New protein extraction/solubilization protocol for gel-based proteomics of rat (female) whole brain and brain regions

Molecules and Cells

Volumn 22, Issue 1, 2006, Pages 119-125

  Hirano, Misato ^a   Rakwal, Randeep ^a   Shibato, Junko ^a   Agrawal, Ganesh Kumar ^b,c   Jwa, Nam Soo ^d   Iwahashi, Hitoshi ^a   Masuo, Yoshinori ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b Research Laboratory for Agricultural Biotechnology and Biochemistry Nepal   (Nepal)

^c University of Missouri   (United States)

^d Sejong University   (South Korea)

Author keywords

Gel based proteomics; IPG; Lysis buffer; Peptide mass fingerprinting; Rat brain; TCA acetone

Indexed keywords

ACETONE; BUFFER; DYE; MEMBRANE PROTEIN; PHOSPHOPROTEIN; PROTEOME; SILVER NITRATE; TRICHLOROACETIC ACID;

ANIMAL TISSUE; ARTICLE; BRAIN REGION; CONTROLLED STUDY; FEMALE; HYPOPHYSIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PH; PRECIPITATION; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; PROTEOMICS; RAT; SOLUBILIZATION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; BRAIN CHEMISTRY; CHEMICAL FRACTIONATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FEMALE; NERVE TISSUE PROTEINS; PROTEOMICS; RATS; RATS, WISTAR; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 33748306112     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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