Detection and quantification of Legionella pneumophila in water samples using competitive PCR

Canadian Journal of Microbiology

Volumn 52, Issue 6, 2006, Pages 584-590

  Declerck, Priscilla ^a   Behets, Jonas ^a   Lammertyn, Elke ^b   Lebeau, Ilya ^b   Anné, Jozef ^b   Ollevier, Frans ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

Competitive PCR; Cooling tower water; Cost effective; Legionella pneumophila; Sensitive detection; Tap water

Indexed keywords

COST EFFECTIVENESS; DISEASES; ENVIRONMENTAL IMPACT; MICROBIOLOGY; PATHOLOGY;

COMPETITIVE PCR; COOLING TOWER WATER; LEGIONELLA PNEUMOPHILA; SENSITIVE DETECTION; TAP WATER;

BACTERIA;

TAP WATER;

AQUATIC ECOSYSTEM; BACTERIAL DISEASE; BACTERIUM; DETECTION METHOD; DRINKING WATER; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

ACCURACY; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; COOLING TOWER; COST EFFECTIVENESS ANALYSIS; LEGIONELLA PNEUMOPHILA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WATER SAMPLING;

COLONY COUNT, MICROBIAL; GENOME, BACTERIAL; HUMANS; LEGIONELLA PNEUMOPHILA; LEGIONNAIRES' DISEASE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; WATER MICROBIOLOGY;

LEGIONELLA PNEUMOPHILA;

EID: 33747505380     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W05-156     Document Type: Article

References (27)

1. Aurell, H., Catala, P., Farge, P., Wallet, F., Le Brun, M., Helbig, J.H., Jarraud, S., and Lebaron, P. 2004. Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry. Appl. Environ. Microbiol. 70: 1651-1657.
2. Ballard, A.L., Fry, N.K., Chan, L., Surman, S.B., Lee, J.V., Harrison, T.G., and Towner, K.J. 2000. Detection of Legionella pneumophila using a real-time PCR hybridization assay. J. Clin. Microbiol. 38: 4215-4218.
3. Best, M., Yu, V.L., Stout, J., Goetz, A., Muder, R.R., and Taylor, F. 1983. Legionellaceae in the hospital water-supply. Epidemiological link with disease and evaluation of a method for control of nosocomial Legionnaires' disease and Pittsburgh pneumonia. Lancet, ii: 307-310.
4. Bjerrum, L., Kjaer, T., and Ramsing, N.B. 2002. Enumerating ammonia-oxidizing bacteria in environmental samples using competitive PCR. J. Microbiol. Meth. 51: 227-239.
5. Declerck, P., Verelst, L., Duvivier, L., Van Damme, A., and Ollevier, F. 2003. PCR as a test for the presence or absence of Legionella in (cooling) water. Water Sci. Technol. 47: 103-107.
6. Declerck, P., Behets, J., Delaedt, Y., Margineanu, A., Lammertyn, E., and Ollevier, F. 2005. Impact of non-Legionella bacteria on the uptake and intracellular replication of Legionella pneumophila in Acanthamoeba castellanii and Naegleria lovaniensis. Microbial Ecol. 50(4): 536-549.
7. Doungudomdacha, S., Rawlinson, A., and Douglas, C.W.I. 2000. Enumeration of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in subgingival plaque samples by a quantitative- competitive PCR method. J. Med. Microbiol. 49: 861-874.
8. Hayden, R.T., Uhl, J.R., and Qian, X. 2001. Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of lightcycler PCR, in situ hybridization, direct fluorescence antigen detection and culture. J. Clin. Microbiol. 39: 2618-2626.
9. Ikonomopoulos, J., Gazouli, M., Pavlik, I., Bartos, M., Zacharatos, P., Xylouri, E., Papalambros, E., and Gorgoulis, V. 2004. Comparative evaluation of PCR assays for the robust molecular detection of Mycobacterium avium subsp. paratuberculosis. J. Microbiol. Meth. 56: 315-321.
10. International Organization for Standardization. 1998. Water quality part 4: microbiological methods. Section 4.12: detection and enumeration of Legionella. ISO, Geneva, Switzerland.
11. Knut, R., Skulberg, O.M., Larsen, F., and Jakobsen, K.S. 1998. Quantification of toxic cyanobacteria in water by use of competitive PCR followed by sequence-specific labeling of oligonucleotide probes. Appl. Environ. Microbiol. 64: 2639-2643.
12. Levi, K., Smedley, J., and Towner, K.J. 2003. Evaluation of a real-time PCR hybridization assay for rapid detection of Legionella pneumophila in hospital and environmental water samples. Clin. Microbiol. Infect. 9: 754-758.
13. Martson, B.J., Lipman, H.B., and Breiman, R.F. 1994. Surveillance for Legionnaires' disease. Risk factors for morbidity and mortality. Arch. Intern. Med. 154: 2417-2422.
14. Mauchline, T.H., Kerry, B.R., and Hirsch, P.R. 2002. Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating. Appl. Environ. Microbiol. 68: 1846-1853.
15. Meigh, R.E., Makin, T., Scott, M.H., and Hart, C.A. 1989. Legionella pneumophila serogroup 12 pneumonia in a renal transplant recipient: case report and environmental observations. J. Hosp. Infect. 13: 315-319.
16. Miyamoto, H., Yamamoto, H., Arima, K., Fujii, J., Maruta, K., Izu, K., Shiomori, T., and Yoshida, S.I. 1997. Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water. Appl. Environ. Microbiol. 63: 2489-2494.
17. Mumy, K.L., and Findlay, R.H. 2004. Convenient determination of DNA extraction efficiency using an external DNA recovery standard and quantitative-competitive PCR. J. Microbiol. Meth. 57: 259-268.
18. Orlando, C., Pinzani, P., and Pazzagli, M. 1998. Developments in quantitative PCR. Clin. Chem. Lab. Med. 36: 255-269.
19. Reilly, K., and Attwood, G.T. 1998. Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR. Appl. Environ. Microbiol. 64: 907-913.
20. Riboldi-Tunnicliffe, A., Konig, B., Jessen, S., Weiss, M.S., Rahfeld, J., Hacker, J., Fischer, G., and Hilgenfeld, R. 2001. Crystal structure of Mip, a prolylisomerase from Legionella pneumophila. Nat. Struct. Biol. 8: 779-783.
21. Steinert, M., Emödy, L., Amann, R., and Hacker, J. 1997. Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii. Appl. Environ. Microbiol. 63: 2047-2053.
22. Templeton, K.E., Scheltinga, S.A., Sillekens, P., Crielaard, J.W., van Dam, A.P., Goossens, H., and Claas, E.C.J. 2003. Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species. J. Clin. Microbiol. 41: 4016-4021.
23. Toze, S. 1999. PCR and the detection of microbial pathogens in water and wastewater. Water Res. 33: 3545-3556.
24. Tsai, Y.L., and Rochelle, P.A. 2001. Extraction of nucleic acids from environmental samples. In Environmental molecular microbiology: protocols and applications. Edited by P.A. Rochelle. Horizon Scientific Press, Wymondham, UK. pp. 15-30.
25. Villari, P., Motti, E., Farullo, C., and Torre, I. 1998. Comparison of conventional culture and PCR methods for the detection of Legionella pneumophila in water. Lett. Appl. Microbiol. 27: 106-110.
26. Wellinghausen, N., Frost, C., and Marre, R. 2001. Detection of legionellae in hospital water samples by quantitative real-time lightcycler PCR. Appl. Environ. Microbiol. 67: 3985-3993.
27. Yu, V.L., Plouffe, J.F., Pastoris, M.C., Stout, J.E., Schousboe, M., Widmer, A., Summersgill, J., File, T., Heath, C.M., Paterson, D.L., and Chereshsky, A. 2002. Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. J. Infect. Dis. 186: 127-128.