Novel hydroxyl radical scavenging antioxidant activity assay for water-soluble antioxidants using a modified CUPRAC method

Biochemical and Biophysical Research Communications

Volumn 345, Issue 3, 2006, Pages 1194-1200

  Bektaşoǧlu, Burcu ^a   Esin Çelik, Saliha ^a   Özyürek, Mustafa ^a   Güçlü, Kubilay ^a   Apak, Reşat ^a  

^a ISTANBUL UNIVERSITY   (Turkey)

Author keywords

Antioxidant activity; CUPRAC method; Hydroxyl radical scavenging; Spectrophotometry; Water soluble antioxidants

Indexed keywords

2,9 DIMETHYL 1,10 PHENANTHROLINE; 4 AMINOBENZOIC ACID; ACETIC ACID ETHYL ESTER; ALCOHOL DERIVATIVE; ANTIOXIDANT; ASCORBIC ACID; BENZOIC ACID DERIVATIVE; CUPRIC ION; DIMETHYL SULFOXIDE; EDETIC ACID; FERROCYANIDE; FERROUS ION; FORMIC ACID; GLUCOSE; HYDROGEN PEROXIDE; HYDROXYL GROUP; HYDROXYL RADICAL; IODIDE; LYSINE; MANNITOL; PEROXY RADICAL; REACTIVE OXYGEN METABOLITE; SCAVENGER; SODIUM METABISULFITE; SUPEROXIDE; THIOBARBITURIC ACID REACTIVE SUBSTANCE; THIOUREA; FREE RADICAL;

ANTIOXIDANT ACTIVITY; ARTICLE; COLOR VISION; ELECTROCHEMICAL DETECTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; INCUBATION TIME; MACROMOLECULE; MATHEMATICAL MODEL; MOLECULAR PROBE; OXIDATIVE STRESS; PRIORITY JOURNAL; SCAVENGING SYSTEM; SENSITIVITY ANALYSIS; BIOCHEMISTRY; CHEMISTRY; ELECTROCHEMISTRY; KINETICS; METABOLISM; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

ANTIOXIDANTS; ASCORBIC ACID; BIOCHEMISTRY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; EDETIC ACID; ELECTROCHEMISTRY; FREE RADICAL SCAVENGERS; FREE RADICALS; HYDROGEN PEROXIDE; HYDROXYL RADICAL; KINETICS; REACTIVE OXYGEN SPECIES; SENSITIVITY AND SPECIFICITY; THIOBARBITURIC ACID REACTIVE SUBSTANCES;

EID: 33746561383     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.05.038     Document Type: Article

References (30)

1. Halliwell B., and Gutteridge J.M.C. Free Radicals in Biology and Medicine (1989), Oxford University Press, Oxford
2. Halliwell B., and Auroma O.I. DNA damage by oxygen-derived species: its mechanism and measurements in mammalian systems. FEBS Lett. 281 (1991) 9-19
3. Halliwell B., and Gutteridge J.M.C. Biologically relevant metal ion-dependent hydroxyl radical generation. An update. FEBS Lett. 307 (1992) 108-112
4. Halliwell B., and Gutteridge J.M.C. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 219 (1984) 1-14
5. Halliwell B., and Gutteridge J.M.C. Oxygen free radical and iron in relation to biology and medicine: some problems and concepts. Arch. Biochem. Biophys. 246 (1986) 501-514
6. Chen S.-X., and Schopfer P. Hydroxyl-radical production in physiological reactions. A novel function of peroxidase. Eur. J. Biochem. 260 (1999) 726-735
7. Gutteridge J.M.C. Ferrous-salt-promoted damage to deoxyribose and benzoate. The increased effectiveness of hydroxyl-radical scavengers in the presence of EDTA. Biochem. J. 243 (1987) 709-714
8. Gutteridge J.M.C. Thiobarbituric acid-reactivity following iron-dependent free-radical damage to amino acids and carbohydrates. FEBS Lett. 128 (1981) 343-346
9. Halliwell B., and Gutteridge J.M.C. Formation of a thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts. The role of superoxide and hydroxyl radicals. FEBS Lett. 128 (1981) 347-352
10. Aruoma O.I., Grootveld M., and Halliwell B. The role of iron in ascorbate-dependent deoxyribose degradation-evidence consistent with a site-specific hydroxyl radical generation caused by iron ions bound to the deoxyribose molecule. J. Inorg. Biochem. 29 (1987) 289-299
11. Halliwell B., Gutteridge J.M., and Aruoma O.I. The deoxyribose method: a simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165 (1987) 215-219
12. Buege J.A., and Aust S.D. Microssomal lipid peroxidation. Methods Enzymol. 52 (1978) 302-310
13. Cheng Z., Li Y., and Chang W. Kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a Fenton-type reaction system. Anal. Chim. Acta 478 (2003) 129-137
14. Halliwell B., Grootveld M., and Gutteridge J.M.C. Methods for the measurement of hydroxyl radicals in biological systems: deoxyribose degradation and aromatic hydroxylation. Methods Biochem. Anal. 33 (1988) 59-90
15. Grootveld M., and Halliwell B. Aromatic hydroxylation as a potential measure of hydroxyl-radical formation in vivo. Identification of hydroxylated derivatives of salicylate in human body fluids. Biochem. J. 237 (1986) 499-504
16. Singh S., and Hider R.C. Colorimetric detection of the hydroxyl radical: comparison of the hydroxyl-radical-generating ability of various iron complexes. Anal. Biochem. 171 (1988) 47-54
17. Apak R., Güçlü K., Özyürek M., and Karademir S.E. A novel total antioxidant capacity index for dietary polyphenols, vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem. 52 (2004) 7970-7981
18. Apak R., Güçlü K., Özyürek M., Karademir S.E., and Altun M. Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: the CUPRAC method. Free Radic. Res. 39 (2005) 949-961
19. McHatton R.C., Espenson J.H., and Bakac A. Carbon-carbon bond formation in the reaction of aliphatic radicals with alkylcobaloximes. J. Am. Chem. Soc. 108 (1986) 5885-5890
20. Pronai L., Ichikawa Y., Ichimori K., Nakazawa H., and Arimori S. Hydroxyl radical-scavenging activity of slow-acting anti-rheumatic drugs. J. Clin. Biochem. Nutr. 9 (1990) 17-23
21. Aruoma O.I., Halliwell B., Hoey B.M., and Butler J. The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem. J. 256 (1988) 251-255
22. Halliwell B. Superoxide-dependent formation of hydroxyl radicals in the presence of iron chelates. Is it a mechanism for hydroxyl radical production in biochemical systems?. FEBS Lett. 92 (1978) 321-326
23. -) in aqueous solution. J. Phys. Chem. Ref. Data 17 (1988) 513-886
24. Aruoma O.I., Laughton M.J., and Halliwell B. Carnosine, homocarnosine and anserine: could they act as antioxidants in vivo?. Biochem. J. 264 (1989) 863-869
25. Tutem E., Apak R., and Baykut F. Spectrophotometric determination of trace amounts of copper(I) and reducing agents with neocuproine in the presence of copper(II). Analyst 116 (1991) 89-94
26. Aruoma O.I., Halliwell B., Gajewski E., and Dizdaroglu E. Deoxyribose assay for detecting hydroxyl radicals. J. Biol. Chem. 264 (1989) 57-66
27. Ghiselli A. Aromatic hydroxylation. Salicylic acid as a probe for measuring hydroxyl radical production. Methods Mol. Biol. 108 (1998) 89-100
28. Chan T.S., Galati G., Pannala A.S., Rice-Evans C., and O'Brien P.J. Simultaneous detection of the antioxidant and pro-oxidant activity of dietary polyphenolics in a peroxidase system. Free Radic. Res. 37 (2003) 787-794
29. Ou B., Hampsch-Woodill M., Flanagan J., Deemer E.K., Prior R.L., and Huang D. Novel fluorometric assay for hydroxyl radical prevention capacity using fluorescein as the probe. J. Agric. Food Chem. 50 (2002) 2772-2777
30. Ou B., Hampsch-Woodill M., and Prior R.L. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J. Agric. Food Chem. 49 (2001) 4619-4626