Method for preparing single-stranded DNA templates for Pyrosequencing using vector ligation and universal biotinylated primers

Analytical Biochemistry

Volumn 356, Issue 2, 2006, Pages 194-201

  Groth, Marco ^a   Huse, Klaus ^a   Reichwald, Kathrin ^a   Taudien, Stefan ^a   Hampe, Jochen ^b   Rosenstiel, Philip ^b   Birkenmeier, Gerd ^c   Schreiber, Stefan ^b   Platzer, Matthias ^a  

^a FRITZ LIPMANN INSTITUTE   (Germany)

^b UNIVERSITY OF KIEL   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

DNA labeling; DNA sequencing; Pyrosequencing; Single nucleotide polymorphism; Template preparation; Topoisomerase; Universal biotinylated primer

Indexed keywords

DNA; DNA SEQUENCES; GENE ENCODING; NUCLEOTIDES; PROTEINS;

DNA SEQUENCING; PYROSEQUENCING; SINGLE NUCLEOTIDE POLYMORPHISMS; TEMPLATE PREPARATION; TOPOISOMERASES; UNIVERSAL BIOTINYLATED PRIMER;

POLYMERASE CHAIN REACTION;

ALPHA 2 MACROGLOBULIN; CASPASE RECRUITMENT DOMAIN PROTEIN 15; PLASMID VECTOR; PRIMER DNA; SINGLE STRANDED DNA;

ARTICLE; BIOTINYLATION; DNA DETERMINATION; DNA ISOLATION; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; GENE DUPLICATION; GENE MAPPING; GENETIC ANALYSIS; GENETIC POLYMORPHISM; GENETIC VARIABILITY; GENOTYPE; HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM;

EID: 33747882195     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.04.043     Document Type: Article

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