PCR-based generation of shRNA libraries from cDNAs

BMC Biotechnology

Volumn 6, Issue , 2006, Pages

  Du, Cheng ^a   Ge, Baosheng ^a   Liu, Zhongfeng ^a   Chan, Wing C ^a   McKeithan, Timothy W ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA FRAGMENTATION; SHORT HAIRPIN RNA LIBRARIES;

BIOSYNTHESIS; CLONING; DNA; ENZYME KINETICS; MUTAGENESIS; RNA;

BIOTECHNOLOGY;

COMPLEMENTARY DNA; DNA FRAGMENT; DOUBLE STRANDED DNA; ENDONUCLEASE; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; OLIGONUCLEOTIDE; SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CASSETTE; GENE EXPRESSION; GENE MUTATION; GENE STRUCTURE; GENE TARGETING; GENETIC ANALYSIS; GENETIC TRANSFECTION; GENOME; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MOLECULAR CLONING; MOUSE; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; RNA INTERFERENCE; SYNTHESIS;

CLONING, MOLECULAR; DNA FRAGMENTATION; DNA RESTRICTION ENZYMES; GENE LIBRARY; POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING;

MAMMALIA;

EID: 33747024517     PISSN: 14726750     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-6-28     Document Type: Article

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