Rapid detection of lamivudine-resistant Hepatitis B virus mutations by PCR-based methods

Tohoku Journal of Experimental Medicine

Volumn 210, Issue 1, 2006, Pages 67-78

  Chieochansin, Thaweesak ^a   Chutinimitkul, Salin ^a   Payungporn, Sunchai ^a   Theamboonlers, Apiradee ^a   Tangkijvanich, Pisit ^a   Komolmit, Piyawat ^a   Poovorawan, Yong ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

Author keywords

HBV; LNA mediated TaqMan probes; PCR RFLP; Real time PCR; YMDD

Indexed keywords

DNA POLYMERASE; LAMIVUDINE; LOCKED NUCLEIC ACID; VIRUS DNA; VIRUS ENZYME;

ANTIBIOTIC RESISTANCE; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DNA DETERMINATION; DNA PROBE; GENE SEQUENCE; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PATIENT MONITORING; POLYMERASE CHAIN REACTION; PROTEIN MOTIF; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS MUTATION; WILD TYPE;

AMINO ACID MOTIFS; DNA MUTATIONAL ANALYSIS; DNA, VIRAL; DNA-DIRECTED DNA POLYMERASE; DRUG RESISTANCE, VIRAL; HEPATITIS B VIRUS; HEPATITIS B, CHRONIC; HUMANS; LAMIVUDINE; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION;

EID: 33748607451     PISSN: 00408727     EISSN: 13493329     Source Type: Journal    
DOI: 10.1620/tjem.210.67     Document Type: Article

References (27)

1. Aberle, S.W., Kletzmayr, J., Watschinger, B., Schmied, B., Vetter, N. & Puchhammer-Stockl, E. (2001) Comparison of sequence analysis and the INNO-LiPA HBV DR line probe assay for detection of lamivudine-resistant hepatitis B virus strains in patients under various clinical conditions. J. Clin. Microbiol., 39, 1972-1974.
2. Allen, M.I., Deslauriers, M., Andrews, C.W., Tipples, G.A., Walters, K.A., Tyrrell, D.L., National Brown for Lamivudine Clinical Investigation Group & Condreay, L.D. (1998) Identification and characterization of mutations in hepatitis B virus resistant to lamivudine. Hepatology., 27, 1670-1677.
3. Allen, M.I., Gauthier, J., DesLauriers, M., Bourne, E.J., Carrick, K.M., Baldanti, F., Ross, L.L., Lutz, M.W. & Condreay, L.D. (1999) Two sensitive PCR-based methods for detection of hepatitis B virus variants associated with reduced susceptibility to lamivudine. J. Clin. Microbiol., 37, 3338-3347.
4. Bowden, S., Bartholomeusz, A. & Locarnini, S. (2003) Lamivudine resistant occult HBV: implications for public health. J. Hepatol., 38, 526-528.
5. Braasch, D.A. & Corey, D.R. (2001) Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA. Chem. Biol., 8, 1-7.
6. Cane, P.A., Cook, P., Ratcliffe, D., Mutimer, D. & Pillay, D. (1999) Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus. Antimicrob. Agents. Chemother.,43, 1600-1608.
7. Choleva, Y., Norholm, M., Pedersen, S., Mouritzen, P., Hoiby, P.E., Nielsen, A.T., Moller, S., Jakobsen, M.H. & Kongsbak, L. (2001) Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics. JALA, 6, 92-97.
8. Das, K., Xiong, X., Yang, H., Westland, C.E., Gibbs, C.S., Sarafianos, S.G. & Arnold, E. (2001) Molecular modeling and biochemical characterization reveal the mechanism of hepatitis B virus polymerase resistance to lamivudine (3TC) and emtricitabine (FTC). J. Virol., 75, 4771-4779.
9. Hong, S.P., Kim, N.K., Hwang, S.G., Chung, H.J., Kim, S., Han, J.H., Kim, H.T., Rim, K.S., Kang, M.S., Yoo, W. & Kim, S.O. (2004) Detection of hepatitis B virus YMDD variants using mass spectrometric analysis of oligonucleotide fragments. J. Hepatol., 40, 837-844.
10. Ichikawa, E., Yamamura, S. & Kato, K. (1999) Synthesis of 2', 3'-dideoxy-3'3-c-(hydroxymethyl)-4'-thiopentofuranosyl nucleosides as potential antiviral agent. Bioorg. Med. Chem. Lett., 9, 1113-1114.
11. Inoue, J., Ueno, Y., Kanno, N., Anzai, H., Kondo, Y., Moritoki, Y., Mikami, E., Chiba, M., Kogure, T., Nagasaki, F., Fukushima, K., Iwasaki, T., Satomi, S. & Shimosegawa, T. (2005) Living related liver transplantation for acute fulminant hepatitis B: experience from two possible hyper-acute cases. Tohoku J. Exp. Med., 205, 197-204.
12. Jang, H., Cho, M., Heo, J., Kim, H., Jun, H., Shin, W., Cho, B., Park, H. & Kim, C. (2004) Oligonucleotide chip for detection of Lamivudine-resistant hepatitis B virus. J. Clin. Microbiol., 42, 4181-4188.
13. Jardi, R., Buti, M., Rodriguez-Frias, F., Cotrina, M., Costa, X., Pascual, C., Esteban, R. & Guardia, J. (1999) Rapid detection of lamivudine-resistant hepatitis B virus polymerase gene variants. J. Virol. Methods., 83, 181-187.
14. Johnson, M.P., Haupt, L.M. & Griffiths, L.R. (2004) Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR. Nucleic. Acids. Res., 32, e55.
15. Kennedy, B., Arar, K., Reja, V. & Henry, R.J. (2005) Locked nucleic acids for optimizing displacement probes for quantitative real-time PCR. Anal. Biochem., 348, 294-299.
16. Kirishima, T., Okanoue, T., Daimon, Y., Itoh, Y., Nakamura, H., Morita, A., Toyama, T. & Minami, M. (2002) Detection of YMDD mutant using a novel sensitive method in chronic liver disease type B patients before and during lamivudine treatment. Anal. Biochem., 37, 259-265.
17. Koshkin, A.A., Rajwanshi, V.K. & Wengel, J. (1998) Novel convenient syntheses of LNA [2.2.1]bicyclo nucleosides. Tetrahedron. Letters., 39, 4381-4384.
18. Letertre, C., Perelle, S., Dilasser, F., Arar, K. & Fach, P. (2003) Evaluation of the performance of LNA and MGB probes in 5'-nuclease PCR assays. Mol. Cell. Probes., 17, 307-311.
19. Papatheodoridis, G.V., Dimou, E. & Papadimitropoulos, V. (2002) Nucleoside analogues for chronic hepatitis B: antiviral efficacy and viral resistance. Am. J. Gastroenterol., 97, 1618-1628.
20. Pei, F., Ning, J.Y., You, J.F., Yang, J.P. & Zheng, J. (2005) YMDD variants of HBV DNA polymerase gene: rapid detection and clinicopathological analysis with long-term lamivudine therapy after liver transplantation. World. J. Gastroenterol., 14, 2714-2719.
21. Sablon, E. & Shapiro, F. (2005) Advances in Molecular Diagnosis of HBV Infection and Drug Resistance. Int. J. Med. Sci., 2, 8-16.
22. Seeger, C. & Mason, W.S. (2000) Hepatitis B virus biology. Microbiol. Mol. Biol. Rev., 64, 51-68.
23. Stuyver, L., Geyt, C.V., Gendt, S.D., Reybroeck, G.V., Zoulim, F., Roels, G.L. & Rossau, R. (2000) Line probe assay for monitoring drug resistance in hepatitis B virus-infected patients during antiviral therapy. J. Clin. Microbiol., 38, 702-707.
24. Stuyver, L.J., Locarnini, S.A., Lok, A., Richman, D.D., Carman, W.F., Dienstag, J.L., Schnazi, R.F. & The HEP DART inthenational committee (2001) Nomenclature for antiviral-resistant human hepatitis B virus mutation in the polymerase region. Hepatology, 33, 751-757.
25. Suzuki, N., Yoshida, A. & Nakano, Y. (2005) Quantitative analysis of multi-species oral biofilms by TaqMan Real-Time PCR. Clin. Med. Res., 3, 176-185.
26. Terui, Y., Saito, T., Watanabe, H., Aoki, M., Haga, H., Miyano, S., Takeda, T., Togashi, H., Shinzawa, H. & Takahashi, T. (2000) Lamivudine as alternative therapy for interferon-resistant chronic hepatitis B and the characteristics of hepatitis B virus: a case report. Tohoku J. Exp. Med., 191, 247-253.
27. Wang, G., Gunic, E., Girardet, J.L. & Stoisavljevic, V. (1999) Conformationally locked nucleosides. Synthesis and hybridization properties of oligodeoxynucleotides containing 2',4'-C-bridged 2'-deoxynucleosides. Bioorg. Med. Chem. Lett., 19, 1147-1150.