cDNA cloning and cadmium-induced expression of metallothionein mRNA in the zebra mussel Dreissena polymorpha

Biochemistry and Cell Biology

Volumn 77, Issue 3, 1999, Pages 237-241

  Engelken, Jens ^a   Hildebrandt, Armin ^a  

^a UNIVERSITY OF BREMEN   (Germany)

Author keywords

Cadmium; Dreissena polymorpha; Induction; Metallothionein; mRNA

Indexed keywords

AMINO ACID; CADMIUM; COMPLEMENTARY DNA; MESSENGER RNA; METALLOTHIONEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; DNA SEQUENCE; GENE EXPRESSION; MOLECULAR CLONING; MUSSEL; NONHUMAN; NUCLEOTIDE SEQUENCE;

DREISSENA; DREISSENA POLYMORPHA; EUKARYOTA; INVERTEBRATA;

EID: 0033379043     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o99-026     Document Type: Article

References (25)

1. Berger, B., Hunziker, P.E., Hauer, C.R., Birchler, N., and Dallinger, R. 1995. Mass spectrometry and amino acid sequencing of two cadmium-binding metallothionein isoforms from the terrestrial gastropod Arianta arbustorum. Biochem. J. 311: 951-957.
2. Bonham, K., Zafarullah, M., and Gedamu, L. 1987. The rainbow trout metallothioneins: Molecular cloning and characterization of two distinct cDNA sequences. DNA 6(6): 519-528.
3. Chomczynski, P., and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
4. Cohen, J. 1994. Flood flexes its mussels. Science (Washington, D.C.), 263: 1226.
5. Dallinger, R., Berger, B., Hunziker, P.E., Birchler, N., Hauer, C.R., and Kägi, J.H.R. 1993. Purification and primary structure of snail metallothionein. Similarity of the N-terminal sequence with histones H4 and H2A. Eur. J. Biochem. 216: 739-746.
6. Dallinger, R., Berger, B., and Kägi, J-H. 1997. Metallothionein in snail Cd and Cu metabolism, Nature (London), 388: 237.
7. Doherty, F.G., Evans, D.W., and Neuhauser, E.F. 1993. An assessment of total and leachable contaminants in zebra mussels (Dreissena polymorpha) from Lake Erie. Ecotoxicol. Environ. Saf. 25: 328-340.
8. Freedman, J.H., Slice, L.W., Dixon, D., Fire, A., and Rubin, C.S. 1993. The novel metallothionein genes of Caenorhabditis elegans. Structural organization and inducible, cell-specific expression, J. Biol. Chem. 268: 2554-2564.
9. Hamer, D.H. 1986. Metallothionein. Ann. Rev. Biochem. 55: 913-951.
10. High, K.A., Barthet, V.J., McLaren, J.W., and Blais, J.S. 1997. Characterization of metallothionein-like proteins from zebra mussels (Dreissena polymorpha). Environ. Toxicol. Chem. 16: 1111-1118.
11. Imagawa, M., Onozawa, T., Okumura, K., Osada, S., Nishimura, T., and Kondo, M. 1990. Characterization of metallothionein cDNAs induced by cadmium in the nematode Caenorhabditis elegans. Biochem. J. 268: 237-240.
12. Kägi, J.H.R., and Schäfer, A. 1988. Biochemistry of Metallothionen. Perspect. Biochem. 27: 8509-8515.
13. Mackay, E.A., Overnell, J., Dunbar, B., Davidson, I., Hunziker, P.E., Kägi, J.H.R., and Fothergill, J.E. 1993. Complete amino acid sequences of five dimeric and four monomeric forms of metallothionein from the edible mussel Mytilus edulis. Eur. J. Biochem. 218: 183-194.
14. Nader, W.F., Edlind, T.D., Huettermann, A., and Sauer, H.W. 1985. Cloning of Physarum actin sequences in an exonuclease-deficient bacterial host. Proc. Natl. Acad. Sci. U.S.A. 82: 2698-2702.
15. Nemer, M., Thornton, R.D., Stuebing, E.W., and Harlow, P. 1991. Structure, spatial, and temporal expression of two sea urchin metallothionein genes, SpMTB1 and SpMTA, J. Biol. Chem. 266: 6586-6593.
16. Pearson, W.R., and Lipman, D.J. 1988. Improved tools for biological sequence comparison, Proc. Natl. Acad. Sci. U.S.A. 85: 2444-2448.
17. Peterson, M.G., Lazdins, I., Danks, D.M., and Mercer, J.F. 1984. Cloning and sequencing of a sheep metallothionein cDNA, Eur. J. Biochem. 143: 507-511.
18. Saint-Jacques, E., Guay, J., Wirtanen, L., Huard, V., Stewart, G., and Seguin, C. 1998. Cloning of a complementary DNA encoding an Ambystoma mexicanum metallothionein, AmMT, and expression of the gene during early development. DNA Cell. Biol. 17: 83-91.
19. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Schlenk, D., and Brouwer, M. 1993. Induction of metallothionein mRNA in the blue crab (Callinectes sapidus) after treatment with cadmium. Comp. Biochem. Physiol. 104C: 317-321.
21. Sures, B., Taraschewski, H., and Rydlo, M. 1997. Intestinal fish parasites as heavy metal bioindicators: A comparison between Acanthocephalus lucii (Palaeacantho-cephala) and the zebra mussel, Dreissena polymorpha. Bull. Environ. Contam. Toxicol. 52: 269-273.
22. 2+. Ecotoxicol. Environ. Saf. 33: 246-252.
23. Unger, M.E., and Roesijadi, G. 1993. Sensitive assay for molluscan metallothionein induction based on ribonuclease protection and molecular titration of metallothionein and actin mRNAs. Mol. Mar. Biol. Biotechnol. 2: 319-324.
24. Unger, M.E., Chen, T.T., Murphy, C.M., Vestling, M.M., Fenselau, C., and Roesijadi, G. 1991. Primary structure of molluscan metallothioneins deduced from PCR-amplified cDNA and mass spectrometry of purified proteins. Biochim. Biophys. Acta, 1074: 371-377.
25. Waalkes, M.P., and Goering, P.L. 1990. Metallothionein and other cadmium-binding proteins: Recent developments. Chem. Res. Toxicol. 3: 281-288.