Metallothionein, antioxidant enzymes and DNA strand breaks as biomarkers of Cd exposure in a marine crab, Charybdis japonica

Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

Volumn 144, Issue 1, 2006, Pages 67-75

  Pan, Luqing ^a   Zhang, Hongxia ^a  

^a Ocean University of China   (China)

Author keywords

Antioxidant enzyme; Biomarkers; Cadmium; Charybdis japonica; Crab; DNA strand break; Metallothionein (MT); Oxidative stress

Indexed keywords

ANTIOXIDANT; BIOLOGICAL MARKER; CADMIUM; CATALASE; DNA; ENZYME; GLUTATHIONE PEROXIDASE; METALLOTHIONEIN; SUPEROXIDE DISMUTASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOLOGICAL MONITORING; CHARYBDIS JAPONICA; CONTROLLED STUDY; CRAB; DETOXIFICATION; DNA STRAND BREAKAGE; ENZYME ACTIVATION; ENZYME ACTIVITY; GILL; HEPATOPANCREAS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; SENSITIVITY ANALYSIS;

CHARYBDIS JAPONICA; DECAPODA (CRUSTACEA); PORTUNIDAE;

EID: 33748070791     PISSN: 15320456     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpc.2006.06.001     Document Type: Article

References (69)

1. Almeida J.A., Diniz Y.S., Marques S.F.G., Faine L.A., Ribas B.O., Burneiko R.C., and Novelli E.L.B. The use of the oxidative stress responses as biomarkers in Nile tilapia (Oreochromis niloticus) exposed to in vivo Cd contamination. Environ. Int. 27 (2002) 673-679
2. Amiard J.C., and Cosson R.P. Les métallothionéines. In: Lagadic L., Caquet T., Amiard J.C., and Ramade F. (Eds). Biomarqueurs en écotoxicologie: Aspects Fondamentaux, Masson (1997), Masson, Paris 53-66
3. Baudriment M., Mettvaud J., Ribeyre R.M., and Boudou A. Bioaccumulation and metallothionein in the Asiatic clam (Corbicula fluminea) after experimental exposure to cadmium and inorganic mercury. Environ. Toxicol. Chem. 16 (1997) 2096-2105
4. Bem E.M., Mailer K., and Elson C.M. Influence of mercury (II),cadmium (II), methylmercury (II), and phenylmercury on the kinetic properties of rat liver glutathione peroxidase. Can. J. Biochem. Cell Biol. 63 (1985) 1212-1216
5. Black M.C., Ferrell J.R., Horning R.C., and Martin Jr. L.K. DNA strand breakage in freshwater mussels (Anodonta grandis) exposed to lead in the laboratory and field. Environ. Toxicol. Chem. 15 (1996) 802-806
6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72 (1976) 248-254
7. Brouwer M., and Brouwer T.H. Biochemical defense mechanisms against copper-induced oxidative damage in the blue crab, Callinectes sapidus. Arch. Biochem. Biophys. 351 (1998) 257-264
8. Cesarone D.F., Bologenesi C., and Santi L. Improved microfluorometric DNA determination in biological material using 33258 Hoechst. Anal. Biochem. 100 (1979) 188-197
9. Chapman P.M. Sediment quality assessment: status and outlook. J. Aquat. Ecosyst. Health 4 (1995) 183-194
10. Chandra P., and Khuda-Bukhsh A.R. An assay of genotoxicity produced by cadmium chloride in fish (Oreochromis mossambicus) and efficacy of vitamin C in its alterations. In: Manna G.K., and Roy S.C. (Eds). Perspectives in Cytology and Genetics vol. 10 (2001), AICCG Publ., Kalyani, India 583-590
11. Chandra P., and Khuda-Bukhsh A.R. Genotoxic effects of cadmium chloride and azadirachtin treated singly and in combination in fish. Ecotoxicol. Environ. Saf. 58 (2004) 194-201
12. Chen J.L., Liu W.X., Liu S.Z., Lin X.M., and Tao S. An evaluation on heavy metal contamination in the surface sediments in Bohai Sea. Mar. Sci. 28 (2004) 16-21
13. Ching E.W.K., Siu W.H.L., Lam P.K.S., Xu L., Zhang Y., Richardson B.J., and Wu R.S.S. DNA adduct formation and DNA strand breaks in green-lipped mussels (Perna viridis) exposed to benzo[a]pyrene: dose-and time-dependent relationships. Mar. Pollut. Bull. 42 (2001) 603-610
14. Company R., Serafim A., Bebianno M.J., Cosson R., Shillito B., and Fiala-Médioni A. Effect of cadmium, copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus. Mar. Environ. Res. 58 (2004) 377-381
15. Company R., Serafim A., Cosson R., Camus L., and Shilito B. The effect of cadmium on antioxidant responses and the susceptibility to oxidative stress in the hydrothermal vent mussel Bathymodiolus azoricus. Mar. Biol. 148 (2006) 817-825
16. Cossu C., Doyotte A., Jacquin M.C., Babut M., Exinger A., and Vasseur P. Glutathione reductase, selenium-dependent glutathione peroxidase, glutathione levels, and lipid peroxidation in freshwater bivalves, Unio tumidus, as biomarkers of aquatic contamination in field studies. Ecotoxicol. Environ. Saf. 38 (1997) 122-131
17. Daniel F.B., Haas D.L., and Santi L. Quantitation of chemically induced DNA strand breaks in human cells via an alkaline unwinding assay. Anal. Biochem. 144 (1985) 390-402
18. Del Ramo J., Torreblanca A., Martínez M., Pastor A., and Díaz-Mayans J. Quantification of cadmium-induced metallothionein in crustaceans by the silver-saturation method. Mar. Environ. Res. 39 (1995) 121-125
19. Dutton M.D., Stephenson M., and Klaverkamp J.F. A. Mercury saturation assay for measuring metallothionein in fish. Environ. Toxicol. Chem. 12 (1993) 1193-1202
20. Engel D.W., and Brouwer M. Crustacean as models of metal metabolism: I. Effects of the molt cycle on blue crab metal metabolism and metallothionein. Mar. Environ. Res. Res.35 (1993) 1-5
21. Engleken J., and Hildebrandt A. cDNA cloning and cadmium-induced expression of metallothionein mRNA in the zebra mussel Dreissena polymorpha. Biochem. Cell. Biol.77 (1999) 237-241
22. Everaarts J.M., and Sarkar A. DNA damage as a biomarker of marine pollution: strand breaks in seastars (Asterias rubens) from the north sea. Water Sci. Technol. 34 (1996) 157-162
23. Faucher K., Fichet D., Miramand P., and Lagardère J.P. Impact of acute cadmium exposure on the trunk lateral line neuromasts and consequences on the "C-start" response behaviour of the sea bass (Dicentrarchus labrax L.; Teleostei, Moronidae). Aquat. Toxicol. 76 (2006) 278-294
24. George S.G., Hodgson P., Todd K., and Tyter P. Metallothionein protects against cadmium toxicity-proof from studies developing turbot larvae. Mar. Environ. Res. 42 (1996) 1-4
25. Geret F., Serafim A., Barreira L., and Bebianno M.J. Response of antioxidant systems to copper in the gills of the clam Ruditapes decussates. Mar. Environ. Res. 54 (2002) 413-417
26. Giamberini L., and Cajaraville M.P. Lysosomal responses in the digestive gland of the freshwater mussel, Dreissena polymorpha, experimentally exposed to cadmium. Environ. Res. 98 (2005) 210-214
27. Greanwald R.A. Handbook of Methods for Oxygen Radical Research (1985), CRC Press, Boca Raton, FL
28. Hamza-Chaffai A., Aniard J.C., and Cosson R.P. Relationship between metallothionein and metals in a natural population of the clam Ruditapes decussates from Sfax coast: a non-linear model using box-cox transformation. Comp. Biochem. Physiol., C 123 (1999) 153-163
29. Hanas J.S., and Gunn C.G. Inhibition of transcription. factor IIIA-DNA interactions by xenobiotic metal ions. Nucleic Acids Res. 24 (1996) 924-930
30. Hook S.E., and Lee R.F. Interactive effects of UV, benzo[a] pyrene, and cadmium on DNA damage and repair in embryos of the grass shrimp Palaemonetes pugio. Mar. Environ. Res. 58 (2004) 735-739
31. Klaverkamp J.F., Wautier K., and Baron C.L. A modified mercury saturation assay for measuring metallothionein. Aquat. Toxicol. 50 (2000) 13-25
32. Kono Y., and Fridovich I. Superoxide radicals inhibit catalase. J. Biol. Chem. 257 (1982) 5751-5754
33. Lange A., Ausseil O., and Segner H. Alterations of tissue glutathione levels and metallothionein mRNA in rainbow trout during single and combined exposure to cadmium and zinc. Comp. Biochem. Physiol., C 131 (2002) 231-243
34. Lecoeur S., Videmann B., and Berny P. Evaluation of metallothionein as a biomarker of single and combined Cd/Cu exposure in Dreissena polymorpha. Environ. Res. 94 (2004) 184-191
35. Legras S., Mouneyrac C., Amiard J.C., Amiard-Triquet C., and Rainbow P.S. Changes in metallothionein concentrations in response to variation in natural factors (salinity, sex, weight) and metal contamination in crabs from a metal-rich estuary. J. Exp. Mar. Biol. Ecol. 246 (2000) 259-279
36. Li S.M., Hu D.Y., and Tang C.S. Biological effect of metallothionein. Foreign Med. Sci. (Sec. Pathophysiol. Clin. Med.) 16 (1996) 36-38
37. Martínez M., Del Ramo J., Torreblanca A., and Díaz-Mayans J. Cadmium toxicity accumulation and metallothionein induction in Echinogammarus echinosetosus. J. Environ. Sci. Health. Part A, Environ. Sci. Eng. Toxic Hazard. Substance Control A31 (1996) 1605-1617
38. Martínez M., Del Ramo J., Torreblanca A., and Díaz-Mayans J. Effect of cadmium exposure on zinc levels in the brine shrimp Artemia parthenogenetica. Aquaculture 172 (1999) 315-325
39. Marklund S., and Marklund G. Involvement of the superoxide anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47 (1974) 169-479
40. McCord L.M., and Fridovich I. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244 (1969) 6049-6055
41. Moksnes P., Lindahl U., and Haux C. Metallothionein as a bioindicator of heavy metal exposure in the tropical shrimp, Penaeus vannamei: a study of dose-dependent induction. Mar. Environ. Res. 39 (1995) 143-146
42. Mouneyrac C., Amiard-Triquet C., Amiard J.C., and Rainbow P.S. Comparison of metallothionein concentrations and tissue distribution of trace metals in crabs (Pachygrapsus marmoratus) from a metal-rich estuary, in and out of the reproductive season. Comp. Biochem. Physiol., C 129 (2001) 193-209
43. Novelli E.L.B., Vieir E.P., Rodrigues N.L., and Ribas B.O. Risk assessment of cadmium toxicity on hepatic and renal tissues of rats. Environ. Res. 79 (1998) 102-105
44. Olive P.L. DNA damage and repair in inidividual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol. 75 (1999) 395-405
45. Omaye S.T., and Tappel A.L. Effect of cadmium chloride on the rat testicular soluble seleno-enzyme glutathione peroxidase. Res. Commun. Chem. Pathol. Pharmacol. 12 (1975) 695-711
46. Pan L.Q., Ren J.Y., and Wu Z.W. Effects of heavy metal ions on SOD, CAT activities of hepatopancreas and gill of the crab Eriocheir sinensis. J. Ocean. Univ. Chin. 34 (2004) 189-194
47. Pedersen K.L., Pedersen S.N., and Hojrup P. Purification and characterization of a cadmium-induced metallonthionein from the shore crab Carcinus maenas (L). Biochem. J. 297 (1994) 609-614
48. Pedersen S.N., Lundebye A.K., and Depledge M.H. Field application of metallothionein and stress protein biomarkers in the shore crab (Carcinus maenas) exposed to trace metals. Aquat. Toxicol. 37 (1997) 183-200
49. Power E.A., and Chapman P.M. Assessing sediment quality [A].Sediment toxicity assessment [C] (1992), Lewis Pub, Boca Raton 1-18
50. Pruski A.M., and Dixon D.R. Effects of cadmium on nuclear integrity and DNA repair efficiency in the gill cells of Mytilus edulis L Aquat. Toxicology 57 (2002) 127-137
51. Rainbow P.S. Trace metal accumulation in marine invertebrates: marine biology or marine chemistry?. J. Mar. Biol. Assoc. UK 77 (1997) 195-210
52. Rainbow P.S. Phylogeny of trace metal accumulation in crustaceans. In: Langston W.J., and Bebianno M.J. (Eds). Metal Metabolism in Aquatic Environments (1998), Chapman and Hall, London 285-319
53. Regoli F., and Orlando E. Accumulation and subcellular distribution of metals (Cu, Fe, Mn, Pb and Zn) in the Mediterranean mussel Mylilus galloprovincialis during a field transplant experiment. Mar. Pollut. Bull. 28 (1994) 592-600
54. Sanchez-Galan S., Linde A.R., and Garcia-Vazquez E. Brown trout and European minnow as target species for genotoxicity tests: differential sensitivity to heavy metals. Ecotoxicol. Environ. Saf. 43 (1999) 301-304
55. Shurgart L.R. Quantitation of chemically induced damage to DNA of aquatic organisms by alkaline unwinding assay. Aquat. Toxicol. 13 (1988) 43-52
56. Shugart L.R. An alkaline unwinding assay for the detection of DNA damage in aquatic organisms. Mar. Environ. Res. 24 (1988) 321-325
57. Silva A.M.M., Novelli E.L.B., Fascineli M.L., and Almeida J.A. Impact of an environmentally realistic intake of water contaminants and superoxide formation on tissues of rats. Environ. Pollut. 105 (1999) 243-249
58. Silvestre F., Duchene C., Trausch G., and Devos P. Tissue-specific cadmium accumulation and metallothionein-like protein levels during acclimation process in the Chinese crab Eriocheir sinensis. Comp. Biochem. Physiol., C 140 (2005) 39-45
59. Siraj Basha P., and Usha Rani A. Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicol. Environ. Saf. 56 (2003) 218-221
60. Siu W.H.L., Hung C.L.H., Wong H.L., Richardson B.J., and Lam P.K.S. Exposure and time dependent DNA strand breakage in hepatopancreas of green-lipped mussels (Perna viridis) exposed to Aroclor 1254, and mixtures of B[a]P and Aroclor 1254. Mar. Pollut. Bull. 46 (2003) 1285-1293
61. Splittgerber A.G., and Tapple A.L. Inhibition of glutathione peroxidase by cadmium and other metals. Arch. Biochem. Biophys. 197 (1979) 534-542
62. Stohs S.T., and Bagchi D. Oxidative mechanisms in the toxicity of metals. Free Radic. Biol. Med. 18 (1995) 321-326
63. Venugopal N.B.P.K., Ramesh T.V.D.D., Reddy D.S., and Reddy S.L.N. Effect of cadmium on antioxidant enzyme activities and lipid peroxidation in a freshwater field crab, Barytelphusa guerini. Bull. Environ. Contam. Toxicol. 59 (1997) 132-138
64. Viarengo A. Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialis Lam. Comp. Biochem. Physiol., C 97 (1990) 37-42
65. Webb M. Role of metallothionein in cadmium metabolism. In: Foulkes E.C. (Ed). Handbook of Experimental Pharmacology. Cadmium vol. 80 (1986), Springer-Verlag, New York 281-337
66. Winston G.W., and Di Giulio R.T. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat. Toxicol. 19 (1991) 137-161
67. Xia Y.M., and Zhu L.Z. Measurement method of glutathione peroxidase activity in blood and tissue. J. Hyg. Res. 16 (1987) 29-33
68. Xu H.Z., Zhou C.G., Ma Y.A., et al. Environmental quality of deposits in offshore zone of China. Environ. Protect. Transportation 21 (2000) 16-18
69. Xu X.D., Lin Z.H., and Li S.Q. The studied of the heavy metal pollution of Jiaozhou Bay. Mar. Sci. 29 (2005) 48-53