Optimized release of recombinant proteins by ultrasonication of E. coli cells

Biotechnology and Bioengineering

Volumn 58, Issue 5, 1998, Pages 536-540

  Feliu, J X ^a   Cubarsi, R ^b   Villaverde, A ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITAT POLITÈCNICA DE CATALUNYA   (Spain)

Author keywords

galactosidase; Cell disruption; E. coli; Fusion proteins; Ultrasonication

Indexed keywords

BACTERIA; ENZYME KINETICS; GROWTH KINETICS; IONIC STRENGTH; PROTEINS;

FUSION PROTEINS; GALACTOSIDASE PROTEIN; RECOMBINANT PROTEINS; ULTRASONICATION;

CELL CULTURE;

HYBRID PROTEIN; RECOMBINANT PROTEIN;

ACOUSTICS; ARTICLE; CYTOLYSIS; ESCHERICHIA COLI; GENETIC RECOMBINATION; NONHUMAN; ULTRASOUND; ULTRASOUND THERAPY;

BETA-GALACTOSIDASE; CLONING, MOLECULAR; ESCHERICHIA COLI; ISOPROPYL THIOGALACTOSIDE; KINETICS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; ULTRASONICS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032486514     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980605)58:5<536::AID-BIT10>3.0.CO;2-9     Document Type: Article

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