Photolabile peptide nucleic acid monomers: Synthesis and photodeprotection

Synlett

Volumn , Issue 13, 2006, Pages 2130-2132

  Bhushan, Kumar R ^a  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

DNA; Genomics; Photolabile; Protecting groups; Solid phase synthesis

Indexed keywords

MONOMER; PEPTIDE NUCLEIC ACID;

ARTICLE; DEPROTECTION REACTION; SOLID; SYNTHESIS;

EID: 33748285790     PISSN: 09365214     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2006-949621     Document Type: Article

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26. In a typical procedure to attach photolabile PNA to an amino-glass slide, NPPOC-T was attached to the ammoglass slide mounted in a flow cell by activation of the carboxyl groups of NPPOC-T (14.7 mg, 0.03 mmol) in DMF (0.25 mL). After 20 min, excess NPPOC-T was flushed out with MeCN and specific areas (pixels) were irradiated at 365 nm in MeCN using DMD for varying period of time to remove the photolabile protecting groups at specific locations. The amino groups exposed by this treatment were visualized by being covalently coupled to a BODIPY dye (100 μg) in DMF (0.25 mL) for 10 min followed by scanning on a GenPix Scanner.
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