Method for one-step preparation of double-stranded DNA template applicable for use with Pyrosequencing™ technology

Journal of Biochemical and Biophysical Methods

Volumn 52, Issue 2, 2002, Pages 71-82

  Nordström, Tommy ^a   Alderborn, Anders ^b   Nyrén, Pål ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b Pyrosequencing AB   (Sweden)

Author keywords

Blocking oligonucleotides; DNA sequencing; Double stranded DNA; Pyrosequencing; Single nucleotide polymorphism; Template preparation

Indexed keywords

DIMER; DNA BINDING PROTEIN; DOUBLE STRANDED DNA; EXODEOXYRIBONUCLEASE I; EXONUCLEASE; OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; DNA SEQUENCE; DNA TEMPLATE; GENOTYPE; NUCLEOTIDE METABOLISM; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RENIN ANGIOTENSIN ALDOSTERONE SYSTEM; SEQUENCE ANALYSIS; TECHNOLOGY;

BASE SEQUENCE; BIOTECHNOLOGY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIMERIZATION; DNA; DNA PRIMERS; DNA, SINGLE-STRANDED; GENOTYPE; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; RENIN-ANGIOTENSIN SYSTEM; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

EID: 0037205983     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(02)00034-9     Document Type: Article

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