Preventive effects of Azadirachta indica on benzo(a)pyrene-DNA adduct formation in murine forestomach and hepatic tissues

Phytotherapy Research

Volumn 20, Issue 10, 2006, Pages 889-895

  Gangar, Subhash Chander ^a   Sandhir, Rajat ^a   Rai, Durg Vijay ^a   Koul, Ashwani ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Azadirachta indica; Benzo(a)pyrene; Biotransformation enzymes; DNA adducts; Glutathione

Indexed keywords

AZADIRACHTA INDICA EXTRACT; BENZO[A]PYRENE; CYTOCHROME B5; CYTOCHROME P450; GLUTATHIONE; GLUTATHIONE TRANSFERASE; LACTATE DEHYDROGENASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AZADIRACHTA INDICA; BIOTRANSFORMATION; CONTROLLED STUDY; DNA ADDUCT; DRUG EFFECT; FEMALE; FORESTOMACH; LIVER; METABOLIC ACTIVATION; MOUSE; NONHUMAN; PLANT LEAF;

ANIMALS; ANTIMUTAGENIC AGENTS; AZADIRACHTA; BENZO(A)PYRENE; CYTOCHROME P-450 ENZYME SYSTEM; CYTOCHROMES B5; DNA ADDUCTS; GLUTATHIONE; GLUTATHIONE TRANSFERASE; L-LACTATE DEHYDROGENASE; LIVER; MICE; MICE, INBRED BALB C; PLANT EXTRACTS; PLANT LEAVES; STOMACH;

AZADIRACHTA INDICA; MURINAE;

EID: 33750216494     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.1967     Document Type: Article

References (38)

1. Abraham SK, Singh SP. 1999. Anti-genotoxicity and glutathione-S- transferase activity in mice pretreated with caffeinated and decaffeinated coffee. Food Chem Toxicol 37: 733-739.
2. Anderson MW, Boroujerdi M, Wilson AGE. 1981. Inhibition in vivo of the formation of adducts between metabolites of benzo(a)pyrene and DNA by butylated hydroxyanisole. Cancer Res 41: 4309-4315.
3. Arivazhagan S, Velmurugan B, Bhuvaneswari V, Nagini S. 2004. Effect of aqueous extracts of garlic (Allium sativum) and neem (Azadirachta indica) leaf on hepatic and blood oxidant-antioxidant status during experimental gastric carcinogenesis. J Med Food 7: 334-339.
4. Balasenthil S, Arivazhagan S, Ramachandran CR, Ramachandran V, Nagini S. 1999. Chemopreventive potential of neem (Azadirachta indica) on 7,12-dimethylbenz[a]anthracene (DMBA) induced hamster buccal pouch carcinogenesis. J Ethnopharmacol 67: 189-195.
5. Bergmeyer HU, Bernt E (eds). 1971. In Methods in Enzymatic Analysis, vol. II. Academic Press: New York, 5574-5579.
6. Biswas K, Chattopadhyay I, Banerjee R, Bandopadhyay U. 2002. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci 82: 1336-1345.
7. Collins JF, Brown JP, Alexeeff GV, Salmon AG. 1998. Potency equivalency factors for some polycyclic aromatic hydrocarbons and polycyclic aromatic hydrocarbon derivatives. Regul Toxicol Pharmacol 28: 45-54.
8. Conney AH. 1982. Induction of microsomal enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons: G. H. A. Clowes Memorial Lecture. Cancer Res 42: 4875-4917.
9. Dasgupta T, Banerjee S, Yadava PK, Rao AR. 2004. Chemopreventive potential of Azadirachta indica (Neem) leaf extract in murine carcinogenesis model systems. J Ethnopharmacol 92: 23-36.
10. Dipple A. 1991. Polycyclic hydrocarbons and carcinogenesis. In Polycyclic Aromatic Hydrocarbon Carcinogenesis: An Introduction, Harvey RG (ed.). ACS Symposium Series 283: Washington, DC, 1-17.
11. Gangar SC, Sandhir R, Rai DV, Koul A. 2006. Modulatory effects of Azadirachta indica on benzo(a)pyrene induced forestomach tumorigenesis in Balb/c mice. World J Gastroenterol 12: 2749-2755.
12. Gelboin HV. 1980. Benzo(a)pyrene metabolism, activation and carcinogenesis: role and regulation of mixed function oxidases and related enzymes. Physiol Rev 60: 1107-1166.
13. Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione-S-transferase: The first enzymatic step in mercapturic acid formation. J Biol Chem 249: 7130-7139.
14. Hu X, Singh SV. 1997. Glutathione S-transferases of female A/J mouse lung and their induction by anticarcinogenic organosulfides from garlic. Arch Biochem Biophys 340: 279-286.
15. Jagetia GC, Baliga MS, Venkatesh P. 2003. Effect of Sapthaparna (Alstonia scholaris Linn) in modulating the benzo(a)pyrene-induced forestomach carcinogenesis in mice. Toxicol Lett 144: 183-193.
16. Koul A, Ghara AR, Gangar SC. 2006a. Chemomodulatory effects of Azadirachta indica on the hepatic status of skin tumor bearing mice. Phytother Res 20: 169-177.
17. Koul A, Mukherjee N, Gangar SC. 2006b. Inhibitory effects of Azadirachta indica on DMBA-induced skin carcinogenesis in Balb/c mice. Mol Cell Biochem 283: 47-55.
18. Koul A, Singh M, Gangar SC. 2005. Modulatory effect of different doses of alpha-tocopherol on benzo(a)pyrene-DNA adduct formation in the pulmonary tissue of cigarette smoke inhaling mice. Indian J Exp Biol 43: 1139-1143.
19. Kusamran WR, Ratanavila A, Tepsuwan A. 1998. Effects of neem flowers, Thai and Chinese bitter gourd fruits and sweet basil leaves on hepatic mono-oxygenases and glutathione S-transferase activities, and in vitro metabolic activation of chemical carcinogens in rats. Food Chem Toxicol 36: 475-484.
20. Lampe WJ, King BI, Li S et al. 2000. Brassica vegetables increase and apiaceous vegetables decrease cytochrome P450 1A2 activity in humans: changes in caffeine metabolite ratios in response to controlled vegetable diets. Carcinogenesis 21: 1157-1162.
21. 2-terminal kinase 1 by benzo(a)pyrene. Cancer Res 58: 2102-2106.
22. Lowry OH, Rosebrough NJ, Farr AN, Randall RJ. 1951. Protein measurement with Folin phenol reagent. J Biol Chem 193: 256-265.
23. Lu SC. 1999. Regulation of hepatic glutathione synthesis: current concepts and controversies. FASEB J 13: 1169-1183.
24. Moron MS, Depierre JW, Mannervik B. 1979. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta 582: 67-78.
25. Nakamura Y, Ohigashi H, Masuda S, et al. 2000. Redox regulation of glutathione-S-transferase induction by benzyl isothiocyanate: correlation of enzyme induction with the formation of reactive oxygen intermediates. Cancer Res 60: 219-225.
26. Omura J, Sato R. 1964a. The carbon monoxide binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239: 2370-2378.
27. Omura J, Sato R. 1964b. The carbon monoxide binding pigment of liver microsomes. II. Solubilization, purification, and properties. J Biol Chem 239: 2379-2385.
28. Porter TD. 2002. The roles of cytochrome b5 in cytochrome P450 reactions. J Biochem Mol Toxicol 16: 311-316.
29. Ray SD, Parikh H, Hickey E, Bagchi M, Bagchi D. 2001. Differential effects of IH636 grape seed proanthocyanidin extract and a DNA repair modulator 4-aminobenzamide on liver microsomal cytochrome 4502E1-dependent aniline hydroxylation. Mol Cell Biochem 218: 27-33.
30. Reddy AC, Lokesh BR. 1996. Effect of curcumin and eugenol on iron-induced hepatic toxicity in rats. Toxicol 107: 39-45.
31. Schoket B, Papp G, Levay K, Mrackova G, Kadlubar FF. 2001. Impact of metabolic genotypes on levels of biomarkers of genotoxic exposure. Mutat Res 482: 57-69.
32. Shimada T, Fujii-Kuriyama Y. 2004. Metabolic activation of polycyclic aromatic hydrocarbons to carcinogens by cytochromes P450 1A1 and 1B1. Cancer Sci 95: 1-6.
33. Singh SV, Hu X, Srivastva SK et al. 1998. Mechanism of inhibition of B(a)P-induced forestomach cancer in mice by dietary curcumin. Carcinogenesis 19: 1357-1360.
34. Subapriya R, Nagini S. 2003. Ethanolic neem leaf extract protects against N-methyl-N-nitro-N-nitrosoguanidine induced gastric carcinogenesis. Asian Pac J Cancer Prev 4: 215-223.
35. Subapriya R, Nagini S. 2005. Medicinal properties of neem leaves: a review. Curr Med Chem Anti-Cancer Agents 5: 149-156.
36. Talalay P, Fahey JW, Holtzclaw WD, Prestera T, Zhang Y. 1995. Chemoprotection against cancer by phase 2 enzyme induction. Toxicol Lett 82-83: 173-179.
37. Wilkinson J, Clapper M. 1997. Detoxification enzymes and chemoprevention. Proc Soc Exp Biol Med 216: 192-200.
38. Wogan GN, Gorelick NJ. 1985. Chemical and biochemical dosimetry of exposure of genotoxic chemicals. Environ Health Perspect 62: 5-18.