A new colorimetric assay for methionyl aminopeptidases: Examination of the binding of a new class of pseudopeptide analog inhibitors

Analytical Biochemistry

Volumn 357, Issue 1, 2006, Pages 43-49

  Mitra, Sanghamitra ^a   Dygas Holz, Anna M ^a   Jiracek, Jiri ^b   Zertova, Miroslava ^b   Zakova, Lenka ^b   Holz, Richard C ^a,c  

^a UTAH STATE UNIVERSITY   (United States)

^b INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^c LOYOLA UNIVERSITY CHICAGO   (United States)

Author keywords

Enzyme inhibition; Methionine aminopeptidases; Spectrophotometric assay

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY; TEMPERATURE DISTRIBUTION;

COLORIMETRIC ASSAYS; KINETIC CONSTANT; METHIONINE AMINOPEPTIDASES; METHIONYL AMINOPEPTIDASES; PYROCOCCUS FURIOSUS; SPECTROPHOTOMETRIC ASSAYS; TEMPERATURE DEPENDENCE; UV-VIS SPECTROPHOTOMETERS;

ENZYME INHIBITION;

CHROMOGENIC SUBSTRATE; METHIONYL AMINOPEPTIDASE; PSEUDOPEPTIDE;

ANALYTIC METHOD; ANGIOGENESIS; ARTICLE; BINDING AFFINITY; COLORIMETRY; ENZYME ASSAY; ENZYME SUBSTRATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; PRIORITY JOURNAL; PYROCOCCUS FURIOSUS; SPECTROPHOTOMETRY; TEMPERATURE DEPENDENCE;

ESCHERICHIA COLI; PYROCOCCUS FURIOSUS;

EID: 33748329839     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.06.012     Document Type: Article

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