Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis

Biochemical Engineering Journal

Volumn 31, Issue 1, 2006, Pages 25-30

  Farkade, Vivek D ^a   Harrison, Susan T L ^b   Pandit, Aniruddha B ^a  

^a UNIVERSITY OF MUMBAI   (India)

^b UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Cavitation; Cell disruption; Enzyme release; pH treatment

Indexed keywords

CAVITATION; ENERGY EFFICIENCY; ENZYMES; EXTRACTION; PH EFFECTS; ULTRASONIC APPLICATIONS;

CELL DISRUPTION; DISRUPTION PROCESS; ENZYME RELEASE; PH TREATMENT;

CELL CULTURE;

BETA GALACTOSIDASE; FUNGAL ENZYME;

AQUEOUS SOLUTION; ARTICLE; CELL DISRUPTION; ENERGY; ENZYME RELEASE; EXTRACTION; KLUYVEROMYCES; NONHUMAN; PH; PRIORITY JOURNAL; ULTRASOUND;

EID: 33747369621     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2006.05.015     Document Type: Article

References (32)

1. Edebo L. Disintegration of cells. Ferment. Adv. (1969) 249-270
2. Chisti Y., and Moo-Young M. Disruption of microbial cells for intracellular products. Enzyme Microbial Technol. 8 (1986) 194-204
3. Save S.S., Pandit A.B., and Joshi J.B. Microbial cell disruption: role of cavitation. Chem. Eng. J. 55 (1994) B67-B72
4. Balasundaram B., and Pandit A.B. Selective release of invertase by hydrodynamic cavitation. Biochem. Eng. J. 8 (2001) 251-256
5. Schutte H., Kula M.R., Kroner K., and Hustedt H. Experiences with a 20-litre industrial bead mill for the disruption of microorganisms. Enzyme Microbial Technol. 5 (1983) 143-148
6. Kula M.R., and Schutte H. Purification of proteins and the disruption of microbial cells. Biotechnol. Prog. 3 1 (1987) 31-42
7. Sauer T., Robinson C.W., and Glick B.R. Disruption of native and recombinant E. coli in a high pressure homogenizer. Biotechnol. Bioeng. 33 (1989) 1330-1342
8. Balasundaram B., and Pandit A.B. Significance of location of enzymes on their release during microbial cell disruption. Biotechnol. Bioeng. 75 (2001) 607-614
9. Harrison S.T.L. Bacterial cell disruption: a key unit operation in the recovery of intracellular products. Biotechnol. Adv. 9 (1991) 217-240
10. Doulah M.S. Mechanism of disintegration of biological cells in ultrasonic cavitation. Biotechnol. Bioeng. 19 (1975) 649-660
11. Feliu J.X., and Villaverde A. An Optimized ultrasonication protocol for bacterial cells disruption and recovery of β-galactosidase fusion proteins. Biotechnol. Tech. 8 7 (1994) 509-514
12. Feliu J.X., Cubarshi R., and Villaverde A. Optimized release of recombinant proteins by ultrasonication of E. coli cells. Biotechnol. Bioeng. 58 (1998) 536-540
13. Farkade V.D., Harrison S., and Pandit A.B. Heat induced translocation of proteins and enzymes within the cells: an effective way to optimize the microbial cell disruption process. Biochem. Eng. J. 23 (2005) 247-257
14. Umakoshi U., Kuboi R., and Fukata Y. Utilization of cell response under heat, chemical and combined stresses for the selective recovery of cytoplasmic β-galactosidase from the E. coli cells. Biotechnol. Prog. 14 (1998) 909-912
15. Ariga O., Yoshinaga T., and Sano Y. Efficient release of thermostable β-galactosidase by glycine and thermal treatment of E. coli cells. J Ferment. Bioeng. 79 1 (1995) 76-78
16. Baldwin C.V., and Robinson C.W. Enhanced disruption of Candida utilis using enzymatic pretreatment and high pressure homogenization. Biotechnol. Bioeng. 43 (1994) 46-56
17. Vogels G., and Kula M.R. Combination of enzymatic and/or thermal pretreatment with the mechanical cell disintegration. Chem. Eng. Sci. 47 1 (1992) 123-131
18. Bailey S.M., Blum P.H., and Meagher M.M. Improved homogenization of recombinant E.coli following pre-treatment with Guanidine hydrochloride. Biotechnol. Prog. 11 (1995) 533-539
19. Harrison S.T.L., Dennis J.S., and Chase H.A. Combined chemical and mechanical processes for the disruption of bacteria. Bioseparation 2 (1991) 95-105
20. Fonseca L.P., and Cabral J.M.S. Penicillin acylase release from E. coli cells by mechanical cell disruption and permeabilisation. J. Chem. Technol. Biotechnol. 77 (2002) 159-167
21. Haung R.B., Andrew B., and Asenjo J.A. Differential product release of proteins from yeast: a new technique for selective product recovery from microbial cells. Biotechnol. Bioeng. 38 (1991) 977-985
22. French C., Moore E.K., and Ward J.M. Development of simple method for the recovery of recombinant proteins from the E. coli periplasm. Enzyme Microbial Technol. 19 (1996) 332-338
23. Hettwer D., and Wang H. Protein release from E. coli cells permeabilised with guanidine HCl and Triton X100. Biotechnol. Bioeng. 33 (1989) 886-895
24. Flores M.V., Voget C.E., and Ertola R.J.J. Permeabilization of yeast cells (Kluveromyces lactis) with the organic solvent. Enzyme Microbial Technol. 16 (1994) 340-346
25. Dickson R.C., and Markin J.S. Physiological studies of β-galactosidase induction in Kluveromyces lactis. J. Bacteriol. 142 3 (1980) 777-785
26. Lowry O.H., Rosenborough N.J., Farr A.L., and Randall R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193 (1951) 65-75
27. H.E. Wade, Improvements in or relating to extraction processes, Patent No. 40344168 (London) (1968).
28. Hedenskog G., Mogren H., and Enebo L. A method for obtaining protein concentrates from microorganisms. Biotechnol. Bioeng. 12 (1970) 947-959
29. Craig S., Schmeissner U., Wingfield P., and Pain R.H. Conformation, stability and folding of interleukin 1β. Biochemistry 26 (1987) 3570-3576
30. Kuboi R., Umakoshi H., Takagi N., and Komasawa I. Optimal disruption methods for the selective recovery of β-galactosidase from E. coli. J. Ferment. Bioeng. 79 (1995) 335-341
31. Follows M., Hetherington P.J., and Dunnill P. Release of enzymes from Bakers yeast by disruption in an industrial homogenizer. Biotechnol. Bioeng. 8 (1971) 549-560
32. Torner J., and Asenjo J.A. Kinetics of enzyme release from breadmaking yeast cells in a bead mill. Biotechnol. Tech. 2 (1991) 35-40