A decerebrate, artificially-perfused in situ preparation of rat: Utility for the study of autonomic and nociceptive processing

Journal of Neuroscience Methods

Volumn 155, Issue 2, 2006, Pages 260-271

  Pickering, Anthony E ^a,b   Paton, Julian F R ^b  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Artificial perfusion; Baroreflex; In situ preparation; Nociception; Rat; Spinal cord; Sympathetic

Indexed keywords

ADRENAL GLAND; ANIMAL EXPERIMENT; ARTICLE; AUTONOMIC NERVOUS SYSTEM; BLADDER; BRAIN STEM; BREATHING PATTERN; CELL COMMUNICATION; CHEMORECEPTOR REFLEX; COLLOID; CONTROLLED STUDY; DECEREBRATION; EXTRACELLULAR MATRIX; HEART LEFT VENTRICLE; HINDLIMB; INTRACELLULAR RECORDING; NOCICEPTION; NONHUMAN; PERFUSION; PRESSORECEPTOR REFLEX; PRIORITY JOURNAL; RAT; SPINAL CORD; SPLANCHNIC BLOOD FLOW; SYMPATHETIC FUNCTION; SYMPATHETIC TONE; TAIL;

ACTION POTENTIALS; ANIMALS; AUTONOMIC NERVOUS SYSTEM; DECEREBRATE STATE; HEART RATE; MODELS, ANIMAL; PAIN; PAIN MEASUREMENT; PERFUSION; POSTERIOR HORN CELLS; RATS; RATS, WISTAR; SPINAL CORD; VISCERAL AFFERENTS;

EID: 33747403865     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2006.01.011     Document Type: Article

References (31)

1. Adrian E.D., Bronk D.W., and Phillips G. Discharges in mammalian sympathetic nerves. J Physiol 74 (1932) 115-133
2. Bennett G.J., and Xie Y.K. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain 33 (1988) 87-107
3. Bourque C.W., and Renaud L.P. A perfused in vitro preparation of hypothalamus for electrophysiological studies on neurosecretory neurons. J Neurosci Methods 7 (1983) 203-214
4. Cervero F., and Laird J.M. Understanding the signaling and transmission of visceral nociceptive events. J Neurobiol 61 (2004) 45-54
5. Chizh B.A., Headley P.M., and Paton J.F. An arterially-perfused trunk-hindquarters preparation of adult mouse in vitro. J Neurosci Methods 76 (1997) 177-182
6. Dingledine R. Brain slices (1989), Plenum Press, New York
7. Dubuisson D., and Dennis S.G. The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain 4 (1977) 161-174
8. Dutschmann M., and Paton J.F. Dynamic changes in glottal resistance during exposure to severe hypoxia in neonatal rats in situ. Pediatr Res 58 (2005) 193-198
9. Dutschmann M., and Paton J.F. Whole cell recordings from respiratory neurones in an arterially perfused in situ neonatal rat preparation. Exp Physiol 88 (2003) 725-732
10. Edwards F.A., Konnerth A., Sakmann B., and Takahashi T. A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system. Pflugers Arch 414 (1989) 600-612
11. Fulton B.P., and Walton K. Electrophysiological properties of neonatal rat motoneurones studied in vitro. J Physiol 370 (1986) 651-678
12. Furue H., Narikawa K., Kumamoto E., and Yoshimura M. Responsiveness of rat substantia gelatinosa neurones to mechanical but not thermal stimuli revealed by in vivo patch-clamp recording. J Physiol 521 Pt 2 (1999) 529-535
13. Graham B.A., Brichta A.M., and Callister R.J. An in vivo mouse spinal cord preparation for patch-clamp analysis of nociceptive processing. J Neurosci Methods 136 (2004) 221-228
14. Habler H.J., Janig W., and Michaelis M. Respiratory modulation in the activity of sympathetic neurones. Prog Neurobiol 43 (1994) 567-606
15. Hayashi F., Jiang C., and Lipski J. Intracellular recording from respiratory neurones in the perfused 'in situ' rat brain. J Neurosci Methods 36 (1991) 63-70
16. Kim S.H., and Chung J.M. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain 50 (1992) 355-363
17. Lewis T. Pain (1942), MacMillan, New York
18. Light A.R., and Willcockson H.H. Spinal laminae I-II neurons in rat recorded in vivo in whole cell, tight seal configuration: properties and opioid responses. J Neurophysiol 82 (1999) 3316-3326
19. Llinas R., and Muhlethaler M. An electrophysiological study of the in vitro, perfused brain stem-cerebellum of adult guinea-pig. J Physiol 404 (1988) 215-240
20. Llinas R.R. The intrinsic electrophysiological properties of mammalian neurons: insights into central nervous system function. Science 242 (1988) 1654-1664
21. Morin-Surun M.P., and Denavit-Saubie M. Rhythmic discharges in the perfused isolated brainstem preparation of adult guinea pig. Neurosci Lett 101 (1989) 57-61
22. Muhlethaler M., de Curtis M., Walton K., and Llinas R. The isolated and perfused brain of the guinea-pig in vitro. Eur J Neurosci 5 (1993) 915-926
23. Paton J.F. A working heart-brainstem preparation of the mouse. J Neurosci Methods 65 (1996) 63-68
24. Paton J.F., Li Y.W., and Schwaber J.S. Response properties of baroreceptive NTS neurons. Ann N Y Acad Sci 940 (2001) 157-168
25. Paton J.F., and St-John W.M. Long-term intracellular recordings of respiratory neuronal activities in situ during eupnea, gasping and blockade of synaptic transmission. J Neurosci Methods 147 (2005) 138-145
26. Pickering A.E., Waki H., Headley P.M., and Paton J.F. Investigation of systemic bupivacaine toxicity using the in situ perfused working heart-brainstem preparation of the rat. Anesthesiology 97 (2002) 1550-1556
27. Potts J.T., Paton J.F., Mitchell J.H., Garry M.G., Kline G., Anguelov P.T., and Lee S.M. Contraction-sensitive skeletal muscle afferents inhibit arterial baroreceptor signalling in the nucleus of the solitary tract: role of intrinsic GABA interneurons. Neuroscience 119 (2003) 201-214
28. Richerson G.B., and Getting P.A. Maintenance of complex neural function during perfusion of the mammalian brain. Brain Res 409 (1987) 128-132
29. Simms A.E., Antunes V.R., Murphy D., Paton J., and Pickering A.E. Use of an in situ artificially perfused whole rat preparation to explore central control of the sympathetic nervous system. J Physiol (2005) 567P:D2
30. St-John W.M., and Paton J.F. Characterizations of eupnea, apneusis and gasping in a perfused rat preparation. Respir Physiol 123 (2000) 201-213
31. Wong L.F., Polson J.W., Murphy D., Paton J.F., and Kasparov S. Genetic and pharmacological dissection of pathways involved in the angiotensin II-mediated depression of baroreflex function. FASEB J 16 (2002) 1595-1601