Nourseothricin acetyltransferease: A positive selectable marker for Toxoplasma gondii

Journal of Parasitology

Volumn 92, Issue 3, 2006, Pages 668-670

  Van, Tam T ^a   Rooney, Peggy J ^a   Knoll, Laura J ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; BIOLOGICAL MARKER; NOURSEOTHRICIN; NOURSEOTHRICIN ACETYLTRANSFERASE; UNCLASSIFIED DRUG;

ENZYME; GENETIC MARKER; GENOME; MOLECULAR ANALYSIS; PARASITE; PROTOZOAN;

ARTICLE; ELECTROPORATION; EXPRESSED SEQUENCE TAG; GENE; GENOME; NAT1 GENE; NONHUMAN; OPEN READING FRAME; PARASITE; TOXOPLASMA GONDII;

ACETYLTRANSFERASES; ANIMALS; BLOTTING, SOUTHERN; DRUG RESISTANCE; ELECTROPORATION; GENETIC MARKERS; HUMANS; MALE; STREPTOMYCES; STREPTOTHRICINS; TOXOPLASMA; TRANSFORMATION, GENETIC;

STREPTOMYCES NOURSEI; TOXOPLASMA GONDII;

EID: 33746518523     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.1645/GE-706R.1     Document Type: Article

References (17)

1. BLACK, M., F. SEEBER, D. SOLDATI, K. KIM, AND J. C. BOOTHKOYD. 1995. Restriction enzyme-mediated integration elevates transformation frequency and enables co-transfection of Toxoplasma gondii. Molecular and Biochemical Parasitology 74: 55-63.
2. BRECHT, S., H. ERDHART, M. SOETE, AND D. SOLDATI. 1999. Genome engineering of Toxoplasma gondii using the site-specific recombinase Cre. Gene 234: 239-247.
3. DONALD, R. G., D. CARTER, B. ULLMAN, AND D. S. ROOS. 1996. Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine- guanine phosphoribosyltransferase gene. Use as a selectable marker for stable transformation. Journal of Biological Chemistry 271: 14010-14019.
4. -, AND D. S. ROOS. 1993. Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proceedings of the National Academy of Sciences USA 90: 11703-11707.
5. -, AND -. 1998. Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for hit-and-run mutagenesis. Molecular and Biochemical Parasitology 91: 295-305.
6. GOLDSTEIN, A. L., AND J. H. MCCUSKER. 1999. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541-1553.
7. JOSHI, P. B., J. R. WEBB, J. E. DAVIES, AND W. R. MCMASTER. 1995. The gene encoding streptothricin acetyltransferase (sat) as a selectable marker for Leishmania expression vectors. Gene 156: 145-149.
8. KIM, K., M. S. EATON, W. SCHUBERT, S. WU, AND J. TANG. 2001. Optimized expression of green fluorescent protein in Toxoplasma gondii using thermostable green fluorescent protein mutants. Molecular and Biochemical Parasitology 113: 309-313.
9. -, D. SOLDATI, AND J. C. BOOTHROYD. 1993. Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science 262: 911-914.
10. KNOLL, L. J., AND J. C. BOOTHROYD. 1998. Isolation of developmentally regulated genes from Toxoplasma gondii by a gene trap with the positive and negative selectable marker hypoxanthine-xanthine-guanine phosphoribosyltransferase. Molecular and Cellular Biology 18: 807-814.
11. MCDADE, H. C., AND G. M. COX. 2001. A new dominant selectable marker for use in Cryptococcus neoformans. Medical Mycology 39: 151-154.
12. MEISSNER, M., D. SCHLUTER, AND D. SOLDATI. 2002. Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion. Science 298: 837-840.
13. MESSINA, M., I. NIESMAN, C. MERCIER, AND L. D. SIBLEY. 1995. Stable DNA transformation of Toxoplasma gondii using phleomycin selection. Gene 165: 213-217.
14. PFEFFERKORN, E. R., AND L. C. PFEFFERKORN. 1977. Specific labeling of intracellular Toxoplasma gondii with uracil. Journal of Protozoology 24: 449-453.
15. SHEN, J., W. GUO, AND J. R. KOHLER. 2005. CaNAT1, a heterologous dominant selectable marker for transformation of Candida albicans and other pathogenic Candida species. Infection and Immunity 73: 1239-1242.
16. SIBLEY, L. D., M. MESSINA, AND I. R. NIESMAN. 1994. Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy. Proceedings of the National Academy of Sciences USA 91: 5508-5512.
17. SOLDATI, D., AND J. C. BOOTHROYD. 1993. Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii. Science 260: 349-352.