Genotoxic effects of cadmium chloride and azadirachtin treated singly and in combination in fish

Ecotoxicology and Environmental Safety

Volumn 58, Issue 2, 2004, Pages 194-201

  Chandra, P ^a   Khuda Bukhsh, A R ^a  

^a UNIVERSITY OF KALYANI   (India)

Author keywords

Azadirachtin; Azadiracta indica; Cadmium chloride; Chromosome aberrations; Genotoxicity; Nuclear anomaly; SDS PAGE; Sperm head anomaly

Indexed keywords

AZADIRACHTIN; CADMIUM CHLORIDE;

CADMIUM; GENOTOXICITY; NONTARGET ORGANISM; PERCIFORM;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL NUCLEUS; CELL STRUCTURE; CHROMOSOME ABERRATION; CONTROLLED STUDY; ERYTHROCYTE; FISH; GENOTOXICITY; NONHUMAN; PROTEIN CONTENT; SPERM;

ANIMALS; AZADIRACHTA; CADMIUM CHLORIDE; CELL NUCLEUS; CHROMOSOME ABERRATIONS; DRUG COMBINATIONS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTES; INJECTIONS, INTRAMUSCULAR; INSECTICIDES; KARYOTYPING; LIMONINS; MALE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MUTAGENICITY TESTS; PLANT EXTRACTS; PROTEINS; SPERM HEAD; TILAPIA; TISSUE DISTRIBUTION; WATER POLLUTANTS, CHEMICAL;

AZADIRACHTA INDICA; OREOCHROMIS; OREOCHROMIS MOSSAMBICUS;

EID: 2442473888     PISSN: 01476513     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ecoenv.2004.01.010     Document Type: Article

References (42)

1. Awasthy K.S., Chaurasia O.P., Sinha S.P. Genotoxic effects of crude extract of neem (Azadirachta indica) in bone marrow cells of mice. Cytologia. 60:1995;189-193
2. Bruce W., Heddle J. The mutagenic activity of 61 agents as determined by the micronucleus, salmonella and sperm abnormality assay. Can. J. Genet. Cytol. 21:1979;319-334
3. Carrasco K.R., Tilbury K.L., Myers M.S. Assessment of the piscine micronucleus test as in situ biological indicator of chemical contaminant effects. Can. J. Fish. Aquat. Sci. 47:1990;2123-2136
4. Chandra, P., Khuda-Bukhsh, A.R., 2001. An assay of genotoxicity produced by cadmium chloride in fish (Oreochromis mossambicus) and efficacy of vitamin C in its alterations. In: Manna, G.K., Roy, S.C. (Eds.), Perspectives in Cytology and Genetics. AICCG Publ., Kalyani, India, 10, pp. 583-590.
5. Dang Z.C., Berntssen M.H., Lundebye A.K., Flik G., Wendelaar Bonga S.E, Lock R.A. Metallothionein and cortisol receptor expression in gills of Atlantic salmon, Salmo salar, exposed to dietary cadmium. Aquat. Toxicol. 53:2001;91-101
6. Deknudt G., Gerber G. Chromosomal aberrations in bone marrow cells of mice given a normal or a calcium deficient diet supplemented with various heavy metals. Mutat. Res. 68:1979;163-168
7. Felten T. A preliminary report of cadmium-induced chromosomal changes in somatic and germinal tissues of C57BL/GJ male mice. Genetica. 88:1978;26-27
8. Guha, B., Khuda-Bukhsh, A.R., 2001. Cytogenetic effects of ethyl methane sulphonate (EMS) and neem extract (Azadirachtin) on the fish Oreochromis mossambicus as a model. In: Manna, G.K., Roy, S.C. (Eds.), Perspectives in Cytology and Genetics, AICCG Publ., Kalyani, India, 10, pp. 551-559.
9. Guha B., Khuda-Bukhsh A.R. Assessment of EMS-induced genotoxicity in the Indian climbing perch, Anabas testudineus. cytogenetical vis-à-vis protein endpoints Indian J. Exp. Biol. 40:2002;1285-1294
10. Guha B., Khuda-Bukhsh A.R. Efficacy of vitamin-C (L-ascorbic acid) in reducing genotoxicity in fish (Oreochromis mossambicus) induced by ethyl methane sulphonate. Chemosphere. 47:2002;49-56
11. Guha B., Khuda-Bukhsh A.R. Ameliorating effects of β-carotene on ethyl methanesulphonate induced genotoxicity in a fish, Oreochromis Mossambicus. Mutat. Res. 542:2003;1-13
12. Hartwig A. Role of DNA repair inhibition in lead- and cadmium-induced genotoxicity. a review Environ. Health Perspect. 102:1994;45-50
13. IARC, 1993. Monograph on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. No. 58, IARC, Lyon, pp. 119-237.
14. Iliopoulou-Georgudaki J., Kotsanis N. Toxic effects of cadmium and mercury in rainbow trout (Oncorhynchus mykiss). A short-term bioassay. Bull. Environ. Contam. Toxicol. 66:2001;77-85
15. Jagetia J.C., Adiga S.K. Cadmium chloride induces dose-dependent increase in the frequency of micronuclei in mouse bone marrow. Mutat. Res. 306:1994;85-90
16. Kalinina L., Ploukhina G. Mutagenic effect of heavy metal on salmonella inactivation systems in vivo and in vitro. Mutat. Res. 46:1977;223-224
17. Kar R.N., Das R.K. Dominant lethality and spermatocytic chromosome aberrations in mice treated with chlordiazepoxide. Caryologia. 40:1987;267-273
18. Khuda-Bukhsh A.R. Chromosomes in three species if fishes, Aplocheilus panchax (Cyprinodontidae), Lates calcarifer (Percidae) and Gadusia chapra (Clupeidae). Caryologia. 32:1979;161-169
19. Klopman G., Conttreras R., Reosenkranz H.S., Waters M.D. Structure-genotoxic activity relationships of pesticides. comparison of the results from several short-term assays Mutat. Res. 147:1985;343-356
20. Kotsanis N., Iliopoulou-Georgoudaki J. Toxic effects of cadmium at early stages of rainbow trout (Oncorhynchus mykiss). Toxicol. Environ. Chem. 62:1997;197-205
21. Laemmli U.K. Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature. 227:1970;680-685
22. Leonard A. Carcinogenic and mutagenic effects of metals (As, Cd, Cr, Hg, Ni), present state of knowledge and needs for further studies. Di Ferrante E. Trace Metals Exposure and Health Effects. 1979;199-216 Pergamon Press, London
23. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193:1951;265-275
24. Mance G. Toxicity of metals to freshwater fish. Mance G. Pollution Threat of Heavy Metals in Aquatic Environments, Pollution Monitoring Series. 1990;22-126 Elsevier Applied Science, London, New York
25. Manna G.K. Progress in fish cytogenetics. Nucleus. 27:1984;203-231
26. Manna G.K., Biswas S. Induction of abnormal sperm head in tilapia fish as means of testing genotoxicity potentiality of the bacterium, Pseudomonas aoruginosa. La kromosomo II. 51-52:1988;1659-1664
27. Marazzini A.C., Betti C., Bernachi F., Barrai I., Barale R. Micronucleus test and metaphase analyses in mice exposed to suspected spindle poison. Mutagenesis. 9:1994;505-515
28. Martins C., Wasko A.P., Oliveira C., Wright J.M. Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the tilapiine cichlid fish, Oreochromis niloticus. Caryologia. 133:2000;39-46
29. Martins C., Wasko A.P., Oliveira C., Porto-Foresti F., Parise-Masltempi P.P., Wright J M., Foresti F. Dynamics of 5 S rDNA in the tilapia (Oreochromis niloticus) genome. repeat units, inverted sequences, pseudogenes and chromosome loci Cytogenet. Genome Res. 98:2002;78-85
30. Mavournin K.H., Blakey D.H., Cimino M.C., Salamone M.F., Heedle J.A. The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the US Environmental Protection Agency genetox program. Mutat. Res. 239:1990;29-80
31. Metcalfe C.D. Test for predicting carcinogenicity in fish, CRC Critical Reviews. Aquat. Sci. 1:1989;111-112
32. Rosenkranz H.S., Klopman G. An examination of the potential genotoxic carcinogenicity of a biopesticide derived from the neem tree. Environ. Mol. Mutagen. 26:1995;255-260
33. Schmid W. The micronucleus test for cytogenetic analysis. Hollaender A. Chemical Mutagens Principles and Methods of Detection. Vol. 4:1976;31-53 Plenum Press, New York
34. Schmidt, G.H., Pesel, E., 1987. Studies on sterilizing effect of neem extracts in ants. In: Proceedings of the Third International Neem Conference, Nairibi, Kenya, July 10, 1986, pp. 361-372.
35. Sharma V.P., Dhiman R.C. Neem oil as sand fly (Diptera: Psychodidae) repellant. J. Am. Mosq. Control Assoc. 9:1993;364-366
36. Shaw C.R. How many genes evolved? Biochem. Genet. 4:1970;275-283
37. Sies H. Strategies of antioxidant defense. Eur. J. Biochem. 215:1993;213-219
38. Tsuzuki K., Sugiyama M., Haramaki N. DNA-single strand break and cytotoxicity induced by chromate (VI), cadmium (II) and mercury (II) in hydrogen peroxide-resistant cell lines. Environ. Health Perspect. 102:1994;341-342
39. Van Der Nat M.G., Van Der Sluis K.T.D., Labadie R.P. Ethnopharmocognostical survey of A. indica Juss (Meliaceae). J. Ethnopharm. 35:1991;1-24
40. Voet D., Voet J.G. Biochemistry. 2nd Edition:1995;Wiley, New York
41. Warbric E.V., Barker G.C., Rees H.H., Howells R.E. The effect of invertebrate hormones and potential hormone inhibitors on the third larval moult of the filarial nematode, Dicrofilaria immitis in vitro. Parasitology. 107:1993;459-463
42. Weigant F.A.C., Van Rijn J., Van Wijk R. Enhancement of the stress response by minute amounts of cadmium in sensitized Reuber H35 hepatoma cells. Toxicology. 116:1997;27-37