Expression of reconstructive hFVIII in the hrDNA by using hrDNA targeting vector

Chinese Science Bulletin

Volumn 50, Issue 19, 2005, Pages 2187-2192

  Liu, Xionghao ^a   Liu, Mujun ^a   She, Hua ^a   Wen, Lu ^a   Xue, Zhigang ^a   Liang, Desheng ^a   Cai, Fang ^a   Pan, Qian ^a   Long, Zhigao ^a   Wu, Lingqian ^a   Dai, Heping ^a   Xia, Kun ^a   Xia, Jiahui ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Human derived vector; Reconstructive hFVIII; Site specific integration; Targeting expression

Indexed keywords

EID: 33749492411     PISSN: 10016538     EISSN: 18619541     Source Type: Journal    
DOI: 10.1360/982005-987     Document Type: Article

References (22)

1. Chen, C., Fang, X. D., Zhu, J. et al., The gene expression of coagulation factor VIII in mammalian cell lines, Thrombosis Res., 1999, 95: 105-115.
2. Plantier, J. L., Rodriguez, M. H., Enjolras, N. et al., A factor VIII minigene comprising the truncated intron I of factor IX highly improves the in vitro production of factor VIII, Thromb. Haemost., 2001, 86(2): 596-603.
3. Lynch, C. M., Israel, D. I., Kaufman, R. J. et al. Sequences in the coding region of clotting factor VIII act as dominant inhibitors of RNA accumulation and protein production, Hum. Gene Ther., 1993, 4(3): 259-272.
4. Hoeben, R. C., Fallaux, F. J., Cramer, S. J. et al. Expression of the blood-clotting factor-VIII cDNA is repressed by a transcriptional silencer located in its coding region, Blood, 1995, 85(9): 2447-2454.
5. Kaufman, R. J., Advances toward gene therapy for hemophilia at the millennium, Hum. Gene Ther., 1999, 10(13): 2091-2107.
6. Kaufman, R. J., Pipe, S. W., Regulation of factor VIII expression and activity by von Willebrand factor, Thromb. Haemost., 1999, 82(2): 201-208.
7. Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989.
8. Brown, J. P., Wei, W., Sedivy, J. M., Bypass of senescence after disruption of p21 CIP1/WAF1 gene in normal diploid human fibroblasts, Science, 1997, 277(5327): 831-834.
9. Arbones, M. L., Austin, H. A., Capon, D. J. et al., Gene targeting in normal somatic cells: Inactivation of the interferon-gamma receptor in myoblasts, Nat. Genet., 1994, 6(1): 90-97.
10. Hanson, K. D., Sedivy, J. M., Analysis of biological selections for high efficiency gene targeting, Mol. Cell Biol., 1995, 15(1): 45-51.
11. opes, T. S., Klootwijk, J., Veenstra, A. E. et al., High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: A new vector for high-level expression, Gene, 1989, 79(2): 199-206.
12. Wery, J., Gutker, D., Renniers, A. C. et al., High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma, Gene, 1997, 184(1): 89-97.
13. Santos, W. G., Buck, G. A., Simultaneous stable expression of neomycin phosphotransferase and green fluorescence protein genes in Trypanosoma cruzi, J. Parasitol., 2000, 86(6): 1281-1288.
14. Steele, R. E., Bakken, A. H., Reeder, R. H., Plasmids containing mouse rDNA do not recombine with cellular ribosomal genes when introduced into cultured mouse cells, Mol. Cell Bjol., 1984, 4(4): 576-582.
15. Stewart, S., MacDonald, N., Perkins, E. et al., Retrofitting of a satellite repeat DNA-based murine artificial chromosome (ACes) to contain loxP recombination sites, Gene Ther., 2002, 9(11): 719-723.
16. Sedivy, J. M., Dutriaux, A., Gene targeting and somatic cell genetics-A rebirth or a coming of age? Trends Genet., 1999, 15(3): 88-90.
17. Szostak, J. W., Wu, R., Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature, 1980, 284(5755): 426-430.
18. Brooks, A. R., Harkins, R. N., Wang, P., Transcriptional silencing is associated with extensive methylation of the CMV promoter following adenoviral gene delivery to muscle, J. Gene Med., 2004, 6(4): 395-404.
19. Richards, B., Zharkikh, L., Hsu, F. et al., Stable expression of Anthozoa fluorescent proteins in mammalian cells, Cytometry, 2002, 48(2): 106-112.
20. Fleury, S., Simeoni, E., Zuppinger, C., Multiply attenuated, self-inactivating lentiviral vectors efficiently deliver and express genes for extended periods of time in adult rat cardiomyocytes in vivo. Circulation, 2003, 107(18): 2375-2382.
21. Chen, L., Chen, H. M., Zou, B. Y. et al., Preparation of rAAV/hFIX by HSV/AAV hybrid helper virus and evaluation of its safety, Chinese Science Bulletin, 2003, 48(13): 1369-1374.
22. Jenke, A. C., Scinteie, M. F., Stehle, I. M., Expression of a transgene encoded on a non-viral episomal vector is not subject to epigenetic silencing by cytosine methylation, Mol. Biol. Rep., 2004, 31(2): 85-90.