Utilization of cell response under heat, chemical, and combined stresses for selective recovery of cytoplasmic β-galactosidase from Escherichia coli cells

Biotechnology Progress

Volumn 14, Issue 6, 1998, Pages 909-912

  Umakoshi, Hiroshi ^a   Fukuta, Yuka ^a   Kuboi, Ryoichi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL METABOLISM; BETA GALACTOSIDASE; BIOSEPARATION; CELL RESPONSE; CYTOPLASM; EDETIC ACID; HEAT TOLERANCE; SECONDARY METABOLITE; STRESS TOLERANCE;

ESCHERICHIA COLI; SEPARATION; STRESS ANALYSIS; THERMAL STRESS;

BIOSEPARATION; CHEMICAL STRESS; CYTOPLASMIC GALACTOSIDASE; SELECTIVE RECOVERY;

ENZYMES;

BETA-GALACTOSIDASE; BIOTECHNOLOGY; CYTOPLASM; EDETIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HEAT; KINETICS; OCTOXYNOL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032210819     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp980091s     Document Type: Article

References (20)

1. Michaelis, S.; Hunt, J. F.; Beckwith, J. Effects of signal sequence mutations on the kinetics of the alkaline phosphatase export to the periplasm in Escherichia coli. J. Bacteriol. 1986, 167, 160-168.
2. Nakamura, K.; Fujita, Y.; Itoh, Y.; Yamane, K. Modification of length, hydrophobic properties and electric charge of bacillus subtilis α-amylase signal peptide and their different effects on the production of secretory proteins in B. subtilis and E. coli cells. Mol. Gen. Genet. 1989, 216, 1-7.
3. Yatvin, M. B.; Smith, K. M.; Siegel, F. L. Translocation of nascent nonsignal sequence protein in heated Escherichia coli. J. Biol. Chem. 1986, 261, 8070-8075.
4. Ingram, L. O. Mechanism of lysis of Escherichia coli by ethanol and other chaotropic agents, J. Bacteriol. 1981, 46, 331-336.
5. DeSmet, M. J.; Kingma, J.; Witholt, B. The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli. Biochim. Biophys. Acta 1978, 506, 64-80.
6. Woldringh, C. L. Lysis of the cell membrane of Escherichia coli K12 by ionic detergent. Biochim. Biophys. Acta 1970, 224, 288-290.
7. Ellis, R. J.; Van der Vies, S. M. Molecular chaperones. Annu. Rev. Biochim.; 1976, 72, 321-347.
8. Goldberg, A. L.; Dice, J. F. Intracellular protein degradation in mammalian and bacterial cells. Annu. Rev. Biochim. 1974, 34, 835-869.
9. Georgoppulos, C.; Welch, W. J., Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell. Biol. 1993, 601-634.
10. Kitagawa, M.; Matsumura, Y.; Tsuchido, T. Heat induced translocation and aggregation of cytoplasmic proteins in Escherichia coli cells. Submitted to Mol. Microbiol.
11. Umakoshi, H.; Kuboi, R.; Komasawa, I.; Tsuchido, T.; Matsumura, Y. Heat-induced translocation of cytoplasmic β-galactosidase across inner membrane of Escherichia coli. Biotechnol. Prog. 1998, 14, 210-217.
12. Umakoshi, H.; Yoshimoto, M.; Shimanouchi, T.; Kuboi, R.; Komasawa, I. Model system for heat-induced translocation of β-galactosidase across phospholipid membrane. Biotechnol. Prog. 1998, 14, 218-226.
13. Umakoshi, H.; Shimanouchi, T.; Kuboi, R. Selective separation using heat-induced translocation of proteins across phospholipid membrane. J. Chromatogr. B 1998, 711, 111-116.
14. Schnaitman, C. A. Solublization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J. Bacteriol. 1971, 108, 545-552.
15. Asenjo, J. A.; Andrews, B. A. Enzymatic cell lysis for product release. In Separation Process in Biotechnology; Asenjo, J. A., Eds.; Marcel Dekker: New York, 1990; pp 143-175.
16. Umakoshi, H.; Kuboi, R.; Komasawa, I. Design of selective recovery of enzymes from Escherichia coli based on their location using nonionic detergent, Triton X-series. Trans. Inst. Chem. Eng., Part C 1998, 76, 162-168.
17. Umakoshi, H.; Kuboi, R. Enzyme release from heat-stressed cell membranes as a function of hydrophobicity evaluated by using aqueous two-phase systems. J. Chromatogr. B 1998, 711, 217-222.
18. Steers, E., Jr.; Cuatrecasas, P.; Pollard, H. B. The purification of β-galactosidase from Escherichia coli by affinity chromatography. J. Biol. Chem. 1971, 246 (1), 196-200.
19. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1975, 72, 248-254.
20. Kuboi, R.; Yamahara, K.; Ota, H. Evaluation of denaturation and aggregation formation of enzyme under heat stress conditions. J. Chem. Eng. Jpn. 1997, 30 (6), 7-10.