Fluorescent styryl dyes FM1-43 and FM2-10 are muscarinic receptor antagonists: Intravital visualization of receptor occupancy

Journal of Physiology

Volumn 575, Issue 1, 2006, Pages 23-35

  Mazzone, Stuart B ^a   Mori, Nanako ^b   Burman, Miriam ^c   Palovich, Michael ^c   Belmonte, Kristen E ^c   Canning, Brendan J ^b  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b JOHNS HOPKINS ASTHMA AND ALLERGY CENTER   (United States)

^c GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 DIPHENYLACETOXY 1 METHYLPIPERIDINE; ATROPINE; BROMINE DERIVATIVE; FLUORESCENT DYE; FM 1 43; FM 2 10; HISTAMINE H1 RECEPTOR; METHOCTRAMINE; MUSCARINIC M2 RECEPTOR ANTAGONIST; MUSCARINIC M3 RECEPTOR ANTAGONIST; MUSCARINIC RECEPTOR; MUSCARINIC RECEPTOR BLOCKING AGENT; N (3 TRIETHYLAMMONIUMPROPYL) 4 [4 (DIBUTYLAMINO)STYRYL]PYRIDINIUM DIBROMIDE; N (3 TRIETHYLAMMONIUMPROPYL) 4 [4 (DIETHYLAMINO)STYRYL]PYRIDINIUM DIBROMIDE; NEUROKININ 2 RECEPTOR; OMEGA CONOTOXIN GVIA; PIRENZEPINE; RADIOLIGAND; TETRODOTOXIN; UNCLASSIFIED DRUG; MUSCARINIC M1 RECEPTOR; MUSCARINIC M2 RECEPTOR; MUSCARINIC M3 RECEPTOR; MUSCARINIC M4 RECEPTOR; MUSCARINIC M5 RECEPTOR; TACHYKININ RECEPTOR;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CELL LABELING; CHO CELL; CHOLINERGIC STIMULATION; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG STRUCTURE; ELECTROSTIMULATION; ENDOCYTOSIS; EXOCYTOSIS; GUINEA PIG; IN VITRO STUDY; LIGAND BINDING; MALE; MOLECULAR CLONING; NERVE ENDING; NEUROTRANSMISSION; NEUROTRANSMITTER RELEASE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR OCCUPANCY; REGULATORY MECHANISM; SMOOTH MUSCLE CONTRACTION; SMOOTH MUSCLE RELAXATION; TRACHEA MUSCLE; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); ELECTRIC FIELD; INCUBATION TIME; MOLECULAR IMAGING; RADIOASSAY;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DOSE-RESPONSE RELATIONSHIP, DRUG; FLUORESCENT DYES; GUINEA PIGS; MALE; MUSCARINIC ANTAGONISTS; MUSCLE CONTRACTION; MUSCLE, SMOOTH; PYRIDINIUM COMPOUNDS; QUATERNARY AMMONIUM COMPOUNDS; RECEPTORS, MUSCARINIC; STAINING AND LABELING; TRACHEA; TRANSFECTION;

EID: 33746806221     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2006.106351     Document Type: Article

References (41)

1. Ashe JH & Yarosh CA (1984). Differential and selective antagonism of the slow inhibitory postsynaptic potential and slow-excitatory postsynaptic potential by gallamine and pirenzepine in the superior cervical ganglion of the rabbit. Neuropharmacology 23, 1321-1329.
2. Belmonte KE, McKinnon LA & Nathanson NM (2000). Developmental expression of muscarinic acetylcholine receptors in chick retina: selective induction of M2 muscarinic receptor expression in ovo by a factor secreted by muller glial cells. J Neurosci 20, 8417-8425.
3. 2+ current and M current in rat sympathetic neurons. Proc Natl Acad Sci U S A 89, 9544-9548.
4. Bewick GS & Betz WJ (1994). Illumination partly reverses the postsynaptic blockade of the frog neuromuscular junction by the styryl pyridinium dye RH414. Proc R Soc Lond B Biol Sci 258, 201-207.
5. Buckley NJ, Bonner TI, Buckley CM & Brann MR (1989). Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells. Mol Pharmacol 35, 469-476.
6. Canning BJ & Fischer A (2001). Neural regulation of airway smooth muscle tone. Respir Physiol 25, 113-127.
7. Cheng Y-C & Prusoff WH (1973). Relationship between the inhibition constant (K i) and the concentration of inhibitor which cause 50 percent inhibition (IC 50) of an enzymatic reaction. Biochem Pharmacol 22, 3099-3108.
8. Dawkins R, Keller SL & Sewell WF (2005). Pharmacology of acetylcholine-mediated cell signaling in the lateral line organ following efferent stimulation. J Neurophysiol 93, 2541-2545.
9. Eglen RM, Choppin A, Watson N & Hegde SS (1999). Muscarinic receptor assays. In Current Protocols in Pharmacology ed. Enna SJ, William M, Barrett JF, Ferkany JW, Kenakin T & Porsolt RD, pp. 4.15.1-4.15.20. John Wiley and Sons, USA.
10. Fisher JT, Vincent SG, Gomeza J, Yamada M & Wess J (2004). Loss of vagally mediated bradycardia and bronchoconstriction in mice lacking M2 or M3 muscarinic acetylcholine receptors. FASEB J 18, 711-713.
11. Fryer AD & Jacoby DB (1998). Muscarinic receptors and control of airway smooth muscle. Am J Respir Crit Care Med 158, S154-S160.
12. Gale JE, Marcotti W, Kennedy HJ, Kros CJ & Richardson GP (2001). FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel. J Neurosci 21, 7025.
13. Grinvald A, Lieke E, Frostig RD, Gilbert CD & Weisel TN (1986). Functional architecture of cortex revealed by optical imaging of intrinsic signals. Nature 324, 361-364.
14. Haddad EB, Landry Y & Gies JP (1991). Muscarinic receptor subtypes in guinea-pig airways. Am J Physiol 261, L327-L333.
15. Hay M & Hasser EM (1998). Measurement of synaptic vesicle exocytosis in aortic baroreceptor neurons. Am J Physiol 275, H710-H716.
16. Iannazzo L & Majewski H (2000). M2/M4-muscarinic receptors mediate automodulation of acetylcholine outflow from the mouse cortex. Neurosci Lett 287, 129-132.
17. Jaarsma D, Ruigrok TJ, Caffe R, Cozzari C, Levey AI, Mugnaini E & Voogd J (1997). Cholinergic innervation and receptors in the cerebellum. Prog Brain Res 114, 67-96.
18. Kay AR, Alfonso A, Alford S, Cline HT, Holgado AM, Sakmann B, Snitsarev VA, Stricker TP, Takahashi M & Wu L-G (1999). Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey and rat. Neuron 24, 809-817.
19. Khan KM, Drescher MJ, Hatfield JS, Khan AM & Drescher DG (2002). Muscarinic receptor subtypes are differentially distributed in the rat cochlea. Neuroscience 111, 291-302.
20. Kirchgessner AL, Liu M-T & Gershon MD (1996). In situ identification and visualization of neurons that mediate enteric and enteropancreatic reflexes. J Comp Neurol 371, 270-286.
21. Krejci A & Tucek S (2001). Changes of cooperativity between N-methylscopolamine and allosteric modulators alcuronium and gallamine induced by mutations of external loops of muscarinic M3 receptors. Mol Pharm 60, 761-767.
22. Levey AI (1993). Immunological localization of m1-m5 muscarinic acetylcholine receptors in peripheral tissues and brain. Life Sci 52, 441-448.
23. Martin CA, Naline E, Emonds-Alt X & Advenier C (1992). Influence of (+/-)-CP-96,345 and SR 48968 on electrical field stimulation of the isolated guinea-pig main bronchus. Eur J Pharmacol 224, 137-143.
24. Meyers JR, MacDonald RB, Duggan A, Lenzi D, Standaert DG, Corwin JT & Corey DP (2003). Lighting up the senses: FM1-43 loading of sensory cells through nonselective ion channels. J Neurosci 23, 4054-4065.
25. Myers AC & Undem BJ (1996). Muscarinic receptor regulation of synaptic transmission in airway parasympathetic ganglia. Am J Physiol 270, L630-L636.
26. Pyle JL, Kavalali ET, Choi S & Tsien RW (1999). Visualization of synaptic activity in hippocampal slices with FM1-43 enabled by fluorescence quenching. Neuron 24, 803-808.
27. Ribchester RR, Mao F & Betz WJ (1994). Optical measurements of activity-dependent membrane recycling in motor nerve terminals of mammalian skeletal muscle. Proc R Soc Lond B Biol Sci 255, 61-66.
28. Rizzoli SO, Richards DA & Betz WJ (2003). Monitoring synaptic vesicle recycling in frog motor nerve terminals with FM dyes. J Neurocytol 32, 539-549.
29. Ryan TA, Reuter H, Wendland B, Schweizer FE, Tsien RW & Smith SJ (1993). The kinetics of synaptic vesicle recycling measured at single presynaptic boutons. Neuron 11, 713-724.
30. Santafe MM, Salon I, Garcia N, Lanuza MA, Uchitel OD & Tomas J (2003). Modulation of ACh release by presynaptic muscarinic autoreceptors at the neuromuscular junction of the newborn and adult rat. Eur J Neurosci 17, 119-127.
31. Slutsky I, Wess J, Gomeza J, Dudel J, Parnas I & Parnas H (2003). Use of knockout mice reveals involvement of M2-muscarinic receptor control of the kinetics of acetylcholine release. J Neurophysiol 89, 1954-1967.
32. Smith JC, Cavallito CJ & Foldes FF (1967). Choline acetyltransferase inhibitors: A group of styryl-pyridine analogs. Biochem Pharmacol 16, 2438-2441.
33. Struckmann N, Schwering S, Wiegand S, Gschnell A, Yamada M, Kummer W, Wess J & Haberberger RV (2003). Role of muscarinic receptor subtypes in the constriction of peripheral airways: Studies on receptor-deficient mice. Mol Pharmacol 64, 1444-1451.
34. Tanczos AC, Palmer RA, Potter BS, Saldanha JW & Howlin BJ (2004). Antagonist binding in the rat muscarinic receptor A study by docking and X-ray crystallography. Comput Biol Chem 28, 375-385.
35. Torneke K, Ingvast-Larsson C, Bostrom A & Appelgren LE (2002). Muscarinic receptors in equine airways. Vet Res Commun 26, 637-650.
36. Trendelenburg AU, Gomeza J, Klebroff W, Zhou H & Wess J (2003). Heterogeneity of presynaptic muscarinic receptors mediating inhibition of sympathetic transmitter release: A study with M2- and M4-receptor deficient mice. Br J Pharmacol 138, 469-480.
37. Van der Zee EA & Luiten PG (1999). Muscarinic receptors in the hippocampus, neocortex and amygdale: A review of immunocytochemical localization in relation to learning and memory. Prog Neurobiol 58, 409-471.
38. Yang Z-J & Biggs DF (1991). Muscarinic receptors and parasympathetic neurotransmission in guinea-pig trachea. Eur J Pharmacol 193, 301-308.
39. Zaagsma J, Roffel AF & Meurs H (1997). Muscarinic control of airway function. Life Sci 60, 1061-1068.
40. Zhang W, Basile AS, Gomeza J, Volpicelli LA, Levey AI & Wess J (2002). Characterization of central inhibitory muscarinic autoreceptors by the use of muscarinic acetylcholine receptor knockout mice. J Neurosci 22, 1709-1717.
41. Zhang W & Warren RA (2002). Muscarinic and nicotinic presynaptic modulation of EPSCs in the nucleus accumbens during postnatal development. J Neurophysiol 88, 3315-3330.