Cloning and sequencing of the Bet v 1-homologous allergen Fra a 1 in strawberry (Fragaria ananassa) shows the presence of an intron and little variability in amino acid sequence

Molecular Immunology

Volumn 44, Issue 6, 2007, Pages 1245-1252

  Musidlowska Persson, Anna ^a   Alm, Rikard ^a   Emanuelsson, Cecilia ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Allergen; Allergy; Birch pollen; Cross reactivity; Isoform; Mass spectrometry

Indexed keywords

BET V 1 ALLERGEN; FOOD ALLERGEN; FRA A 1 ALLERGEN; IMMUNOGLOBULIN E; ISOPROTEIN; POLLEN ANTIGEN; UNCLASSIFIED DRUG;

ALLERGY; AMINO ACID SEQUENCE; ANTIGEN ANTIBODY REACTION; ARTICLE; DNA SEQUENCE; ESCHERICHIA COLI; FOOD ALLERGY; GENE SEQUENCE; GENOMICS; INTRON; MASS SPECTROMETRY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN VARIANT; STRAWBERRY;

ALLERGENS; AMINO ACID SEQUENCE; ANTIGENS, PLANT; BASE SEQUENCE; CLONING, MOLECULAR; FRAGARIA; GENETIC VARIATION; INTRONS; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; VARIATION (GENETICS);

ESCHERICHIA COLI; FRAGARIA X ANANASSA; MALUS X DOMESTICA;

EID: 33748777681     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molimm.2006.06.004     Document Type: Article

References (27)

1. Aalberse R.C., Akkerdaas J., and van Ree R. Cross-reactivity of IgE antibodies to allergens. Allergy 56 (2001) 478-490
2. Bjerrum O.J., and Schafer Nielsen C. Buffer systems and transfer parameters for semidry electroblotting with a horizontal apparatus. In: Dunn M.J. (Ed). Electrophoresis-86; Fifth Meeting of the International Electrophoresis Society (1986), VCH, Weinhem, Germany, London 315-327
3. Blom N., Gammeltoft S., and Brunak S. Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J. Mol. Biol. 294 (1999) 1351-1362
4. Breiteneder H., and Ebner C. Molecular and biochemical classification of plant-derived food allergens. J. Allergy Clin. Immunol. 106 (2000) 27-36
5. Ferreira F., Hirtenlehner K., Jilek A., Godnik-Cvar J., Breiteneder H., Grimm R., Hoffmann-Sommergruber K., Scheiner O., Kraft D., Breitenbach M., Rheinberger H.J., and Ebner C. Dissection of immunoglobulin E and T lymphocyte reactivity of isoforms of the major birch pollen allergen Bet v 1: potential use of hypoallergenic isoforms for immunotherapy. J. Exp. Med. 183 (1996) 599-609
6. Gao Z.S., van de Weg W.E., Schaart J.G., Schouten H.J., Tran D.H., Kodde L.P., van der Meer I.M., van der Geest A.H., Kodde J., Breiteneder H., Hoffmann-Sommergruber K., Bosch D., and Gilissen L.J. Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica). Theor. Appl. Genet. 111 (2005) 171-183
7. Gobom J., Nordhoff E., Mirgorodskaya E., Ekman R., and Roepstorff P. Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry. J. Mass Spectrom. 34 (1999) 105-116
8. Goodall G.J., and Filipowicz W. Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants. EMBO J. 10 (1991) 2635-2644
9. Harndahl U., Tufvesson E., and Sundby C. The chloroplast small heat shock protein-purification and characterization of pea recombinant protein. Protein Expr. Purif. 14 (1998) 87-96
10. Hjernø K., Alm R., Canbäck B., Matthiesen R., Trajkovski K., Björk L., Roepstorff P., and Emanuelsson C.S. Down-regulation of the strawberry Bet v 1-homologous allergen in concert with the flavonoid biosynthesis pathway in colourless strawberry mutant. Proteomics 6 (2006) 1574-1587
11. Hoffmann-Sommergruber K., Vanek-Krebitz M., Radauer C., Wen J., Ferreira F., Scheiner O., and Breiteneder H. Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogenesis-related proteins share intron positions. Gene 197 (1997) 91-100
12. Horton R.M., Ho S.N., Pullen J.K., Hunt H.D., Cai Z., and Pease L.R. Gene splicing by overlap extension. Meth. Enzymol. 217 (1993) 270-279
13. Hsieh L.S., Moos Jr. M., and Lin Y. Characterization of apple 18 and 31 kd allergens by microsequencing and evaluation of their content during storage and ripening. J. Allergy Clin. Immunol. 96 (1995) 960-970
14. Jensen O.N. Modification-specific proteomics: characterization of post-translational modifications by mass spectrometry. Curr. Opin. Chem. Biol. 8 (2004) 33-41
15. Karamloo F., Scheurer S., Wangorsch A., May S., Haustein D., and Vieths S. Pyr c 1, the major allergen from pear (Pyrus communis), is a new member of the Bet v 1 allergen family. J. Chromatogr. B. Biomed. Sci. Appl. 756 (2001) 281-293
16. Karlsson A.L., Alm R., Ekstrand B., Fjelkner-Modig S., Schiott A., Bengtsson U., Bjork L., Hjerno K., Roepstorff P., and Emanuelsson C.S. Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens. Allergy 59 (2004) 1277-1284
17. Larsen M.R., and Roepstorff P. Mass spectrometric identification of proteins and characterization of their post-translational modifications in proteome analysis. Fresenius J. Anal. Chem. 366 (2000) 677-690
18. Mann M., and Jensen O.N. Proteomic analysis of post-translational modifications. Nat. Biotechnol. 21 (2003) 255-261
19. Marzban G., Puehringer H., Dey R., Brynda S., Ma Y., Martinelli A., Zaccarini M., van der Weg E., Housley Z., Kolarich D., Altmann F., and Laimer M. Localization and distribution of the major allergens in apple fruit. Plant Sci. 169 (2005) 387-394
20. Mills E., Madsen C., Shewry P., and Wichers H. Food allergens of plant origin-their molecular and evolutionary relationships. Trends Food Sci. Technol. 14 (2003) 145-156
21. Rappsilber J., Ishihama Y., and Mann M. Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal. Chem. 75 (2003) 663-670
22. Reuter A., Fortunato D., Garoffo L.P., Napolitano L., Scheurer S., Giuffrida M.G., Vieths S., and Conti A. Novel isoforms of Pru av 1 with diverging immunoglobulin E binding properties identified by a synergistic combination of molecular biology and proteomics. Proteomics 5 (2005) 282-289
23. Son D.Y., Scheurer S., Hoffmann A., Haustein D., and Vieths S. Pollen-related food allergy: cloning and immunological analysis of isoforms and mutants of Mal d 1, the major apple allergen, and Bet v 1, the major birch pollen allergen. Eur. J. Nutr. 38 (1999) 201-215
24. Swoboda I., Jilek A., Ferreira F., Engel E., Hoffmann-Sommergruber K., Scheiner O., Kraft D., Breiteneder H., Pittenauer E., Schmid E., et al. Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning. J. Biol. Chem. 270 (1995) 2607-2613
25. Swoboda I., Scheiner O., Heberlebors E., and Vicente O. cDNA cloning and characterization of 3 genes in the Bet-V-1 gene family that encode pathogenesis-related proteins. Plant Cell Environ. 18 (1995) 865-874
26. Vieths S., Scheurer S., and Ballmer-Weber B. Current understanding of cross-reactivity of food allergens and pollen. Ann. NY Acad. Sci. 964 (2002) 47-68
27. Vieths S., Schoning B., and Petersen A. Characterization of the 18 kDa apple allergen by two-dimensional immunoblotting and microsequencing. Int. Arch. Allergy Immunol. 104 (1994) 399-404