Inhibition of colon cancer (HT-29) cell proliferation by a triterpenoid isolated from Azadirachta indica is accompanied by cell cycle arrest and up-regulation of p21

Planta Medica

Volumn 72, Issue 10, 2006, Pages 917-923

  Roy, Molay Kumar ^a   Kobori, Masuko ^a   Takenaka, Makiko ^a   Nakahara, Kazuhiko ^b   Shinmoto, Hiroshi ^a   Tsushida, Tojiro ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b JAPAN INTERNATIONAL RESEARCH CENTER FOR AGRICULTURAL SCIENCES   (Japan)

Author keywords

Colon cancel; G0 G1 arrest; G2 M arrest; Nimbolide; p21

Indexed keywords

CHECKPOINT KINASE 2; CYCLIN D2; CYCLIN DEPENDENT KINASE 2; CYCLIN E; NIMBOLIDE; NUCLEIC ACID BINDING PROTEIN; PHOSPHATIDYLSERINE; PROTEIN P21; PROTEIN RAD 17; TRITERPENOID; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; AZADIRACHTA INDICA; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL DIFFERENTIATION; CELL MEMBRANE; CELL POPULATION; CELL PROLIFERATION; COLON CANCER; CONTROLLED STUDY; DOWN REGULATION; FLOW CYTOMETRY; GROWTH INHIBITION; HUMAN; HUMAN CELL; QUANTITATIVE ANALYSIS; UPREGULATION;

ALKALINE PHOSPHATASE; ANTINEOPLASTIC AGENTS; APOPTOSIS; AZADIRACHTA; CELL CYCLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYTOTOXINS; DRUG EVALUATION, PRECLINICAL; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, NEOPLASTIC; HT29 CELLS; HUMANS; LIMONINS; UP-REGULATION;

AZADIRACHTA INDICA;

EID: 33748627425     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2006-946694     Document Type: Article

References (27)

1. Keating B. Neem: The miraculous healing Herb. Winter Park: The neem association, 1994
2. Biswas K, Chattopadhyay I, Banerjee RK, Bandhopadhyay U. Biological activities and medicinal properties of Neem (Azadirachta indica). Curr Sci 2002; 82: 1336-45
3. Cohen E, Quistad GB, Casida JE. Cytotoxicity of nimbolide, epoxyazadiradione and other limonoids from neem insecticide. Life Sci 1996; 58: 1075-81
4. Glinsukon T, Somjaree R, Piyachaturawat P, Thebtaranonth Y. Acute toxicity of nimbolide and nimbic acid in mice, rats and hamsters. Toxicol Lett 1986; 30: 159-66
5. Martinez ME. Primary prevention of colorectal cancer: lifestyle, nutrition, exercise. Recent Results Cancer Res 2005; 166: 177-211
6. Cohen E, Quistad GB, Jeffries PR, Casida JE. Nimbolide is the principal cytotoxic component of neem seed insecticide preparations. Pestic Sci 1996; 48: 135-40
7. Kigodi PG, Blasko G, Thebtaranonth Y, Pezzuto JM, Cordell GA. Spectroscopic and biological investigation of nimbolide and 28-deoxonimbolide from Azadirachta indica. J Nat Prod 1989; 52: 1246-51
8. Reese DH, Fiorentino GJ, Claflin AJ, Malinin TI, Politano VA. Rapid induction of alkaline phosphatase activity by retinoic acid. Biochem Biophys Res Commun 1981; 102: 315-21
9. Cayrol C, Knibiehler M, Ducommun B. p21 binding to PCNA causes G1 and G2 cell cycle arrest in p53-deficient cells. Oncogene 1998; 16: 311-20
10. Medema RH, Klompmaker R, Smits VA, Rijksen G. p21waf1 can block cells at two points in the cell cycle, but does not interfere with processive DNA-replication or stress-activated kinases. Oncogene 1998; 16: 431-41
11. LaBaer J, Garrett MD, Stevenson LF, Slingerland JM, Sandhu C, Chou HS et al. New functional activities for the p21 family of CDK inhibitors. Genes Dev 1997; 11: 847-62
12. Cheng M, Olivier P, Diehl JA, Fero M, Roussel MF, Roberts JM et al. The p21(Cip1) and p27(Kip1) CDK 'inhibitors' are essential activators of cyclin D-dependent kinases in murine fibroblasts. EMBO J 1999; 18: 1571-83
13. Sherr CJ. Cancer cell cycles. Science 1996; 274: 1672-7
14. Morgan DO. Principles of CDK regulation. Nature 1995; 374: 131-4
15. Ding QM, Ko TC, Evers BM. Caco-2 intestinal cell differentiation is associated with G1 arrest and suppression of CDK2 and CDK4. Am J Physiol 1998; 275: C1193-200
16. Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R, Beach D. p21 is a universal inhibitor of cyclin kinases. Nature 1993; 366: 701-4
17. Zeng YX, el-Deiry WS. Regulation of p21WAF1/CIP1 expression by p53-independent pathways. Oncogene 1996; 12: 1557-64
18. Cappelletti V, Fioravanti L, Miodini P, Di Fronzo G. Genistein blocks breast cancer cells in the G(2)M phase of the cell cycle. J Cell Biochem 2000; 79: 594-600
19. Kehn K, Deng L, De La Fuente C, Strouss K, Wu K, Maddukuri A et al. The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cells. Retrovirology 2004; 1: 1-6
20. Sanchez Y, Wong C, Thoma RS, Richman R, Wu Z, Piwnica-Worms H et al. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 1997; 277: 1497-501
21. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282: 1893-7
22. Hirao A, Kong YY, Matsuoka S, Wakeham A, Ruland J, Yoshida H et al. DNA damage-induced activation of p53 by the checkpoint kinase Chk2. Science 2000; 287: 1824-7
23. Parsels LA, Parsels JD, Tai DC-H, Coughlin DJ, Maybaum J. 5-Fluoro-2″-deoxyuridine-induced cdc25A accumulation correlates with premature mitotic entry and clonogenic death in human colon cancer cells. Cancer Res 2004; 64: 6588-94
24. Chang KL, Kung ML, Chow NH, Su SJ. Genistein arrests hepatoma cells at G2/M phase: involvement of ATM activation and upregulation of p21waf1/cip1 and Weel. Biochem Pharmacol 2004; 67: 717-26
25. Sancar A, Lindsey-Boltz LA, Unsal-Kaccmaz K, Linn S. Molecular mechanism of mammalian DNA repair and the DNA damage checkpoints. Annu Rev Biochem 2004; 73: 39-85
26. Chinni SR, Li Y, Upadhyay S, Koppolu PK, Sarkar FH. Indole-3-carbinol (13C) induced cell growth inhibition, G1 cell cycle arrest and apoptosis in prostate cancer cells. Oncogene 2001; 20: 2927-36
27. Moragoda L, Jaszewski R, Majumdar AP. Curcumin induced modulation of cell cycle and apoptosis in gastric and colon cancer cells. Anticancer Res 2001; 21: 873-8