Monitoring of the succession of bacterial populations in infant faeces by PCR-denaturing gradient gel electrophoresis

Microbial Ecology in Health and Disease

Volumn 17, Issue 4, 2005, Pages 205-211

  Jose Pozuelo De Felipe M ^a   García Albiach, Raimundo ^a   Montesi Libois, Alejandra ^a   Rodríguez Borrajo, Coronación ^a   Del Campo, Rosa ^b   Rotger, Rafael ^c  

^a UNIVERSIDAD SAN PABLO CEU   (Spain)

^b HOSPITAL RAMÓN Y CAJAL   (Spain)

^c UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Bifidobacterium; DGGE; Faeces; Infant nutrition; Lactobacillus; Microbiology

Indexed keywords

RIBOSOME DNA;

ARTICLE; BACTERIAL FLORA; BIFIDOBACTERIUM; BREAST MILK; DENATURING GRADIENT GEL ELECTROPHORESIS; FECES; GENE AMPLIFICATION; HUMAN; INFANT; LACTOBACILLUS; NONHUMAN; NORMAL HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPECIES DOMINANCE;

BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; LACTOBACILLUS;

EID: 33746576873     PISSN: 0891060X     EISSN: 16512235     Source Type: Journal    
DOI: 10.1080/08910600600600158     Document Type: Article

References (32)

1. Guarner F, Malagelada JR. Gut flora in health and disease. Lancet. 2003;361:512-9.
2. Mackie RI, Sghir A, Gaskins HR. Developmental microbial ecology of the neonatal gastrointestinal tract. Am J Clin Nutr. 1999;69:1035S-1045.
3. Orrhage K, Nord CE. Factors controlling the bacterial colonization of the intestine in breastfed infants. Acta Paediatr Suppl. 1999;88:47-57.
4. Hopkins MJ, Sharp R, Macfarlane GT. Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles. Gut. 2001;48:198-205.
5. Schwiertz A, Gruhl B, Löbnitz M, Michel P, Radke M, Blaut M. Development of the intestinal bacterial composition in hospitalized preterm infants in comparison with breast-fed, full-term infants. Pediatr Res. 2003;54:393-9.
6. Suau A, Bonnet R, Sutren M, Godon JJ, Gibson GR, Collins MD, et al. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol. 1999;65:4799-807.
7. Sakata S, Tonooka T, Ishizeki S, Takada M, Sakamoto M, Fukuyama M, et al. Culture-independent analysis of fecal microbiota in infants, with special reference to Bifidobacterium species. FEMS Microbiol Lett. 2005;243:417-23.
8. Muyzer G, de Waal A, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol. 1993;59:695-700.
9. Zoetendal EG, Akkermans AD, de Vos WM. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol. 1998;64:3854-9.
10. Vanhoutte T, Huys G, De Brandt E, Swings J. Temporal stability analysis of the microbiota in human feces by denaturing gradient gel electrophoresis using universal and group-specific 16S rRNA gene primers. FEMS Microbiol Ecol. 2004;48:437-46.
11. Muyzer G, Hottenträger A, Teske A, Waver A. Denaturing gradient gel electrophoresis of PCR amplified 16S rDNA - a new molecular approach to analyze the genetic diversity of mixed microbial communities. In: Akkermans A, Van Elsas J, de Bruijn F. editors. Molecular microbial ecology manual. Dordrecht: Kluwer Academic Publishers; 1996. p. 1-23.
12. Zoetendal EG, Collier CT, Koike S, Mackie RI, Gaskins HR. Molecular ecological analysis of the gastrointestinal microbiota: a review. J Nutr. 2004;134:465-72.
13. Favier CF, Vaughan EE, de Vos WM, Akkermans AD. Molecular monitoring of succession of bacterial communities in human neonates. Appl Environ Microbiol. 2002;68:219-26.
14. Favier CF, de Vos WM, Akkermans ADL. Development of bacterial and bifidobacterial communities in feces of newborn babies. Anaerobe. 2003;9:219-29.
15. Wang M, Ahrne S, Antonsson M, Molin G. T-RFLP combined with principal component analysis and 16S rRNA gene sequencing: an effective strategy for comparison of fecal microbiota in infants of different ages. Gastroenterol Hepatol. 2004;59:53-69.
16. Haarman M, Knol J. Quantitative real-time per assays to identify and quantify fecal bifidobacterium species in infants receiving a prebiotic infant formula. Appl Environ Microbiol. 2005;71:2318-24.
17. Penders J, Vink C, Driessen C, London N, Thijs C, Stobberingh EE. Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR. FEMS Microbiol Lett. 2005;243:141-7.
18. Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP. Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol. 2001;67:2578-85.
19. Satokari RM, Vaughan EE, Akkermans ADL, Saarela M, de Vos WM. Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis. Appl Environ Microbiol. 2001;67:504-13.
20. Montesi A, Garcia-Albiach R, Pozuelo MJ, Pintado C, Goñi I, Rotger R. Molecular and microbiological analysis of caecal microbiota in rats fed with diets supplemented either with prebiotics or probiotics. Int J Food Microbiol. 2005;98:281-9.
21. Donskey CJ, Hujer AM, Das SM, Pultz NJ, Bonomo RA, Rice LB. Use of denaturing gradient gel electrophoresis for analysis of the stool microbiota of hospitalized patients. Gastroenterol Hepatol. 2003;54:249-56.
22. Sanguinetti CJ, Dias Neto E, Simpson AJ. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques. 1994;17:914-21.
23. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, et al. Current protocols in molecular biology. New York: Greene Publishing Associates and Wiley Interscience; 1993.
24. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, et al. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol. 2000;66:297-303.
25. Temmerman R, Huys G, Swings J. Identification of lactic acid bacteria: culture-dependent and culture-independent methods. Trends Food Sci Technol. 2004;15:348-59.
26. Gavini F, Cayuela C, Antoine J-M, Lecoq C, Lefebvre B, Membré J-M, et al. Differences in the distribution of bifidobacterial and enterobacterial species in human faecal microflora of three different (children, adults, elderly) age groups. Microb Ecol Health Dis. 2001;13:40-5.
27. Heilig HGHJ, Zoetendal EG, Vaughan EE, Marteau P, Akkermans ADL, de Vos WM. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol. 2002;68:114-23.
28. Satokari RM, Vaughan EE, Favier CF, Doré J, Edwards C, de Vos WM. Diversity of Bifidobacterium and Lactobacillus spp. in breast-fed and formula-fed infants as assessed by 16S rDNA sequence differences. Microb Ecol Health Dis. 2001;14:97-105.
29. Sghir A, Gramet G, Suau A, Rochet V, Pochart P, Dore J. Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization. Appl Environ Microbiol. 2000;66:2263-6.
30. Mevissen-Verhage EA, Marcelis JH, de Vos WM, Harmsen-van Amerongen WC, Verhoef J. Bifidobacterium, Bacteroides, and Clostridium spp. in fecal samples from breast-fed and bottle-fed infants with and without iron supplement. J Clin Microbiol. 1987;25:285-9.
31. Mullie C, Odou MF, Singer E, Romond MB, Izard D. Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human origin. FEMS Microbiol Lett. 2003;222:129-36.
32. Nielsen DS, Moller PL, Rosenfeldt V, Paerregaard A, Michaelsen KF, Jakobsen M. Case study of the distribution of mucosa-associated Bifidobacterium species, Lactobacillus species, and other lactic acid bacteria in the human colon. Appl Environ Microbiol. 2003;69:7545-8.