A novel strategy for generation of monoclonal antibodies from single B cells using RT-PCR technique and in vitro expression

Biotechnology Progress

Volumn 22, Issue 4, 2006, Pages 979-988

  Jiang, XiuPing ^a,b,d   Suzuki, Hirotatsu ^a,b   Hanai, Yuko ^a,c   Wada, Fumitaka ^a,c   Hitomi, Kiyotaka ^a,c   Yamane, Tsuneo ^a,b,e   Nakano, Hideo ^a,b  

^a NAGOYA UNIVERSITY   (Japan)

^b Laboratory of Molecular Biotechnology   (Japan)

^c Laboratory of Molecular and Cellular Regulation   (Japan)

^d INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^e CHUBU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODIES (MABS); PERIPHERAL BLOOD; SINGLE-CELL RT-PCR-LINKED IN VITRO EXPRESSION (SICREX);

BLOOD; CELLS; DNA; GENES; IMMUNOLOGY;

ANTIBODIES;

IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL; B LYMPHOCYTE; BAGG ALBINO MOUSE; BIOSYNTHESIS; CELL SEPARATION; CONFERENCE PAPER; CYTOLOGY; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PROTEIN ENGINEERING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPLEEN;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; B-LYMPHOCYTES; BASE SEQUENCE; CELL SEPARATION; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPLEEN;

ANIMALIA; ESCHERICHIA COLI;

EID: 33747167351     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp060092h     Document Type: Conference Paper

References (19)

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