Detection of clonally related vanB2-containing Enterococcus faecium strains in two Spanish hospitals

Journal of Medical Microbiology

Volumn 55, Issue 9, 2006, Pages 1237-1243

  Torres, Carmen ^a   Escobar, Susanna ^b   Portillo, Aránzazu ^a,c   Torres, Luis ^b   Rezusta, Antonio ^b   Ruiz Larrea, Fernanda ^a   Revillo, Maria José ^b   Aspiroz, Carmen ^d   Zarazaga, Myriam ^a  

^a UNIVERSITY OF LA RIOJA   (Spain)

^b HOSPITAL UNIVERSITARIO MIGUEL SERVET   (Spain)

^c Hospital de La Rioja   (Spain)

^d HOSPITAL ROYO VILLANOVA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOGLYCOSIDE ANTIBIOTIC AGENT; AMPICILLIN; CEFEPIME; CEFTAZIDIME; CIPROFLOXACIN; ERYTHROMYCIN; IMIPENEM; KANAMYCIN; SPACER DNA; STREPTOMYCIN; TEICOPLANIN; TETRACYCLINE; VANCOMYCIN;

AMINO ACID SUBSTITUTION; ANT(6') IA GENE; ANTIBIOTIC RESISTANCE; APH(3') IIIA GENE; ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM DETECTION; ENTEROCOCCUS FAECIUM; ERM(B) GENE; ESP GENE; GENE CLUSTER; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; SPAIN; TRANSPOSON; VANB2 GENE; VANCOMYCIN RESISTANT ENTEROCOCCUS; VANX B GENE;

ANTI-BACTERIAL AGENTS; CROSS INFECTION; DNA FINGERPRINTING; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DNA, INTERGENIC; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECIUM; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPAIN; VANCOMYCIN RESISTANCE;

EID: 33748568809     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.46560-0     Document Type: Article

References (38)

1. Aarestrup, F. M., Agerso, Y., Gerner-Smidt, P., Madsen, M. & Jensen, L. B. (2000). Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark. Diagn Microbiol Infect Dis 37, 127-137.
2. Carias, L. L., Rudin, S. D., Donskey, C. J. & Rice, L. B. (1998). Genetic linkage and cotransfer of a novel vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate. J Bacteriol 180, 4426-4434.
3. Cetinkaya, Y., Falk, P. & Mayhall, C. G. (2000). Vancomycin-resistant enterococci. Clin Microbiol Rev 13, 686-707.
4. Clark, N. C., Olsvik, Ø., Swenson, J. M., Spiegel, C. A. & Tenover, F. C. (1999). Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis. Antimicrob Agents Chemother 43, 157-160.
5. Dahl, K. H., Simonsen, G. S., Olsvik, Ø. & Sundsfjord, A. (1999). Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci. Antimicrob Agents Chemother 43, 1105-1110.
6. Dahl, K. H., Lundblad, E. W., Rokenes, T. P., Olsvik, Ø. & Sundsfjord, A. (2000). Genetic linkage of the vanB2 gene cluster to Tn5382 in vancomycin-resistant enterococci and characterization of two novel insertion sequences. Microbiology 146, 1469-1479.
7. Domingo, M.-C., Huletsky, A., Bernal, A., Giroux, R., Boudreau, D. K., Picard, F. J. & Bergeron, M. G. (2005). Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces. J Antimicrob Chemother 55, 466-474.
8. Dunny, G. M., Craig, R. A., Carron, R. L. & Clewell, D. B. (1979). Plasmid transfer in Streptococcus faecalis: production of multiple sex pheromones by recipients. Plasmid 2, 454-465.
9. Dutka-Malen, S., Evers, S. & Courvalin, P. (1995). Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33, 24-27.
10. Garnier, F., Taourit, S., Glaser, P., Courvalin, P. & Galimand, M. (2000). Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp. Microbiology 146, 1481-1489.
11. Gholizadeh, Y. & Courvalin, P. (2000). Acquired and intrinsic glycopeptide resistance in enterococci. Int J Antimicrob Agents 16, S11-S17.
12. Gold, H. S., Ünal, S., Cercenado, E., Thauvin-Eliopoulos, C., Eliopoulos, G. M., Wennersten, C. B. & Moellering, R. C., Jr (1993). A gene conferring resistance to vancomycin but not teicoplanin in isolates of Enterococcus faecalis and Enterococcus faecium demonstrates homology with vanB, vanA, and vanC genes of enterococci. Antimicrob Agents Chemother 37, 1604-1609.
13. Granlund, M., Carlsson, C., Edebro, H., Emanuelsson, K. & Lundholm, R. (2006). Nosocomial outbreak of vanB2 vancomycin-resistant Enterococcus faecium in Sweden. J Hosp Infect 62, 254-256.
14. Jureen, R., Top, J., Mohn, S. C., Harthug, S., Langeland, N. & Willens, R. J. L. (2003). Molecular characterization of ampicillin-resistant Enterococcus faecium isolates from hospitalized patients in Norway. J Clin Microbiol 41, 2330-2336.
15. Kawalec, M., Gniadkowski, M., Zielinska, U., Klos, W. & Hryniewicz, W. (2001). Vancomycin-resistant Enterococcus faecium strain carrying the vanB2 gene variant in a Polish hospital. J Clin Microbiol 39, 811-815.
16. Klare, I., Konstabel, C., Mueller-Bertling, S. & 10 other authors (2005). Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals. Eur J Clin Microbiol Infect Dis 24, 815-825.
17. Lee, W. G., Jernigan, J. A., Rashed, J. K., Anderson, G. J. & Tenover, F. C. (2001). Possible horizontal transfer of the vanB2 gene among genetically diverse strains of vancomycin-resistant Enterococcus faecium in a Korean hospital. J Clin Microbiol 39, 1165-1168.
18. Lorenzo-Diaz, F., Delgado, T., Reyes-Darias, J. A., Flores, C., Méndez-Álvarez, S., Villar, J., Sierra, A. & Claverie-Martín, F. (2004). Characterization of the first VanB vancomycin-resistant Enterococcus faecium isolated in a Spanish hospital. Curr Microbiol 48, 199-203.
19. Lu, J.-J., Perng, C.-L., Ho, M.-F., Chiueh, T.-S. & Lee, W.-H. (2001). High prevalence of VanB2 vancomycin-resistant Enterococcus faecium in Taiwan. J Clin Microbiol 39, 2140-2145.
20. Lu, J.-J., Chang, T.-Y., Perng, C.-L. & Lee, S.-Y. (2005). The vanB2 gene cluster of the majority of vancomycin-resistant Enteroccus faecium isolates from Taiwan is associated with the pbp5 gene and is carried by Tn5382 containing a novel insertion sequence. Antimicrob Agents Chemother 49, 3937-3939.
21. Mato, R., de Lencastre, H., Roberts, R. B. & Tomasz, A. (1996). Multiplicity of genetic backgrounds among vancomycin-resistant Enterococcus faecium isolates recovered from an outbreak in a New York City hospital. Microb Drug Resist 2, 309-317.
22. McGregor, K. F. & Young, H.-K. (2000). Identification and characterization of vanB2 glycopeptide resistance elements in enterococci isolated in Scotland. Antimicrob Agents Chemother 44, 2341-2348.
23. McGregor, K. F., Nolan, C., Young, H.-K., Palepou, M.-F. I., Tysall, L. & Woodford, N. (2001). Prevalence of the vanB2 gene cluster in the VanB glycopeptide-resistant enterococci in the United Kingdom and the Republic of Ireland and its association with a Tn5382-like element. Antimicrob Agents Chemother 45, 367-368.
24. Miele, A., Bandera, M. & Goldstein, B. P. (1995). Use of primers selective for vancomycin resistance genes to determine van genotype in enterococci and to study gene organization in VanA isolates. Antimicrob Agents Chemother 39, 1772-1778.
25. Murray, B. (1997). Vancomycin-resistant enterococci. Am J Med 102, 284-293.
26. NCCLS (2002). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. NCCLS document M7-A5. Wayne, PA: National Committee for Clinical Laboratory Standards.
27. Perichon, B., Reynolds, P. & Courvalin, P. (1997). VanD-type glycopeptide-resistant Enterococcus faecium BM4339. Antimicrob Agents Chemother 41, 2016-2018.
28. Quintiliani, R., Jr, Evers, S. & Courvalin, P. (1993). The vanB gene confers various levels of self-transferable resistance to vancomycin in enterococci. J Infect Dis 167, 1220-1223.
29. Rice, L. B., Bellais, S., Carias, L. L., Mutton-Thomas, R., Bonomo, R. A., Caspers, P., Page, M. G. P. & Gutmann, L. (2004). Impact of specific pbp5 mutations on expression of β-lactam resistance in Enterococcus faecium. Antimicrob Agents Chemother 48, 3028-3032.
30. Simonsen, G. S., Andersen, B. M., Digranes, A. & 9 other authors (1998). Low faecal carrier rate of vancomycin resistant enterococci in Norwegian hospital patients. Scand J Infect Dis 30, 465-468.
31. Sutcliffe, J., Grebe, T., Tait-Kamradt, A. & Wondrack, L. (1996). Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother 40, 2562-2566.
32. Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H. & Swaminathan, B. (1995). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33, 2233-2239.
33. Torres, C., Reguera, J. A., Sanmartin, M. J., Pérez-Díaz, J. C. & Baquero, F. (1994). vanA-mediated vancomycin-resistant Enterococcus spp. in sewage. J Antimicrob Chemother 33, 553-561.
34. Torres, C., Tenorio, C., Portillo, A., García, M., Martínez, C., del Campo, R., Ruiz-Larrea, F. & Zarazaga, M. (2003). Intestinal colonization by vanA- or vanB2-containing enterococcal isolates of healthy animals in Spain. Microb Drug Resist 9 (Suppl. 1), S47-S52.
35. Turabelidze, D., Kotetishvili, M., Krefer, A., Morris, J. G. & Sulakvelidze, A. (2000). Improved pulsed-field gel electrophoresis for typing vancomycin-resistant enterococci. J Clin Microbiol 38, 4242-4245.
36. Umeda, A., Garnier, F., Courvalin, P. & Galimand, M. (2002). Association between the vanB2 glycopeptide resistance operon and Tn1549 in enterococci from France. J Antimicrob Chemother 50, 253-256.
37. Van de Klundert, J. A. M. & Vliegenthart, J. S. (1993). PCR detection of genes for aminoglycoside-modifying enzymes. In Diagnostic Molecular Microbiology: Principles and Applications, pp. 547-552. Edited by D. H. Persing, T. F. Smith, F. C. Tenover & T. J. White. Washington, DC: American Society for Microbiology.
38. Woodford, N. (2001). Epidemiology of the genetic elements responsible for acquired glycopeptide resistance in enterococci. Microb Drug Resist 7, 229-236.