Real-time PCR assay to quantify Fusarium graminearum wild-type and recombinant mutant DNA in plant material

Journal of Microbiological Methods

Volumn 67, Issue 3, 2006, Pages 534-542

  Dyer, Rex B ^a   Kendra, David F ^a   Brown, Daren W ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Fusarium graminearum; Fusarium head blight; Plant pathogen; Quantitative assay; Real time PCR; SYBR green; Wheat scab

Indexed keywords

BIOLOGICAL MARKER; GENOMIC DNA; MUTANT PROTEIN; RECOMBINANT DNA;

ARTICLE; FUNGAL STRAIN; FUNGUS MUTANT; FUSARIUM GRAMINEARUM; GENE DELETION; NONHUMAN; PLANT DISEASE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; WILD TYPE;

COLONY COUNT, MICROBIAL; DNA, FUNGAL; DNA, RECOMBINANT; FUSARIUM; MUTATION; ORGANIC CHEMICALS; PLANT DISEASES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEEDS; SENSITIVITY AND SPECIFICITY; TRITICUM;

FUSARIUM; GIBBERELLA ZEAE; TRITICUM AESTIVUM;

EID: 33750316809     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2006.05.011     Document Type: Article

References (37)

1. Bai G.-H., and Shaner G. Scab of wheat: prospects for control. Plant Dis. 78 (1994) 760-766
2. Bai G.-H., and Shaner G. Variation in Fusarium graminearum and cultivar resistance in wheat scab. Plant Dis. 80 (1996) 975-979
3. Bai G.-H., Desjardins A.E., and Plattner R.D. Deoxynivalenol-nonproducing Fusarium graminearum causes initial infection, but does not cause disease spread in wheat spikes. Mycopathologia 153 (2001) 91-98
4. Bowden R.L., and Leslie J.F. Nitrate non-utilizing mutants of Gibberella zeae and their use in determining vegetative compatibility. Exp. Mycol. 16 (1992) 308-315
5. Bustin S.A., and Nolan T. Chemistries. In: Bustin S.A. (Ed). A-Z of Quantitative PCR (2004), International University Line, La Jolla, CA 215-263
6. Bustin S.A., and Nolan T. Data analysis and interpretation. In: Bustin S.A. (Ed). A-Z of Quantitative PCR (2004), International University Line, La Jolla, CA 441-482
7. Brown D.W., Dyer R.B., McCormick S.P., Kendra D.F., and Plattner R.D. Functional demarcation of the Fusarium core trichothecene gene cluster. Fungal Genet. Biol. 41 (2004) 454-462
8. Desjardins A.E., Proctor R.H., Bai G.-H., McCormick S.P., Shaner G., Buechley G., and Hohn T.M. Reduced virulence of trichothecene-nonproducing mutants of Gibberella zeae in wheat field tests. Mol. Plant-Microb. Interact. 9 9 (1996) 775-781
9. Desjardins A.E., Bai G.-H., Plattner R.D., and Proctor R.H. Analysis of aberrant virulence of Gibberella zeae following transformation-mediated complementation of a trichothecene-deficient (tri5) mutant. Microbiology 146 8 (2000) 2059-2068
10. Desjardins A.E., Brown D.W., Yun S.-H., Proctor R.H., Lee T., Plattner R.D., Lu S.-W., and Turgeon G.B. Deletion and complementation of the mating type (MAT) locus of the wheat head blight pathogen Gibberella zeae. Appl. Environ. Microbiol. 70 (2004) 2437-2444
11. Dyer R.B., Plattner R.D., Kendra D.F., and Brown D.W. Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitro. J. Agric. Food Chem. 53 (2005) 9281-9287
12. Fincham J.R.S. Transformation in fungi. Microbiol. Rev. 53 (1989) 148-170
13. Guenther J.C., and Trail F. The development and differentiation of Gibberella zeae (anamorph: Fusarium graminearum) during colonization of wheat. Mycologia 97 1 (2005) 229-237
14. Hou Z., Xue C., Peng Y., Katan T., Kistler H.C., and Xu J.-R. A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation and plant infection. Mol. Plant-Microb. Interact. 15 11 (2002) 1119-1127
15. Jenczmionka N.J., and Schafer W. The GpmK1 kinase of Fusarium graminearum regulates the induction of specific secreted enzymes. Curr. Genet. 47 (2004) 29-36
16. Jenczmionka N.J., Maier F.J., Losch A.P., and Schafer W. Mating, conidiation and pathogenicity of Fusarium graminearum, the main causal agent of the head-blight disease of wheat, are regulated by the MAP kinase gpmk1. Curr. Genet. 43 (2003) 87-95
17. Kruger W.M., Pritsch C., Chao S., and Muehlbauer G.J. Functional and comparative bioinformatic analysis of expressed genes from wheat spikes infected with Fusarium graminearum. Mol. Plant-Microb. Interact. 15 5 (2002) 445-455
18. Lu S.-W., Kroken S., Lee B.-N., Robbertse B., Churchill A.C.L., and Yoder O.C. A novel class of gene controlling virulence in plant pathogenic ascomycete fungi. Proc. Natl. Acad. Sci. U. S. A. 100 10 (2003) 5980-5985
19. Miller J.D., and Ewen M.A. Toxic effects of deoxynivalenol on ribosomes and tissues of the spring wheat cultivars Frontana and Casavant. Nat. Toxins 5 6 (1997) 234-237
20. Miller S.S., Chabot D.M.P., Ouellet T., Harris L.J., and Fedak G. Use of a Fusarium graminearum strain transformed with green fluorescent protein to study infection in wheat (Triticum aestivum). Can. J. Plant Pathol. 26 (2004) 453-463
21. Nicholson P., Simpson D.R., Weston G., Rezanoor H., Lees N., Parry A.K., and Joyce D.W. Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiol. Mol. Plant Pathol. 53 (1998) 17-37
22. Pritsch C., Muehlbauer G.J., Bushnell W.R., Somers D.A., and Vance C.P. Fungal development and induction of defense response genes during early infection of wheat spikes by Fusarium graminearum. Mol. Plant-Microb. Interact. 13 2 (2000) 159-169
23. Pritsch C., Vance C.P., Bushnell W.R., Somers D.A., Hohn T.M., and Muehlbauer G.J. Systemic expression of defense response genes in wheat spikes as a response to Fusarium graminearum infection. Physiol. Mol. Plant Pathol. 58 (2001) 1-12
24. Proctor R.H., Hohn T.M., and McCormick S.P. Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol. Plant-Microb. Interact. 8 4 (1995) 593-601
25. Reischer G.H., Lemmens M., Farnleitner A., Adler A., and Mach R.L. Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe. J. Microbiol. Methods 59 (2004) 141-146
26. Ribichich K.F., Lopez S.E., and Vegetti A.C. Histopathological spikelet changes produced by Fusarium graminearum in susceptible and resistant wheat cultivars. Plant Dis. 84 7 (2000) 794-802
27. Rocha O., Ansari K., and Doohan F.M. Effects of trichothecene mycotoxins on eukaryotic cells: a review. Food Addit. Contam. 22 4 (2005) 369-378
28. Schnerr H., Niessen L., and Vogel R.F. Real time detection of the tri5 gene in Fusarium species by LightCycler-PCR using SYBR Green I for continuous fluorescence monitoring. Int. J. Food Microbiol. 71 (2001) 53-61
29. Schnerr H., Vogel R.F., and Niessen L. Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat samples. Lett. Appl. Microbiol. 35 (2002) 121-125
30. Seong K., Hou Z., Tracy M., Kistler H.C., and Xu J.-R. Random insertional mutagenesis identifies genes associated with virulence in the wheat scab fungus Fusarium graminearum. Phytopathology 95 (2005) 744-750
31. Skadsen R.W., and Hohn T.M. Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis. Physiol. Mol. Plant Pathol. 64 (2004) 45-53
32. Snijders C.H.A. Fusarium head blight and mycotoxin contamination of wheat, a review. Neth. J. Plant Pathol. 96 (1990) 187-198
33. Trail F., Xu J.-R., San Miguel P., Halgren R.G., and Kistler H.C. Analysis of expressed sequence tags from Gibberella zea (anamorph Fusarium graminearum). Fungal Genet. Biol. 28 (2003) 187-197
34. Tuite J., Shaner G., and Everson R.J. Wheat scab in soft red winter wheat in Indiana in 1986 and its relation to some quality measurements. Plant Dis. 74 (1990) 959-962
35. Urban M., Mott E., Farley T., and Hammond-Kosack K. The Fusarium graminearum MAP1 gene is essential for pathogenicity and development of perithecia. Mol. Plant Pathol. 4 5 (2003) 347-359
36. Voigt C.A., Schafer W., and Salomon S. A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals. Plant J. 42 (2005) 364-375
37. Waalwijk C., van der Heide R., De vries I., Van der Lee T., Schoen C., Costrel-de Corainville G., Hauser-Hahn I., Kastelein P., Kohl J., Lonnet P., Demarquet T., and Kema G.H.J. Quantitative detection of Fusarium species in wheat using TaqMan. Eur. J. Plant Pathol. 110 (2004) 481-494