada Y, Shinomiya K, Nifuji A, Noda M. 2000. SOX9 enhances aggrecan gene promoter/enhancer activity and is up-regulated by retinoic acid in a cartilage-derived cell line, TC6. J Biol Chem 275(15):10738-10744.
43. Stern S, Lindenhayn K, Schultz O, Perka C. 2000. Cultivation of porcine cells from the nucleus pulposus in a fibrin/hyaluronic acid matrix. Acta Orthop Scand 71(5):496-502.
44. Sun Y, Hurtig M, Pilliar RM, Grynpas M, Kandel RA. 2001. Characterization of nucleus pulposus-like tissue formed in vitro. J Orthop Res 19(6):1078-1084.
45. Sztrolovics R, Alini M, Roughley PJ, Mort JS. 1997. Aggrecan degradation in human intervertebral disc and articular cartilage. Biochem J 326(Pt 1):235-241.
46. Thompson JP, Oegema TR, Jr., Bradford DS. 1991. Stimulation of mature canine intervertebral disc by growth factors. Spine 16(3):253-260.
47. Tim Yoon S, Su Kim K, Li J, Soo Park J, Akamaru T, Elmer WA, Hutton WC. 2003. The effect of bone morphogenetic protein-2 on rat intervertebral disc cells in vitro. Spine 28(16):1773-1780.
48. Tozum TF, Demiralp B. 2003. Platelet-rich plasma: A promising innovation in dentistry. J Can Dent Assoc 69(10):664.
49. Urban JP, Maroudas A. 1981. Swelling of the intervertebral discin vitro. Connect Tissue Res 9(1):1-10.
50. Wadhwa M, Seghatchian MJ, Lubenko A, Contreras M, Dilger P, Bird C, Thorpe R. 1996. Cytokine levels in platelet concentrates: Quantitation by bioassays and immunoassays. Br J Haematol 93(1):225-234.
51. Wang JY, Baer AE, Kraus VB, Setton LA. 2001. Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. Spine 26(16):1747-1751; discussion 1752.
52. Weibrich G, Kleis WK, Hafner G, Hitzler WE. 2002. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 30(2):97-102.
53. Yung Lee J, Hall R, Pelinkovic D, Cassinelli E, Usas A, Gilbertson L, Huard J, Kang J. 2001. New use of a three-dimensional pellet culture system for human intervertebral disc cells: Initial characterization and potential use for tissue engineering. Spine 26(21):2316-2322.