mRNA and protein expression and activities of nitric oxide synthases in the lumbar spinal cord of neonatal rats after sciatic nerve transection and melatonin administration

Neuroscience Letters

Volumn 407, Issue 2, 2006, Pages 182-187

  Rogério, Fábio ^a   Teixeira, Simone Aparecida ^b   Júnior, Hamilton Jordão ^a   Maria, Carla Cristina Judice ^a   Vieira, André Schwambach ^a   de Rezende, Alexandre César Santos ^a   Pereira, Gonçalo Amarante Guimarães ^a   Muscará, Marcelo Nicolás ^b   Langone, Francesco ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Immunohistochemistry; Neonatal rats; NOS activity; NOS isoforms; RT PCR; Sciatic nerve transection

Indexed keywords

ARGININE; CALCIUM; CITRULLINE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; INDUCIBLE NITRIC OXIDE SYNTHASE; MELATONIN; MESSENGER RNA; NEURONAL NITRIC OXIDE SYNTHASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DRUG EFFECT; ENDOTHELIUM CELL; ENZYME ACTIVITY; GENE EXPRESSION; IMMUNOHISTOCHEMISTRY; LUMBAR SPINAL CORD; MOTONEURON; NERVE FIBER TRANSECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCIATIC NERVE;

ANIMALS; ANIMALS, NEWBORN; AXOTOMY; IMMUNOHISTOCHEMISTRY; MELATONIN; NERVE TISSUE PROTEINS; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE I; RATS; RATS, WISTAR; RNA, MESSENGER; SCIATIC NERVE; SPINAL CORD;

EID: 33748688807     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2006.08.035     Document Type: Article

References (33)

1. Alderton W.K., Cooper C.E., and Knowles R.G. Nitric oxide synthases: structure, function and inhibition. Biochem. J. 357 (2001) 593-615
2. Bettahi I., Pozo D., Osuna C., Reiter R.J., Acuna-Castroviejo D., and Guerrero J.M. Melatonin reduces nitric oxide synthase activity in rat hypothalamus. J. Pineal Res. 20 (1996) 205-210
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
4. Bredt D.S. Endogenous nitric oxide synthesis: biological functions and pathophysiology. Free Radic. Res. 31 (1999) 577-596
5. Callsen-Cencic P., Hoheisel U., Kaske A., Mense S., and Tenschert S. The controversy about spinal neuronal nitric oxide synthase: under which conditions is it up- or down-regulated?. Cell Tissue Res. 295 (1999) 183-194
6. Capsoni S., Stankov B.M., and Fraschini F. Reduction of regional cerebral blood flow by melatonin in young rats. Neuroreport 6 (1995) 1346-1348
7. Castagné V., Gautschi M., Lefevre K., Posada A., and Clarke P.G.H. Relationships between neuronal death and the cellular redox status. Focus on the developing nervous system. Prog. Neurobiol. 59 (1999) 397-423
8. Chang H.M., Ling E.A., Lue J.H., Wen C.Y., and Shieh J.Y. Melatonin attenuates neuronal NADPH-d/NOS expression in the hypoglossal nucleus of adult rats following peripheral nerve injury. Brain Res. 873 (2000) 243-251
9. Estévez A.G., Kamaid A., Thompson J.A., Cornwell T.L., Radi R., Barbeito L., and Beckman J.S. Cyclic guanosine 5′ monophosphate (GMP) prevents expression of neuronal nitric oxide synthase and apoptosis in motor neurons deprived of trophic factors in rats. Neurosci. Lett. 326 (2002) 201-205
10. Hiki K., Hattori R., Kawai C., and Yui Y. Purification of insoluble nitric oxide synthase from rat cerebellum. J. Biochem. 111 (1992) 556-558
11. Huang P.L., and Lo E.H. Genetic analysis of NOS isoforms using nNOS and eNOS knockout animals. Prog. Brain Res. 118 (1998) 13-25
12. Jiang K.W., Gao F., Shui Q.X., Yu Z.S., and Xia Z.Z. Effect of diazoxide on regulation of vesicular and plasma membrane GABA transporter genes and proteins in hippocampus of rats subjected to picrotoxin-induced kindling. Neurosci. Res. 50 (2004) 319-329
13. Kano T., Shimizu-Sasamata M., Huang P.L., Moskowitz M.A., and Lo E.H. Effects of nitric oxide synthase gene knockout on neurotransmitter release in vivo. Neuroscience 86 (1998) 695-699
14. Kawai N., Bloch D.B., Filippov G., Rabkina D., Suen H.C., Losty P.D., Janssens S.P., Zapol W.M., Monte S., and Bloch K.D. Constitutive endothelial nitric oxide synthase gene expression is regulated during lung development. Am. J. Physiol. 268 (1995) L589-L595
15. Keilhoff G., Fansa H., and Wolf G. Neuronal NOS deficiency promotes apoptotic cell death of spinal cord neurons after peripheral nerve transection. Nitric Oxide 10 (2004) 101-111
16. Kroncke K.D., Suschek C.V., and Kolb-Bachofen V. Implications of inducible nitric oxide synthase expression and enzyme activity. Antioxid. Redox Signal. 2 (2000) 585-605
17. Lowrie M.B., and Lawson S.J. Cell death of spinal interneurons. Prog. Neurobiol. 61 (2000) 543-555
18. Lowrie M.B., and Vrbová G. Dependence of postnatal motoneurones on their targets: review and hypothesis. Trends Neurosci. 15 (1992) 80-84
19. Lundberg J.O.N., Farkas-Szallasi T., Weitzberg E., Rinder J., Lidholm J., Änggåard A., Hökfelt T., Lundberg J.M., and Alving K. High nitric oxide production in human paranasal sinuses. Nat. Med. 1 (1995) 370-373
20. Lyons C.R., Orloff G.J., and Cunningham J.M. Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J. Biol. Chem. 267 (1992) 6370-6374
21. Phifer C.B., and Terry L.M. Use of hypothermia for general anesthesia in preweanling rodents. Physiol. Behav. 38 (1986) 887-890
22. Piehl F., Hammarberg H., Tabar G., Hokfelt T., and Cullheimm S. Changes in the mRNA expression pattern, with special reference to calcitonin gene-related peptide, after axonal injuries in rat motoneurons depends on age and type of injury. Exp. Brain Res. 119 (1998) 191-204
23. Pozo D., Reiter R.J., Calvo J.P., and Guerrero J.M. Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum. Life Sci. 55 (1994) PL455-PL460
24. Reiter R.J. Oxidative damage in the central nervous system: protection by melatonin. Prog. Neurobiol. 56 (1998) 359-384
25. Rogério F., Queiroz L.S., Teixeira S.A., Oliveira A.L.R., Nucci G., and Langone F. Neuroprotective action of melatonin on neonatal rat motoneurons after sciatic nerve transection. Brain Res. 926 (2002) 33-41
26. Rogério F., Teixeira S.A., Rezende A.C.S., Sá R.C., Queiroz L.S., Nucci G., Muscará M.N., and Langone F. Superoxide dismutase isoforms 1 and 2 in lumbar spinal cord of neonatal rats after sciatic nerve transection and melatonin treatment. Brain Res. Dev. Brain Res. 154 (2005) 217-225
27. Swain M.G., Le T., Tigley A.W., and Beck P. Hypothalamic nitric oxide synthase is depressed in cholestatic rats. Am. J. Physiol. 272 (1997) G1034-G1040
28. Weinberg U. Evidence that melatonin retention by the neonatal rat is greatly increased as compared to the adult: a novel biochemical mechanism. Brain Res. 217 (1981) 221-224
29. Wong H., Anderson W.D., Cheng T., and Riabowol K.T. Monitoring mRNA expression by polymerase chain reaction: the "primer-dropping" method. Anal. Biochem. 223 (1994) 251-258
30. Wu W.T. Expression of nitric-oxide synthase (NOS) in injured CNS neurons as shown by NADPH diaphorase histochemistry. Exp. Neurol. 120 (1993) 153-159
31. Wu W., Liuzzi F.J., Schinco F.P., Depto A.S., Li Y., Mong J.A., Dawson T.M., and Snyder S.H. Neuronal nitric oxide synthase is induced in spinal neurons by traumatic injury. Neuroscience 61 (1994) 719-726
32. Xu M., Ng Y.K., and Leong S.K. Neuroprotective and neurodestructive functions of nitric oxide after spinal cord hemisection. Exp. Neurol. 161 (2000) 472-480
33. Yu W.H. Spatial and temporal correlation of nitric oxide synthase expression with CuZn-superoxide dismutase reduction in motor neurons following axotomy. Ann. N.Y. Acad. Sci. 962 (2002) 111-121