Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems

Journal of Translational Medicine

Volumn 4, Issue , 2006, Pages

  Galeffi, Patrizia ^a   Lombardi, Alessio ^a   Pietraforte, Immacolata ^a,d   Novelli, Flavia ^a   Di Donato, Monica ^a   Sperandei, Maria ^a   Tornambé, Andrea ^a   Fraioli, Rocco ^b   Martayan, Aline ^b   Natali, Pier Giorgio ^b   Benevolo, Maria ^b   Mottolese, Marcella ^b   Ylera, Francisco ^c   Cantale, Cristina ^a   Giacomini, Patrizio ^b  

^a ENEA   (Italy)

^b REGINA ELENA NATIONAL CANCER INSTITUTE   (Italy)

^c ROCHE DIAGNOSTICS GMBH   (Germany)

^d ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2 ANTIBODY; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; STREPTAVIDIN; TOSYLCHLORAMIDE SODIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BACTERIUM; BINDING AFFINITY; BREAST CARCINOMA; CELL FREE SYSTEM; CONTROLLED STUDY; DIAGNOSTIC VALUE; DRUG BINDING SITE; DRUG SYNTHESIS; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; ISOTOPE LABELING; MOLECULAR CLONING; MOUSE; NONHUMAN; PROCESS OPTIMIZATION; SERODIAGNOSIS; TOBACCO; YEAST;

EID: 33749597339     PISSN: 14795876     EISSN: 14795876     Source Type: Journal    
DOI: 10.1186/1479-5876-4-39     Document Type: Article

References (30)

1. Mendelsohn J, Baselga J: The EGIF receptor family as targets for cancer therapy. Oncogene 2000, 19:6550-6556.
2. Dove A: Uncorking the biomanufacturing bottleneck. Not Biotechnol 2002, 20:777-779.
3. Ma JK-C, Drake PMW, Christou P: The production of recombinant pharmaceutical proteins in plants. Nat Rev Genet 2003, 4:794-805.
4. Benvenuto E, Ordas R, Tavazza R, Ancora G, Biocca S, Cattaneo A, Galeffi P: 'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants. Plant Mol Biol 19991, 17:965-874.
5. Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, Galeffi P: Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 1993, 366:469-472.
6. Ryabova LA, Desplancq D, Spirin AS, Pluckthun A: Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones. Nat Biotechnol 1997, 15:79-84.
7. Jermutus L, Ryabova LA, Plückthun A: Recent advances in producing and selecting functional proteins by using cell free translation. Curr Opin Biotechnol 1998, 9:534-548.
8. Merk H, Stiege W, Tsumoto K, Kumagai I, Erdmann VA: Cell-free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression. J Biochem 1999, 125:328-333.
9. Hoffmann M, Nemetz C, Madin K, Buchberger B: Rapid translation system: a novel cell-free way from gene to protein. Biotechnol Annu Rev 2004, 10:1-30.
10. Verch T, Yusibov V, Koprowski H: Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector. J Immunol Methods 1998, 220:69-75.
11. Stoger E, Vaquero C, Torres E, Sack M, Nicholson L, Drossard J, Williams S, Keen D, Perrin Y, Christou P, Fischer R: Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol Biol 2000, 42:583-590.
12. McCormick A, Reini S, Cameron T, Vojdani F, Fronefield M, Levy R, Tuse D: Individualized human scFv vaccines produced in plants: humoral anti-idiotype responses in vaccinated mice confirm relevance to the tumor Ig. J Immunol Methods 2003, 278:95-104.
13. Galeffi P,, Lombardi A, Donato MD, Latini A, Sperandei M, Cantale C, Giacomini P: Expression of single-chain antibodies in transgenic plants. Vaccine 2005, 23:1823-1827.
14. Lombardi A. Sperandei M, Cantale C, Giacomini P, Galeffi P: Functional expression of a single-chain antibody specific for the HER2 human oncogene in a bacterial reducing environment. Protein Expr Purif 2005, 44:10-15.
15. De Lorenzo C, Palmer DB, Piccoli R, Ritter MA, D'Alessio G: A new human antitumor immunoreagent specific for Erb82. Clin Cancer Res 2002, 8:1710-1719.
16. Azemar M, Djahansouzi S, Jager E, Solbach C, Schmidt M, Maurer AB, Mross K, Unger C, von Minckwitz G, Dall P, Groner B, Weis WS: Regression of cutaneous tumor lesions in patients intratumorally injected with a recombinant single-chain antibody-toxin targeted to ErbB2/HER2. Breast Cancer Res Treat 2003, 82:155-164.
17. Cheng LS, Liu AP, Yang JH, Dong YQ Li LW, Wang J, Wang CC, Liu J: Construction, expression and characterization of the engineered antibody against tumor surface antigen, p185 (cerbB-2). Cell Res 2003, 13:35-48.
18. Kim DM, Swartz JR: Efficient production of a bioactive, multiple disulfide-bonded protein. Biotechnol Bioeng 2004, 85:122-129.
19. Digiesi G, Giacomini P, Fraioli R, Mariani M, Nicotra MR, Segatto O, Natali PG: Production and characterization of murine mAbs to the extracellular domain of human neu oncogene product GP185HER2. Hybridoma 1992, 11:519-527.
20. Giacomini P, Segatto O, Natali PG: Multiple epitope recognition: an approach to improved radioimmunodetection of tumor associated antigens. Int J Cancer 1987, 39:729-736.
21. Alimandi M, Wang L, Bottaro D, Lee C, Kuo A, Frankel M, Fedi P, Tang C, Lippman M, Pierce J: Epidermal growth factor and beta-cellulin mediate signal transduction through co-expressed ErbB2 and ErbB3 receptors. EMBO J 1997, 16:5608-5617.
22. Galeffi P, Giunta G, Guida S, Cantalle C: Engineering of a single chain variable fragment antibody specific for the Citrus tristeza. virus and its expression in Escherichia coli and Nicotiana tabacum. Eur J Plant Pathol 2002, 108:479-483.
23. Draper J, Scott R, Armitage P, Walden R: Plant genetic transformation and gene expression. A laboratory manual Oxford: Blackwell Scientific Publication. The Alden Press; 1988.
24. Chapman S, Kavanagh T, Baulcombe D: Potato virus X as a vector for gene expression in plants. Plant J 1992, 2:549-557.
25. [http://www.proteinexpression.com].
26. Harlow E, Lane D: Antibodies: a laboratory manual New York: Cold Spring Harbor Laboratory Press; 1988.
27. Mattheakis LC, Bhatt RR. Dower WJ: An in vitro polysome display system for identifying ligands from very large peptide libraries. Proc Natl Acad Sci 1994, 91:9022-9026.
28. Hanes J, Pluckthun A: In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci 1997, 94:4937-4942.
29. Martineau P, Jones P, Winter G: Expression of an antibody fragment at high levels in the bacterial cytoplasm. J Mol Biol 1998, 280:117-127.
30. Fruci D, Ferracuti S, Limongi MZ, Cunsolo V, Giorda E, Fraioli R, Sibilio L, Carroll O, Hattori A, van Endert PM, Giacomini P: Expression of endoplasmic reticulum anninopeptidases in EBV-B cell lines from healthy donors and in leukemia/lymphoma, carcinoma, and melanoma cell lines. J Immunol 2006, 176:4869-4879.