Analysis of cel and pel genes from Pectobacterium chrysanthemi PY35 for relatedness to pathogenicity

Journal of Microbiology and Biotechnology

Volumn 14, Issue 5, 2004, Pages 1047-1051

  Park, Sang Ryeol ^b   Lim, Woo Jin ^a   Kim, Min Keun ^a   Hong, Su Young ^a   Shin, Eun Chule ^a   Kim, Eun Ju ^a   Lee, Jong Yeoul ^b   Woo, Jong Gyu ^b   Kim, Hoon ^c   Yun, Han Dae ^a  

^a Gyeongsang National University   (South Korea)

^b National Institute of Animal Science   (South Korea)

^c Sunchon National University   (South Korea)

Author keywords

cel gene; Double mutant; Pathogenicity; Pectobacterium chrysanthemi; pel gene

Indexed keywords

CELLULASE; GENE PRODUCT; PECTATE LYASE; PROTEIN CEL; PROTEIN PEL; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CELL WALL; CONTROLLED STUDY; ENZYME ACTIVITY; ERWINIA; GENE IDENTIFICATION; GENE ISOLATION; GENE MUTATION; GENETIC RECOMBINATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT CELL; POTATO;

BACTERIA (MICROORGANISMS); ERWINIA CHRYSANTHEMI; SOLANUM TUBEROSUM;

EID: 8644271764     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Beaulieu, C., M. Boccara, and F. V. Gijsegem. 1993. Pathogenic behaviour of pectinase-defective Erwinia chrysanthemi mutants on different plants: Possible involvement of pectic enzymes in host specificity. Mol. Plant-Microbe Interact. 6: 197-202.
2. Beguin, P. 1990. Molecular biology of cellulose degradation. Annu. Rev. Microbiol. 44: 219-248.
3. Hinton, J. C. D., J. M. Sidebotham, D. R. Gill, and G. P. R. Salmond. 1989. Isolation and characterization of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence. Mol. Microbiol. 3: 1785-1795.
4. Hong, I. P., H. K. Jang, S. Y. Lee, and S. G. Choi. 2003. Cloning and characterization of a bifunctional cellulase-chitosanase gene from Bacillus licheniformis NBL420. J. Microbiol. Biotechnol. 13: 35-42.
5. Johnvesly, B., S. Virupakshi, G. N. Patil, A. Ramalingam, and G. R. Naik. 2002. Cellulase-free thermostable alkaline xylanase from thermophilic and alkalophilic Bacillus sp. JB-99. J. Microbiol. Biotechnol. 12: 153-156.
6. Kim, M. K., S. R. Park, S. J. Cho, W. J. Lim, S. K. Ryu, C. R. An, S. Y. Hong, Y. W. Park, G. G. Kahng, J. H. Kim, H. Kim, and H. D. Yun. 2002. The effect of the disrupted yhjQ on cellular morphology and cell growth in Escherichia coli. Appl. Microbiol. Biotechnol. 60: 134-138.
7. Koutonjansky, A., A. Diolez, M. Boccara, Y. Bertheau, T. Andro, and A. Coleno. 1985. Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes. EMBO J. 4: 781-785.
8. Lee, J. H. 2002. Importance of nucleotide adjacent to the core region of Diphtheria tox promoter/operator. J. Microbiol. Biotechnol. 12: 622-627.
9. Park, S. R., S. J. Cho, and H. D. Yun. 2000. Cloning and sequencing of pel gene responsible for CMCase activity from Erwinia chrysanthemi PY35. Biosci. Biotechnol. Biochem. 64: 925-930.
10. Park, S. R., M. K. Kim, J. O. Kim, D. W. Bae, S. J. Cho, Y U. Cho, and H. D. Yun. 2000. Characterization of Erwinia chrysanthemi PY35 cel and pel gene existing in tandem and rapid identification of their gene products. Biochem. Biophys. Res. Commun. 268: 420-425.
11. Park, S. R., M. K. Kim, J.O. Kim, S. J. Cho, Y. U. Cho, and H. D. Yun. 2000. Cloning and sequencing of cel5Z gene from Erwinia chrysanthemi PY35. Mol. Cells 10: 269-274.
12. Park, Y. W., S. T. Lim, S. J. Cho, and H. D. Yun. 1997. Characterization of Erwinia carotovora subsp. carotovora LY34 endo-β-1,4-glucanase genes and rapid identification of their gene products. Biochem. Biophys. Res. Commun. 241: 636-641.
13. Pissavin, C., J. Robert-Baudouy, and N. Hugouvieux-Cotte-Pattat. 1996. Regulation of pelZ, a gene of the pelBC cluster encoding a new pectate lyase in Erwinia chrysanthemi 3937. J. Bacteriol. 178: 7187-7196.
14. Roeder, D. L. and A. Collmer. 1985. Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi J. Bacteriol. 164: 51-56.
15. Ruvkun, G. B. and F. M. Ausubel. 1981. A general method of site-directed mutagenesis in prokaryotes. Nature (London) 289: 85-89.