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Volumn 48, Issue 2, 2006, Pages 281-291

Expression and purification of His-tagged HPV16 E7 protein active in pRb binding

Author keywords

BIAcore; E7; ELISA; Hexahistidine tag; Human papillomavirus; pRb; ProteoExpert; SPR

Indexed keywords

HISTIDINE; ONCOGENE PROTEIN E7, HUMAN PAPILLOMAVIRUS TYPE 16; ONCOPROTEIN; RECOMBINANT PROTEIN; RETINOBLASTOMA PROTEIN;

EID: 33746218398     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.04.017     Document Type: Article
Times cited : (15)

References (59)
  • 1
    • 0024472997 scopus 로고
    • Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers
    • zur Hausen H. Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Res. 49 (1989) 4677-4681
    • (1989) Cancer Res. , vol.49 , pp. 4677-4681
    • zur Hausen, H.1
  • 2
    • 12344253083 scopus 로고    scopus 로고
    • The role of human papilloma virus in the molecular biology of cervical carcinogenesis
    • Motoyama S., Ladines-Llave C.A., Luis Villanueva S., and Maruo T. The role of human papilloma virus in the molecular biology of cervical carcinogenesis. Kobe J. Med. Sci. 50 (2004) 9-19
    • (2004) Kobe J. Med. Sci. , vol.50 , pp. 9-19
    • Motoyama, S.1    Ladines-Llave, C.A.2    Luis Villanueva, S.3    Maruo, T.4
  • 3
    • 0034600355 scopus 로고    scopus 로고
    • Papillomaviruses causing cancer: evasion from host-cell control in early events in carcinogenesis
    • zur Hausen H. Papillomaviruses causing cancer: evasion from host-cell control in early events in carcinogenesis. J. Natl. Cancer Inst. 92 (2000) 690-698
    • (2000) J. Natl. Cancer Inst. , vol.92 , pp. 690-698
    • zur Hausen, H.1
  • 4
    • 0024804363 scopus 로고
    • HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes
    • Hawley-Nelson P., Vousden K.H., Hubbert N.L., Lowy D.R., and Schiller J.T. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO J. 8 (1989) 3905-3910
    • (1989) EMBO J. , vol.8 , pp. 3905-3910
    • Hawley-Nelson, P.1    Vousden, K.H.2    Hubbert, N.L.3    Lowy, D.R.4    Schiller, J.T.5
  • 6
    • 0037330709 scopus 로고    scopus 로고
    • Mechanisms by which DNA tumor virus oncoproteins target the Rb family of pocket proteins
    • Helt A.M., and Galloway D.A. Mechanisms by which DNA tumor virus oncoproteins target the Rb family of pocket proteins. Carcinogenesis 24 (2003) 159-169
    • (2003) Carcinogenesis , vol.24 , pp. 159-169
    • Helt, A.M.1    Galloway, D.A.2
  • 7
    • 0024809865 scopus 로고
    • Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product
    • Munger K., Werness B.A., Dyson N., Phelps W.C., Harlow E., and Howley P.M. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J. 8 (1989) 4099-4105
    • (1989) EMBO J. , vol.8 , pp. 4099-4105
    • Munger, K.1    Werness, B.A.2    Dyson, N.3    Phelps, W.C.4    Harlow, E.5    Howley, P.M.6
  • 8
    • 0024535228 scopus 로고
    • The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product
    • Dyson N., Howley P.M., Munger K., and Harlow E. The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243 (1989) 934-937
    • (1989) Science , vol.243 , pp. 934-937
    • Dyson, N.1    Howley, P.M.2    Munger, K.3    Harlow, E.4
  • 9
    • 0030938421 scopus 로고    scopus 로고
    • Analysis of the interaction between human papillomavirus type 16 E7 and the TATA-binding protein, TBP
    • Phillips A.C., and Vousden K.H. Analysis of the interaction between human papillomavirus type 16 E7 and the TATA-binding protein, TBP. J. Gen. Virol. 78 Pt 4 (1997) 905-909
    • (1997) J. Gen. Virol. , vol.78 , Issue.PART 4 , pp. 905-909
    • Phillips, A.C.1    Vousden, K.H.2
  • 11
    • 0032551661 scopus 로고    scopus 로고
    • A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein
    • Schilling B., De-Medina T., Syken J., Vidal M., and Munger K. A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein. Virology 247 (1998) 74-85
    • (1998) Virology , vol.247 , pp. 74-85
    • Schilling, B.1    De-Medina, T.2    Syken, J.3    Vidal, M.4    Munger, K.5
  • 13
    • 0036132647 scopus 로고    scopus 로고
    • Novel function of the cyclin A binding site of E2F in regulating p53-induced apoptosis in response to DNA damage
    • Hsieh J.K., Yap D., O'Connor D.J., Fogal V., Fallis L., Chan F., Zhong S., and Lu X. Novel function of the cyclin A binding site of E2F in regulating p53-induced apoptosis in response to DNA damage. Mol. Cell Biol. 22 (2002) 78-93
    • (2002) Mol. Cell Biol. , vol.22 , pp. 78-93
    • Hsieh, J.K.1    Yap, D.2    O'Connor, D.J.3    Fogal, V.4    Fallis, L.5    Chan, F.6    Zhong, S.7    Lu, X.8
  • 14
    • 0032146274 scopus 로고    scopus 로고
    • The regulation of E2F by pRB-family proteins
    • Dyson N. The regulation of E2F by pRB-family proteins. Genes Dev. 12 (1998) 2245-2262
    • (1998) Genes Dev. , vol.12 , pp. 2245-2262
    • Dyson, N.1
  • 15
    • 0034899522 scopus 로고    scopus 로고
    • Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7
    • Gonzalez S.L., Stremlau M., He X., Basile J.R., and Munger K. Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7. J. Virol. 75 (2001) 7583-7591
    • (2001) J. Virol. , vol.75 , pp. 7583-7591
    • Gonzalez, S.L.1    Stremlau, M.2    He, X.3    Basile, J.R.4    Munger, K.5
  • 16
    • 0035797506 scopus 로고    scopus 로고
    • Both Rb and E7 are regulated by the ubiquitin proteasome pathway in HPV-containing cervical tumor cells
    • Wang J., Sampath A., Raychaudhuri P., and Bagchi S. Both Rb and E7 are regulated by the ubiquitin proteasome pathway in HPV-containing cervical tumor cells. Oncogene 20 (2001) 4740-4749
    • (2001) Oncogene , vol.20 , pp. 4740-4749
    • Wang, J.1    Sampath, A.2    Raychaudhuri, P.3    Bagchi, S.4
  • 17
    • 2942722811 scopus 로고    scopus 로고
    • Molecular mechanisms of E2F-dependent activation and pRB-mediated repression
    • Frolov M.V., and Dyson N.J. Molecular mechanisms of E2F-dependent activation and pRB-mediated repression. J. Cell Sci. 117 (2004) 2173-2181
    • (2004) J. Cell Sci. , vol.117 , pp. 2173-2181
    • Frolov, M.V.1    Dyson, N.J.2
  • 19
    • 0026550131 scopus 로고
    • Efficiency of binding the retinoblastoma protein correlates with the transforming capacity of the E7 oncoproteins of the human papillomaviruses
    • Heck D.V., Yee C.L., Howley P.M., and Munger K. Efficiency of binding the retinoblastoma protein correlates with the transforming capacity of the E7 oncoproteins of the human papillomaviruses. Proc. Natl. Acad. Sci. USA 89 (1992) 4442-4446
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 4442-4446
    • Heck, D.V.1    Yee, C.L.2    Howley, P.M.3    Munger, K.4
  • 20
    • 0025815414 scopus 로고
    • Biochemical and biological differences between E7 oncoproteins of the high- and low-risk human papillomavirus types are determined by amino-terminal sequences
    • Munger K., Yee C.L., Phelps W.C., Pietenpol J.A., Moses H.L., and Howley P.M. Biochemical and biological differences between E7 oncoproteins of the high- and low-risk human papillomavirus types are determined by amino-terminal sequences. J. Virol. 65 (1991) 3943-3948
    • (1991) J. Virol. , vol.65 , pp. 3943-3948
    • Munger, K.1    Yee, C.L.2    Phelps, W.C.3    Pietenpol, J.A.4    Moses, H.L.5    Howley, P.M.6
  • 22
    • 33746258995 scopus 로고    scopus 로고
    • Structure of the human papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor
    • Liu X., Clements A., Zhao K., and Marmorstein R. Structure of the human papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor. J. Biol. Chem. (2005)
    • (2005) J. Biol. Chem.
    • Liu, X.1    Clements, A.2    Zhao, K.3    Marmorstein, R.4
  • 23
    • 2642601106 scopus 로고    scopus 로고
    • Structure of the retinoblastoma tumour-suppressor pocket domain bound to a peptide from HPV E7
    • Lee J.O., Russo A.A., and Pavletich N.P. Structure of the retinoblastoma tumour-suppressor pocket domain bound to a peptide from HPV E7. Nature 391 (1998) 859-865
    • (1998) Nature , vol.391 , pp. 859-865
    • Lee, J.O.1    Russo, A.A.2    Pavletich, N.P.3
  • 24
    • 0024512974 scopus 로고
    • Papillomavirus polypeptides E6 and E7 are zinc-binding proteins
    • Barbosa M.S., Lowy D.R., and Schiller J.T. Papillomavirus polypeptides E6 and E7 are zinc-binding proteins. J. Virol. 63 (1989) 1404-1407
    • (1989) J. Virol. , vol.63 , pp. 1404-1407
    • Barbosa, M.S.1    Lowy, D.R.2    Schiller, J.T.3
  • 25
    • 0026597152 scopus 로고
    • Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein
    • Phelps W.C., Munger K., Yee C.L., Barnes J.A., and Howley P.M. Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein. J. Virol. 66 (1992) 2418-2427
    • (1992) J. Virol. , vol.66 , pp. 2418-2427
    • Phelps, W.C.1    Munger, K.2    Yee, C.L.3    Barnes, J.A.4    Howley, P.M.5
  • 26
    • 0025022381 scopus 로고
    • The region of the HPV E7 oncoprotein homologous to adenovirus E1a and Sv40 large T antigen contains separate domains for Rb binding and casein kinase II phosphorylation
    • Barbosa M.S., Edmonds C., Fisher C., Schiller J.T., Lowy D.R., and Vousden K.H. The region of the HPV E7 oncoprotein homologous to adenovirus E1a and Sv40 large T antigen contains separate domains for Rb binding and casein kinase II phosphorylation. EMBO J. 9 (1990) 153-160
    • (1990) EMBO J. , vol.9 , pp. 153-160
    • Barbosa, M.S.1    Edmonds, C.2    Fisher, C.3    Schiller, J.T.4    Lowy, D.R.5    Vousden, K.H.6
  • 27
    • 0023667757 scopus 로고
    • Multiple functional domains in the adenovirus E1A gene
    • Moran E., and Mathews M.B. Multiple functional domains in the adenovirus E1A gene. Cell 48 (1987) 177-178
    • (1987) Cell , vol.48 , pp. 177-178
    • Moran, E.1    Mathews, M.B.2
  • 28
    • 0028793343 scopus 로고
    • Dimerization of the human papillomavirus E7 oncoprotein in vivo
    • Clemens K.E., Brent R., Gyuris J., and Munger K. Dimerization of the human papillomavirus E7 oncoprotein in vivo. Virology 214 (1995) 289-293
    • (1995) Virology , vol.214 , pp. 289-293
    • Clemens, K.E.1    Brent, R.2    Gyuris, J.3    Munger, K.4
  • 30
    • 0028231049 scopus 로고
    • Identification of a novel retinoblastoma gene product binding site on human papillomavirus type 16 E7 protein
    • Patrick D.R., Oliff A., and Heimbrook D.C. Identification of a novel retinoblastoma gene product binding site on human papillomavirus type 16 E7 protein. J. Biol. Chem. 269 (1994) 6842-6850
    • (1994) J. Biol. Chem. , vol.269 , pp. 6842-6850
    • Patrick, D.R.1    Oliff, A.2    Heimbrook, D.C.3
  • 32
    • 0023201676 scopus 로고
    • The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoprotein
    • Smotkin D., and Wettstein F.O. The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoprotein. J. Virol. 61 (1987) 1686-1689
    • (1987) J. Virol. , vol.61 , pp. 1686-1689
    • Smotkin, D.1    Wettstein, F.O.2
  • 33
    • 0024748979 scopus 로고
    • The E7 protein of human papillomavirus type 16 is phosphorylated by casein kinase II
    • Firzlaff J.M., Galloway D.A., Eisenman R.N., and Luscher B. The E7 protein of human papillomavirus type 16 is phosphorylated by casein kinase II. New Biol. 1 (1989) 44-53
    • (1989) New Biol. , vol.1 , pp. 44-53
    • Firzlaff, J.M.1    Galloway, D.A.2    Eisenman, R.N.3    Luscher, B.4
  • 34
    • 0032995535 scopus 로고    scopus 로고
    • Expression, purification and immunological characterization of the transforming protein E7, from cervical cancer-associated human papillomavirus type 16
    • Fernando G.J., Murray B., Zhou J., and Frazer I.H. Expression, purification and immunological characterization of the transforming protein E7, from cervical cancer-associated human papillomavirus type 16. Clin. Exp. Immunol. 115 (1999) 397-403
    • (1999) Clin. Exp. Immunol. , vol.115 , pp. 397-403
    • Fernando, G.J.1    Murray, B.2    Zhou, J.3    Frazer, I.H.4
  • 35
    • 0025801048 scopus 로고
    • Expression of human papillomavirus proteins in yeast Saccharomyces cerevisiae
    • Carter J.J., Yaegashi N., Jenison S.A., and Galloway D.A. Expression of human papillomavirus proteins in yeast Saccharomyces cerevisiae. Virology 182 (1991) 513-521
    • (1991) Virology , vol.182 , pp. 513-521
    • Carter, J.J.1    Yaegashi, N.2    Jenison, S.A.3    Galloway, D.A.4
  • 36
    • 0029134617 scopus 로고
    • Sequential activation of cyclin E and cyclin A gene expression by human papillomavirus type 16 E7 through sequences necessary for transformation
    • Zerfass K., Schulze A., Spitkovsky D., Friedman V., Henglein B., and Jansen-Durr P. Sequential activation of cyclin E and cyclin A gene expression by human papillomavirus type 16 E7 through sequences necessary for transformation. J. Virol. 69 (1995) 6389-6399
    • (1995) J. Virol. , vol.69 , pp. 6389-6399
    • Zerfass, K.1    Schulze, A.2    Spitkovsky, D.3    Friedman, V.4    Henglein, B.5    Jansen-Durr, P.6
  • 37
    • 0028144175 scopus 로고
    • Functional oligomerization of purified human papillomavirus types 16 and 6b E7 proteins expressed in Escherichia coli
    • Chinami M., Sasaki S., Hachiya N., Yuge K., Ohsugi T., Maeda H., and Shingu M. Functional oligomerization of purified human papillomavirus types 16 and 6b E7 proteins expressed in Escherichia coli. J. Gen. Virol. 75 Pt 2 (1994) 277-281
    • (1994) J. Gen. Virol. , vol.75 , Issue.PART 2 , pp. 277-281
    • Chinami, M.1    Sasaki, S.2    Hachiya, N.3    Yuge, K.4    Ohsugi, T.5    Maeda, H.6    Shingu, M.7
  • 38
    • 0025251612 scopus 로고
    • Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA synthesis and for trans activation
    • Rawls J.A., Pusztai R., and Green M. Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA synthesis and for trans activation. J. Virol. 64 (1990) 121-6129
    • (1990) J. Virol. , vol.64 , pp. 121-6129
    • Rawls, J.A.1    Pusztai, R.2    Green, M.3
  • 40
    • 0030600382 scopus 로고    scopus 로고
    • Bacterial expression and immunological detection of human papillomavirus type 16 E7 protein
    • Moro A., and Hernandez P. Bacterial expression and immunological detection of human papillomavirus type 16 E7 protein. Biochem. Biophys. Res. Commun. 226 (1996) 895-899
    • (1996) Biochem. Biophys. Res. Commun. , vol.226 , pp. 895-899
    • Moro, A.1    Hernandez, P.2
  • 41
    • 0031194898 scopus 로고    scopus 로고
    • A general purification protocol for E7 proteins from "high- and low-risk" human papillomavirus types expressed in the yeast Schizosaccharomyces pombe
    • Braspenning J., Manetti R., Zumbach K., Meschede W., Gissmann L., and Tommasino M. A general purification protocol for E7 proteins from "high- and low-risk" human papillomavirus types expressed in the yeast Schizosaccharomyces pombe. Protein Expr. Purif. 10 (1997) 192-201
    • (1997) Protein Expr. Purif. , vol.10 , pp. 192-201
    • Braspenning, J.1    Manetti, R.2    Zumbach, K.3    Meschede, W.4    Gissmann, L.5    Tommasino, M.6
  • 42
    • 0027281318 scopus 로고
    • Expression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients
    • Stacey S.N., Ghosh A., Bartholomew J.S., Tindle R.W., Stern P.L., Mackett M., and Arrand J.R. Expression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients. J. Med. Virol. 40 (1993) 14-21
    • (1993) J. Med. Virol. , vol.40 , pp. 14-21
    • Stacey, S.N.1    Ghosh, A.2    Bartholomew, J.S.3    Tindle, R.W.4    Stern, P.L.5    Mackett, M.6    Arrand, J.R.7
  • 43
    • 0025047290 scopus 로고
    • Synthesis, phosphorylation, and nuclear localization of human papillomavirus E7 protein in Schizosaccharomyces pombe
    • Tommasino M., Contorni M., Scarlato V., Bugnoli M., Maundrell K., and Cavalieri F. Synthesis, phosphorylation, and nuclear localization of human papillomavirus E7 protein in Schizosaccharomyces pombe. Gene 93 (1990) 265-270
    • (1990) Gene , vol.93 , pp. 265-270
    • Tommasino, M.1    Contorni, M.2    Scarlato, V.3    Bugnoli, M.4    Maundrell, K.5    Cavalieri, F.6
  • 44
    • 0027717104 scopus 로고
    • Human papillomavirus type 16 E6, E7 and L1 and type 18 E7 proteins produced by recombinant baculoviruses
    • Park D.S., Selvey L.A., Kelsall S.R., and Frazer I.H. Human papillomavirus type 16 E6, E7 and L1 and type 18 E7 proteins produced by recombinant baculoviruses. J. Virol. Methods 45 (1993) 303-318
    • (1993) J. Virol. Methods , vol.45 , pp. 303-318
    • Park, D.S.1    Selvey, L.A.2    Kelsall, S.R.3    Frazer, I.H.4
  • 46
    • 0027516185 scopus 로고
    • One-step purification of recombinant human papillomavirus type 16 E7 oncoprotein and its binding to the retinoblastoma gene product
    • Kasher M.S., Wakulchik M., Cook J.A., and Smith M.C. One-step purification of recombinant human papillomavirus type 16 E7 oncoprotein and its binding to the retinoblastoma gene product. Biotechniques 14 (1993) 630-641
    • (1993) Biotechniques , vol.14 , pp. 630-641
    • Kasher, M.S.1    Wakulchik, M.2    Cook, J.A.3    Smith, M.C.4
  • 47
    • 0026134876 scopus 로고
    • Refolding and purification of human papillomavirus type 16 E7-lacZ fusion protein expressed in Escherichia coli
    • Chinami M., Yuge K., Kawano K., and Shingu M. Refolding and purification of human papillomavirus type 16 E7-lacZ fusion protein expressed in Escherichia coli. Protein Expr. Purif. 2 (1991) 175-178
    • (1991) Protein Expr. Purif. , vol.2 , pp. 175-178
    • Chinami, M.1    Yuge, K.2    Kawano, K.3    Shingu, M.4
  • 48
    • 0037143577 scopus 로고    scopus 로고
    • High-risk (HPV16) human papillomavirus E7 oncoprotein is highly stable and extended, with conformational transitions that could explain its multiple cellular binding partners
    • Alonso L.G., Garcia-Alai M.M., Nadra A.D., Lapena A.N., Almeida F.L., Gualfetti P., and Prat-Gay G.D. High-risk (HPV16) human papillomavirus E7 oncoprotein is highly stable and extended, with conformational transitions that could explain its multiple cellular binding partners. Biochemistry 41 (2002) 10510-10518
    • (2002) Biochemistry , vol.41 , pp. 10510-10518
    • Alonso, L.G.1    Garcia-Alai, M.M.2    Nadra, A.D.3    Lapena, A.N.4    Almeida, F.L.5    Gualfetti, P.6    Prat-Gay, G.D.7
  • 49
    • 0024522254 scopus 로고
    • Human papillomavirus type 16 E7 protein expressed in Escherichia coli and monkey COS-1 cells: immunofluorescence detection of the nuclear E7 protein
    • Sato H., Watanabe S., Furuno A., and Yoshiike K. Human papillomavirus type 16 E7 protein expressed in Escherichia coli and monkey COS-1 cells: immunofluorescence detection of the nuclear E7 protein. Virology 170 (1989) 311-315
    • (1989) Virology , vol.170 , pp. 311-315
    • Sato, H.1    Watanabe, S.2    Furuno, A.3    Yoshiike, K.4
  • 50
  • 51
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 53
    • 0034719111 scopus 로고    scopus 로고
    • Oligomerization properties of the viral oncoproteins adenovirus E1A and human papillomavirus E7 and their complexes with the retinoblastoma protein
    • Clements A., Johnston K., Mazzarelli J.M., Ricciardi R.P., and Marmorstein R. Oligomerization properties of the viral oncoproteins adenovirus E1A and human papillomavirus E7 and their complexes with the retinoblastoma protein. Biochemistry 39 (2000) 16033-16045
    • (2000) Biochemistry , vol.39 , pp. 16033-16045
    • Clements, A.1    Johnston, K.2    Mazzarelli, J.M.3    Ricciardi, R.P.4    Marmorstein, R.5
  • 54
    • 0026002137 scopus 로고
    • Purification and characterization of human papillomavirus type 16 E7 protein with preferential binding capacity to the underphosphorylated form of retinoblastoma gene product
    • Imai Y., Matsushima Y., Sugimura T., and Terada M. Purification and characterization of human papillomavirus type 16 E7 protein with preferential binding capacity to the underphosphorylated form of retinoblastoma gene product. J. Virol. 65 (1991) 4966-4972
    • (1991) J. Virol. , vol.65 , pp. 4966-4972
    • Imai, Y.1    Matsushima, Y.2    Sugimura, T.3    Terada, M.4
  • 55
    • 0034044451 scopus 로고    scopus 로고
    • Analysis of relative binding affinity of E7-pRB of human papillomavirus 16 clinical variants using the yeast two-hybrid system
    • Choo K.B., Wang T.S., and Huang C.J. Analysis of relative binding affinity of E7-pRB of human papillomavirus 16 clinical variants using the yeast two-hybrid system. J. Med. Virol. 61 (2000) 298-302
    • (2000) J. Med. Virol. , vol.61 , pp. 298-302
    • Choo, K.B.1    Wang, T.S.2    Huang, C.J.3
  • 56
    • 0034845467 scopus 로고    scopus 로고
    • Determination of the binding affinity of different human papillomavirus E7 proteins for the tumour suppressor pRb by a plate-binding assay
    • Dong W.L., Caldeira S., Sehr P., Pawlita M., and Tommasino M. Determination of the binding affinity of different human papillomavirus E7 proteins for the tumour suppressor pRb by a plate-binding assay. J. Virol. Methods 98 (2001) 91-98
    • (2001) J. Virol. Methods , vol.98 , pp. 91-98
    • Dong, W.L.1    Caldeira, S.2    Sehr, P.3    Pawlita, M.4    Tommasino, M.5
  • 58
    • 0035289707 scopus 로고    scopus 로고
    • Induction of apoptosis in cervical carcinoma cells by peptide aptamers that bind to the HPV-16 E7 oncoprotein
    • Nauenburg S., Zwerschke W., and Jansen-Durr P. Induction of apoptosis in cervical carcinoma cells by peptide aptamers that bind to the HPV-16 E7 oncoprotein. FASEB J. 15 (2001) 592-594
    • (2001) FASEB J. , vol.15 , pp. 592-594
    • Nauenburg, S.1    Zwerschke, W.2    Jansen-Durr, P.3
  • 59
    • 23244458064 scopus 로고    scopus 로고
    • Intracellular anti-E7 human antibodies in single-chain format inhibit proliferation of HPV16-positive cervical carcinoma cells
    • Accardi L., Dona M.G., Di Bonito P., and Giorgi C. Intracellular anti-E7 human antibodies in single-chain format inhibit proliferation of HPV16-positive cervical carcinoma cells. Int. J. Cancer (2005)
    • (2005) Int. J. Cancer
    • Accardi, L.1    Dona, M.G.2    Di Bonito, P.3    Giorgi, C.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.