Cytotoxicity of pharmaceuticals found in aquatic systems: Comparison of PLHC-1 and RTG-2 fish cell lines

Aquatic Toxicology

Volumn 79, Issue 2, 2006, Pages 114-123

  Caminada, Daniel ^a,b   Escher, Claudia ^c   Fent, Karl ^a,c  

^a UNIVERSITY OF APPLIED SCIENCES AND ARTS NORTHWESTERN SWITZERLAND   (Switzerland)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c ETH ZURICH   (Switzerland)

Author keywords

Aquatic environment; Comparison in vitro in vivo; Cytotoxicity; Fish cell lines; Pharmaceuticals

Indexed keywords

ANALGESIC AGENT; ANTIINFLAMMATORY AGENT; ANTILIPEMIC AGENT; ATENOLOL; ATORVASTATIN; BETA ADRENERGIC RECEPTOR BLOCKING AGENT; BEZAFIBRATE; CARBAMAZEPINE; CENTRAL NERVOUS SYSTEM AGENTS; CIMETIDINE; CLOFIBRIC ACID; DIAZEPAM; DICLOFENAC; DOXORUBICIN; DYE; FENOFIBRATE; FLUOXETINE; FUROSEMIDE; IBUPROFEN; MEFENAMIC ACID; METFORMIN; METHOTREXATE; METOPROLOL; NAPROXEN; NEUTRAL RED; PROPRANOLOL; RANITIDINE; SALICYLIC ACID; THIAZOLYL BLUE TETRAZOLIUM BROMIDE; UNCLASSIFIED DRUG; UNINDEXED DRUG;

AQUATIC ENVIRONMENT; DRUG; ECOTOXICOLOGY; FISH;

ACUTE TOXICITY; ANIMAL CELL; AQUATIC ENVIRONMENT; ARTICLE; BIOASSAY; CELL LINE; COMPARATIVE STUDY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOTOXICITY; CYTOTOXICITY TEST; ECOTOXICITY; HORMESIS; IN VITRO STUDY; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; PH MEASUREMENT; PRIORITY JOURNAL;

ANIMALS; CELL LINE; CYPRINODONTIFORMES; CYTOTOXINS; DRUG TOXICITY; ENVIRONMENTAL EXPOSURE; FRESH WATER; ONCORHYNCHUS MYKISS; PHARMACEUTICAL PREPARATIONS; TOXICITY TESTS; WATER POLLUTANTS, CHEMICAL;

DAPHNIA;

EID: 33746350457     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aquatox.2006.05.010     Document Type: Article

References (50)

1. Babich H., and Borenfreund E. In vitro cytotoxicity of organic pollutants to bluegill sunfish (BF-2) cells. Environ. Res. 42 (1987) 229-237
2. Babin M.M., Garcia P., Fernandez C., Alonso C., Carbonell G., and Tarazona J.V. Toxicological characterisation of sludge from sewage treatment plants using toxicity identification evaluation protocols based on in vitro toxicity tests. Toxicol. In Vitr. 15 (2001) 519-524
3. Borenfreund E., Babich H., and Martin-Alguacil N. Coparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT test. Toxicol. In Vitr. 2 (1988) 1-6
4. Borenfreund E., and Puerner J.A. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24 (1985) 119-124
5. Brooks B.W., Foran C.M., Richards S.M., Weston J., Turner P.K., Stanley J.K., Solomon K.R., Slattery M., and La Point T.W. Aquatic ecotoxicology of fluoxetine. Toxicol. Lett. 142 (2003) 169-183
6. Brüschweiler B.J., Würgler F.E., and Fent K. Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poeciliopsis lucida). Aquat. Toxicol. 32 (1995) 143-160
7. Calamari D., Zuccato E., Castiglioni S., Bagnati R., and Fanelli R. Strategic survey of therapeutic drugs in the rivers Po and Lambro in northern Italy. Environ. Sci. Technol. 37 (2003) 1241-1248
8. Calleja M.C., Persoone G., and Geladi P. Comparative acute toxicity of the first 50 multicenter evaluation of in-vitro cytotoxicity chemicals to aquatic non-vertebrates. Arch. Environ. Contam. Toxol. 26 (1994) 69-78
9. Castano A., and Gomez-Lechon M.J. Comparison of basal cytotoxicity data between mammalian and fish cell lines: a literature survey. Toxicol. In Vitr. 19 (2005) 695-705
10. Castano A., Cantarino M.J., Castillo P., and Tarazona J.V. Correlations between the RTG-2 cytotoxicity test EC50 and in vivo LC50 rainbow trout bioassay. Chemosphere 32 (1996) 2141-2157
11. Castano A., Vega M., Blazquez T., and Tarazona J.V. Biological alternatives to chemical identification for the ecotoxicological assessment of industrial effluents: the RTG-2 in vitro cytotoxicity test. Environ. Toxicol. Chem. 13 (1994) 1607-1611
12. Clemedson C., Barile F., Ekwall B., Gómez-Lechón M.J., Hall T., Imai K., Logemann M., Monaco F., Ohno T., Segner H., Sjostrom M., Valentino M., Walum E., Wang X., and Ekwall B. MEIC Evaluation of acute systemic toxicity. Part III. In vitro results from 16 additional methods used to test the first 30 reference chemicals and comparative cytotoxicity analysis. ATLA 26 (1998) 93-129
13. Clemedson C., Andersson M., Aoki Y., Barile F.A., Bassi A.M., Calleja M.C., Castaño A., Clothier R.H., Dierickx P., Ekwall B., Ferro M., Fiskesjö G., Garza-Ocañas L., Gómez-Lechón M.J., Gülden M., and Hall T. MEIC Evaluation of acute systemic toxicity. Part IV. In vitro results from 67 toxicity assays used to test reference chemicals 31-50 and a comparative cytotoxicity analysis. ATLA 26 (1998) 131-183
14. Cleuvers M. Mixture toxicity of the anti-inflammatory drugs diclofenac, ibuprofen, naproxen, and acetylsalicylic acid. Ecotoxicol. Environ. Saf. 59 (2004) 309-315
15. Cleuvers M. Aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects. Toxicol Lett. 142 (2003) 185-194
16. Daughton C.G., and Ternes T.A. Pharmaceuticals and personal care products in the environment: agents of subtle change?. Environ. Health Perspect. 107 Suppl. 6 (1999) 907-938
17. Desbrow C., Routledge E.J., Brighty G.C., Sumpter J.P., and Waldock M. Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening. Environ. Sci. Technol. 32 (1998) 1549-1558
18. EMEA (2006). Guideline on the environmental risk assessment of medicinal products for human use, EMEA/CHMP/4447/00.The European Agency for the evaluation of medicinal products (EMEA), London.
19. EUROPEAN UNION (2001) WHITE PAPER; Strategy for a future chemicals policy. COM (2001) 88 final, Brussels.
20. FDA-CDER, 1998. Guidance for industry-Environmental assessment of human drugs and biologics applications, Revision 1. FDA Center for Drug Evaluation and Research, Rockville.
21. Fent, K., Escher, C., Caminada, D. Estrogenic activity of pharmaceuticals and pharmaceutical mixtures in a yeast reporter gene system. Reprod Toxicol., in press.
22. Fent K., Weston A.A., and Caminada D. Ecotoxicology of human pharmaceuticals. Aquat. Toxicol. 76 (2006) 122-159
23. Fent K. Fish cell lines as versatile tools in ecotoxicology: assessment of cytotoxicity, cytochrome P4501A induction potential and estrogenic activity of chemicals and environmental samples. Toxicol. In Vitr. 15 (2001) 477-488
24. Fent K., and Hunn J. Cytotoxicity of organic environmental chemicals to fish liver cells (PLHC-1). Mar. Environ. Res. 42 (1996) 377-382
25. Ferrari B., Mons R., Vollat B., Fraysse B., Paxeus N., Lo Giudice R., Pollio A., and Garric J. Environmental risk assessment of six human pharmaceuticals: are the current environmental risk assessment procedures sufficient for the protection of the aquatic environment?. Environ. Toxicol Chem. 23 (2004) 1344-1354
26. Forth W., Henschler D., Rummel W., Förstermann U., and Starke K. Allgemeine und Spezielle Pharmakologie und Toxikologie (2001), Urban & Fischer Verlag München, Jena
27. Halling-Sorensen B., Nors Nielsen S., Lanzky P.F., Ingerslev F., Holten Lutzhoft H.C., and Jorgensen S.E. Occurrence, fate and effects of pharmaceutical substances in the environment-a review. Chemosphere 36 (1998) 357-393
28. Hernando M.D., Petrovic M., Fernandez-Alba A.R., and Barcelo D. Analysis by liquid chromatography-electrospray ionization tandem mass spectrometry and acute toxicity evaluation for beta-blockers and lipid-regulating agents in wastewater samples. J. Chromatogr. A 1046 (2004) 133-140
29. Hollert H., Durr M., Erdinger L., and Braunbeck T. Cytotoxicity of settling particulate matter and sediments of the Neckar river (Germany) during a winter flood. Environ. Toxicol. Chem. 19 (2000) 528-534
30. Hutchinson T.H., Barrett S., Buzby M., Constable D., Hartmann A., Hayes E., Huggett D., Laenge R., Lillicrap A.D., Straub J.O., and Thompson R.S. A strategy to reduce the numbers of fish used in acute ecotoxicity testing of pharmaceuticals. Environ. Toxicol. Chem. 22 (2003) 3031-3036
31. Knie J., Halke A., Juhnke I., and Schiller W. Results of studies on chemical substances with four biotests. (Ergebnisse der Untersuchungen von chemischen Stoffen mit vier Biotests). Dtsch Gewaesserkd Mitt. 27 (1983) 77-79
32. Kolpin D.W., Furlong E.T., Meyer M.T., Thurman E.M., Zaugg S.D., Barber L.B., and Buxton H.T. Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance. Environ. Sci. Technol. 36 (2002) 1202-1211
33. Krone P.H., Blechinger S.R., Evans T.G., Ryan J.A., Noonan E.J., and Hightower L.E. Use of fish liver PLHC-1 cells and zebrafish embryos in cytotoxicity assays. Methods 35 (2005) 176-187
34. Kümmerer K. Pharmaceuticals in the environment. second ed. (2004), Springer-Verlag
35. Kunz P.Y., Galicia H.F., and Fent K. Comparison of in vitro and in vivo estrogenic activity of UV filters in fish. Toxicol. Sci. 90 (2006) 349-361
36. Laville N., Ait-Aissa S., Gomez E., Casellas C., and Porcher J.M. Effects of human pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes. Toxicology 196 (2004) 41-55
37. Li H., and Zhang S. In vitro cytotoxicity of the organophosphorus insecticide methylparathion to FG-9307, the gill cell line of flounder (Paralichthys olivaceus). Cell. Biol. Toxicol. 18 (2002) 235-241
38. Lilius H., Isomaa B., and Holmstrom T. A comparison of the toxicity of 50 reference chemicals to freshly isolated rainbow trout hepatocytes and Daphnia magna. Aquat. Toxicol. 30 (1994) 47-60
39. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 (1983) 55-63
40. Ni Shuilleabhain S., Mothersill C., Sheehan D., O'Brien N.M., O'Halloran J., Van Pelt F.N.A.M., and Davoren M. In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines. Toxicol. in Vitr. 18 (2004) 365-376
41. Oaks J.L., Gilbert M., Virani M.Z., Watson R.T., Meteyer C.U., Rideout B.A., Shivaprasad H.L., Ahmed S., Chaudhry M.J.I., Arshad M., Mahmood S., Ali A., and Khan A.A. Diclofenac residues as the cause of vulture population decline in Pakistan. Nature 427 (2004) 630-633
42. Pomati F., Castiglioni S., Zuccato E., Fanelli R., Vigetti D., Rossetti C., and Calamari D. Effects of a complex mixture of therapeutic drugs at environmental levels on human embryonic cells. Environ. Sci. Technol. 40 (2006) 2442-2447
43. Routledge E.J., and Sumpter J.P. Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen. Environ. Toxicol. Chem. 15 (1996) 241-248
44. Ryan J.A., and Hightower L.E. Evaluation of heavy metal ion toxicity in fish cells using a combined stress and cytotoxicity assay. Environ. Toxicol. Chem. 13 (1994) 1231-1240
45. Saito H., Sudo M., Shigeoka T., and Yamauchi F. In vitro cytotoxicity of chlorophenols to goldfish GF-Scale (GFS) cells an quantitiaive structure-activitiy relationships. Environ. Toxicol. Chem. 10 (1991) 235-241
46. Schwaiger J., Ferling H., Mallow U., Wintermayr H., and Negele R.D. Toxic effects of the non-steroidal anti-inflammatory drug diclofenac. Part I. histopathological alterations and bioaccumulation in rainbow trout. Aquat. Toxicol. 68 (2004) 141-150
47. Segner H. In: Braunbeck T., Hinton D.E., and Streit B. (Eds). Fish cell lines as a tool in aquatic toxicology (1998), Birkhäuser Verlag, Basel 1-38
48. Segner H., and Lenz D. Cytotoxicity assays with the rainbow trout R1 cell line. Toxicol. in Vitr. 7 (1993) 537-540
49. van Wezel A.P., and Opperhuizen A. Narcosis due to environmental pollutants in aquatic organisms: residue-based toxicity, mechanisms, and membrane burdens. Crit. Rev. Toxicol. 25 (1995) 255-279
50. Wiegel S., Aulinger A., Brockmeyer R., Harms H., Loffler J., Reincke H., Schmidt R., Stachel B., Von Tumpling W., and Wanke A. Pharmaceuticals in the river Elbe and its tributaries. Chemosphere 57 (2004) 107-126