A two-step method for the introduction of single or multiple defined point mutations into the genome of Saccharomyces cerevisiae

Yeast

Volumn 23, Issue 11, 2006, Pages 825-831

  Toulmay, Alexander ^a   Schneiter, Roger ^a  

^a UNIVERSITY OF FRIBOURG   (Switzerland)

Author keywords

Homologous recombination; PCR; Plasma membrane ATPase; Site directed mutagenesis; Yeast

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ALANINE; AMINO ACID; FUNGAL PROTEIN; GLYCINE; PROLINE; SERINE;

ALLELE; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL MEMBRANE; CELL POPULATION; CONTROLLED STUDY; FUNGAL GENE; FUNGAL GENETICS; FUNGAL STRAIN; FUNGUS GROWTH; GENE AMPLIFICATION; GENE EXPRESSION; GENE LOCUS; GENE MUTATION; GENETIC ANALYSIS; HAPLOIDY; HOMOLOGOUS RECOMBINATION; MARKER GENE; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PLASMID; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN MOTIF; PROTEIN STABILITY; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION; TEMPERATURE SENSITIVITY; UNTRANSLATED REGION; WILD TYPE;

ALLELES; GENOME, FUNGAL; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTON-TRANSLOCATING ATPASES; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 33748933575     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1397     Document Type: Article

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