Molecular determinants of dihydrouridine synthase activity

FEBS Letters

Volumn 580, Issue 22, 2006, Pages 5198-5202

  Savage, Dan F ^a   de Crécy Lagard, Valérie ^b   Bishop, Anthony C ^a  

^a AMHERST COLLEGE   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

Dihydrouridine; Dihydrouridine synthase; Site directed mutagenesis; tRNA; tRNA modification; YjbN

Indexed keywords

AMINO ACID; BACTERIAL ENZYME; DIHYDROURIDINE SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; AQUIFEX AEOLICUS; ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE IDENTIFICATION; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC COMPLEMENTATION TEST; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PROTEIN STRUCTURE, TERTIARY; URIDINE;

AQUIFEX AEOLICUS; ESCHERICHIA COLI;

EID: 33748798227     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.08.062     Document Type: Article

References (21)

1. Sprinzl M., Horn C., Brown M., Ioudovitch A., and Steinberg S. Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res. 26 (1998) 148-153
2. McCloskey J.A., and Crain P.F. The RNA modification database-1998. Nucleic Acids Res. 26 (1998) 196-197
3. Grosjean H., Sprinzl M., and Steinberg S. Posttranscriptionally modified nucleosides in transfer RNA: their locations and frequencies. Biochimie 77 (1995) 139-141
4. Bjork G.R., Ericson J.U., Gustafsson C.E., Hagervall T.G., Jonsson Y.H., and Wikstrom P.M. Transfer RNA modification. Annu. Rev. Biochem. 56 (1987) 263-287
5. Dalluge J.J., Hashizume T., Sopchik A.E., McCloskey J.A., and Davis D.R. Conformational flexibility in RNA: the role of dihydrouridine. Nucleic Acids Res. 24 (1996) 1073-1079
6. Dalluge J.J., Hamamoto T., Horikoshi K., Morita R.Y., Stetter K.O., and McCloskey J.A. Posttranscriptional modification of tRNA in psychrophilic bacteria. J. Bacteriol. 179 (1997) 1918-1923
7. Xing F., Martzen M.R., and Phizicky E.M. A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA. RNA. 8 (2002) 370-381
8. Bishop A.C., Xu J., Johnson R.C., Schimmel P., and de Crécy-Lagard V. Identification of the tRNA-dihydrouridine synthase family. J. Biol. Chem. 277 (2002) 25090-25095
9. Guzman L.M., Belin D., Carson M.J., and Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter. J. Bacteriol. 177 (1995) 4121-4130
10. Jacobson M., and Hedgcoth C. Determination of 5,6-dihydrouridine in ribonucleic acid. Anal. Biochem. 34 (1970) 459-469
11. Hunninghake D., and Grisolia S. A sensitive and convenient micromethod for estimation of urea, citrulline, and carbamyl derivatives. Anal. Biochem. 16 (1966) 200-205
12. Morgan-Kiss R.M., Wadler C., and Cronan J.E. Long-term and homogeneous regulation of the Escherichia coli araBAD promoter by use of a lactose transporter of relaxed specificity. Proc. Natl. Acad. Sci. USA 99 (2002) 7373-7377
13. Kato T., et al. A novel human tRNA-dihydrouridine synthase involved in pulmonary carcinogenesis. Cancer Res. 65 (2005) 5638-5646
14. Park F., et al. The 1.59 A resolution crystal structure of TM0096, a flavin mononucleotide binding protein from Thermotoga maritima. Proteins 55 (2004) 772-774
15. Jensen K.F., and Bjornberg O. Evolutionary and functional families of dihydroorotate dehydrogenases. Paths to Pyrimidines 6 (1998) 20-28
16. Rowland P., Norager S., Jensen K.F., and Larsen S. Structure of dihydroorotate dehydrogenase B: electron transfer between two flavin groups bridged by an iron-sulphur cluster. Structure Fold. Des. 8 (2000) 1227-1238
17. Marcinkeviciene J., Tinney L.M., Wang K.H., Rogers M.J., and Copeland R.A. Dihydroorotate dehydrogenase B of Enterococcus faecalis. Characterization and insights into chemical mechanism. Biochemistry 38 (1999) 13129-13137
18. Rosenbaum K., Jahnke K., Curti B., Hagen W.R., Schnackerz K.D., and Vanoni M.A. Porcine recombinant dihydropyrimidine dehydrogenase: comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes. Biochemistry 37 (1998) 17598-175609
19. Bjornberg O., Jordan D.B., Palfey B.A., and Jensen K.F. Dihydrooxonate is a substrate of dihydroorotate dehydrogenase (DHOD) providing evidence for involvement of cysteine and serine residues in base catalysis. Arch. Biochem. Biophys. 391 (2001) 286-294
20. Xing F., Hiley S.L., Hughes T.R., and Phizicky E.M. The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs. J. Biol. Chem. 279 (2004) 17850-17860
21. Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.E., Pettersson B., and Uhlen M. The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP). Genome Res. 14 (2004) 221-227