Characterization of the First VanB vancomycin-resistant Enterococcus faecium isolated in a Spanish Hospital

Current Microbiology

Volumn 48, Issue 3, 2004, Pages 199-203

  Lorenzo Díaz, Fabián ^a   Delgado, Teresa ^b   Reyes Darias, José A ^a,d   Flores, Carlos ^a   Méndez Alvarez, Sebastián ^a,c   Villar, Jesús ^a   Sierra, Antonio ^b   Claverie Martín, Félix ^a  

^a HOSPITAL UNIVERSITARIO NUESTRA SEÑORA DE CANDELARIA   (Spain)

^b HOSPITAL UNIVERSITARIO DE CANARIAS   (Spain)

^c UNIVERSIDAD DE LA LAGUNA   (Spain)

^d University of Georgia ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VANCOMYCIN; ANTIINFECTIVE AGENT; BACTERIAL DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; GENE CLUSTER; GENE SEQUENCE; HOSPITAL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPAIN; TRANSPOSON; AGED; BACTERIAL INFECTION; CHEMISTRY; DECUBITUS; DNA SEQUENCE; DRUG EFFECT; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MOLECULAR GENETICS; PHYSIOLOGY; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

ENTEROCOCCUS FAECIUM;

AGED; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ENTEROCOCCUS FAECIUM; GENES, BACTERIAL; GRAM-POSITIVE BACTERIAL INFECTIONS; HOSPITALS; HUMANS; MALE; MOLECULAR SEQUENCE DATA; PRESSURE ULCER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY; SPAIN; VANCOMYCIN; VANCOMYCIN RESISTANCE;

EID: 2942629357     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-003-4125-2     Document Type: Article

References (24)

1. Arthur M, Molinas C, Depardieu F, Courvalin P (1993) Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147. J Bacteriol 175:117-127
2. Carias LL, Rudin SD, Donskey CJ, Rice LB (1998) Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate. J Bacteriol 180:4426-4434.
3. Cetinkaya Y, Pamela F, Mayhall CG (2000) Vancomycin-resistant Enterococci. Clin Microbiol Rev 13:686-707
4. Cisterna R, Ibarra K, Morla A, Basaras M, Cisterna C, Herreras A, Borja J, Grupo Español de Estudio y Vigilancia de Resistencias (1999) Multicenter study of resistance in enterococci. The role of teicoplanin. Rev Esp Quimioterap 12:237-243
5. Dahl KH, Simonsen GS, Olsvik O, Sundsfjord A (1999). Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci. Antimicrob Agents Chemother 43:1105-1110
6. Del Campo R, Tenorio C, Jiménez-Diaz R, Rubio C, Gomez-Lus R, Baquero F, Torres C (2001) Bacteriocin production in vancomycin-resistant and vancomycin-susceptible Enterococcus isolates of different origins. Antimicrob Agents Chemother 45:905-912
7. Dutka-Malen S, Evers S, Courvalin P (1995) Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33: 24-27
8. B two component regulatory system in Enterococcus faecalis V583. J Bacteriol 178: 1302-1309
9. Evers S, Reynolds PE, Courvalin P (1994) Sequence of the vanB and ddl genes encoding D-alanine: D-lactate and D-alanine: D-alanine ligases in vancomycin-resistant Enterococcus faecalis V583. Gene 140:97-102
10. Garnier F, Taourit S, Glaser P, Courvalin P, Galimand M (2000) Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp. Microbiology 146:1481-1489
11. Gold HS, Unal S, Cercenado E, Thauvin-Eliopoulos C, Eliopoulos GM, Wennersten CB, Moellering JrRC (1993) A gene conferring resistance to vancomycin but not teicoplanin in isolates of Enterococcus faecalis and Enterococcus faecium demonstrates homology with vanB, vanA, and vanC genes of enterococci. Antimicrob Agents Chemother 37:1604-1609
12. Kee D, Picard FJ, Martineau F, Menard C, Roy PH, Ouellette M, Bergeron MG (1999) Development of a PCR assay for rapid detection of enterococci. J Clin Microbiol 37:3497-3503
13. Lee WG, Jernigan JA, Rasheed JK, Anderson GJ, Tenover FC (2001) Possible horizontal transfer of the vanB2 gene among genetically diverse strains of vancomycin-resistant Enterococcus faecium in a Korean hospital. J Clin Microbiol 39:1165-1168
14. Lu JJ, Perng CL, Ho MF, Chiueh TS, Lee WH (2001) High prevalence of VanB2 vancomycin-resistant Enterococcus faecium in Taiwan. J Clin Microbiol 39:2140-2145
15. McGregor KF, Young HK (2000) Identification and characterization of vanB2 glycopeptide resistance elements in Enterococci isolated in Scotland. Antimicrob Agents Chemother 44:2341-2348
16. McGregor KF, Nolan C, Young HK, Palepou MF, Tysall L, Woodford N (2001) Prevalence of the vanB2 gene cluster in VanB glycopeptide-resistant enterococci in the United Kingdom and the Republic of Ireland and its association with a Tn5382-like element. Antimicrob Agents Chemother 45:367-368
17. McKessar SJ, Berry AM, Bell JM, Turnidge JD, Paton JC (2000) Genetic characterization of vanG, a novel vancomycin resistance locus of Enterococcus faecalis. Antimicrob Agents Chemother 44:3224-3228
18. Méndez-Álvarez S, Pérez-Hernández X, Claverie-Martin F (2000) Glycopeptide resistance in enterococci. Int Microbiol 3:71-80
19. National Committee for Clinical Laboratory Standards (2000) Methods for dilution antimicrobal susceptibility test for bacteria that grow aerobically, 5th ed. Approved standard M7-A5. National Committee for Clinical Laboratory Standards, Waine, PA
20. Patel R, Uhl JR, Kohner P, Hopkins MK, Cockerill III FR (1997) Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci. J Clin Microbiol 35:703-707
21. Patel R, Uhl JR, Kohner P, Hopkins MK, Steckelberg JM, Kline B, Cockerill III FR (1998) DNA sequence variation within vanA, vanB, vanC-1, and vanC-2/3 genes of clinical Enterococcus isolates. Antimicrob Agents Chemother 42:202-205
22. Pérez-Roth E, Claverie-Martin F, Villar J, Méndez- Álvarez S (2001) Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J Clin Microbiol 39:4037-4041
23. Pérez-Hernández X, Méndez-Álvarez S, Claverie-Martin F (2002) A PCR assay for rapid detection of vancomycin resistant enterococci. Diag Microbiol Infect Dis 42:273-277
24. Quintiliani R Jr, Courvalin P (1996) Characterization of Tn1547, a composite transposon flanked by the IS16 and IS256-like elements, that confers vancomycin resistance in Enterococcus faecalis BM4281. Gene 172:1-8