A novel real-time PCR assay for the speciation of medically important ticks

Vector-Borne and Zoonotic Diseases

Volumn 6, Issue 2, 2006, Pages 152-160

  Shone, Scott M ^a,b   Dillon, Harry J ^b   Hom, Sherman S ^b   Delgado, Nelson ^b  

^a NATL CENTER FOR INFECTIOUS DISEASES   (United States)

^b NEW JERSEY DEPARTMENT OF HEALTH AND SENIOR SERVICES   (United States)

Author keywords

Amblyomma; Dermacentor; Ixodes; Vector

Indexed keywords

CELL NUCLEUS DNA; INTERNAL TRANSCRIBED SPACER; OLIGONUCLEOTIDE; RIBOSOME DNA;

AMBLYOMMA; ARTICLE; CONTROLLED STUDY; DATA BASE; DERMACENTOR; DNA SEQUENCE; IXODES SCAPULARIS; MATHEMATICAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS; SPECIES DIFFERENTIATION; SPECIES IDENTIFICATION; TICK; UNITED STATES;

ANIMALS; ARACHNID VECTORS; BASE SEQUENCE; DERMACENTOR; DNA, INTERGENIC; DNA, RIBOSOMAL SPACER; IXODES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PHYLOGENY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TICKS;

ACARI; AMBLYOMMA AMERICANUM; DERMACENTOR VARIABILIS; IXODES SCAPULARIS; IXODIDA;

EID: 33746119811     PISSN: 15303667     EISSN: None     Source Type: Journal    
DOI: 10.1089/vbz.2006.6.152     Document Type: Article

References (32)

1. Adelson, ME, Rao, RV, Tilton, RC, et al. Prevalence of Borrelia burgdorferi, Bartonella spp., Babesia microti, and Anaplasma phagocytophila in Ixodes scapularis ticks collected in Northern New Jersey. J Clin Microbiol 2004; 6:2799-2801.
2. Altschul, SF, Madden, TL, Schaffer, AA, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 17:3389-3402.
3. Anderson, JM, Ammerman, NC, Norris, DE. Molecular differentiation of metastriate tick immatures. Vector-Borne Zoonotic Dis 2004; 4:334-342.
4. Apperson, CS, Harrison, BA, Unnasch, TR, et al. Host-feeding habits of Culex and other mosquitoes (Diptera: Culicidae) in the Borough of Queens in New York City, with characters and techniques for identification of Culex mosquitoes. J Med Entomol 2002; 5:777-785.
5. Bakken, JS, Dumler, JS. Proper nomenclature for the human granulocytic ehrlichiosis agent. Emerg Infect Dis 2001; 3:486.
6. Black IV, WC, Piesman, J. Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc Natl Acad Sci USA 1994; 21:10034-10038.
7. Caporale, DA, Rich, SM, Spielman, A, et al. Discriminating between Ixodes ticks by means of mitochondrial DNA sequences. Mol Phylogenet Evol 1995; 4:361-365.
8. Childs, JE, Paddock, CD. The ascendancy of Amblyomma americanum as a vector of pathogens affecting humans in the United States. Annu Rev Entomol 2003; 48:307-337.
9. Dobson, SJ, Barker, SC. Phylogeny of the hard ticks (Ixodidae) inferred from 18S rRNA indicates that the genus Aponomma is paraphyletic. Mol Phylogenet Evol 1999; 2:288-295.
10. Dumler, JS, Bakken, JS. Human ehrlichioses: newly recognized infections transmitted by ticks. Annu Rev Med 1998; 49:201-213.
11. Ebel, GD, Campbell, EN, Goethert, HK, et al. Enzootic transmission of deer tick virus in New England and Wisconsin sites. Am J Trop Med Hyg 2000; 1-2:36-42.
12. Furman, DP, Loomis, EC. The ticks of California (Acari: Ixodida). Bull. Calif. Insect 1984; 25:1-239.
13. Halos, L, Jamal, T, Vial, L, et al. Determination of an efficient and reliable method for DNA extraction from ticks. Vet Res 2004; 6:709-713.
14. Hill, SM, Crampton, JM. DNA-based methods for the identification of insect vectors. Ann Trop Med Parasitol 1994; 3:227-250.
15. Keirans, JE, Litwak, TR. Pictorial key to the adults of hard ticks, family Ixodidae (Ixodida: Ixodoidea), East of the Mississippi River. J Med Entomol 1989; 5:435-448.
16. Kogut, SJ, Thill, CD, Prusinski, MA, et al. Babesia microti, upstate New York. Emerg Infect Dis 2005; 3:476-478.
17. Lane, RS, Lavoie, PE. Lyme borreliosis in California. Acarological, clinical, and epidemiological studies. Ann NY Acad Sci 1988; 539:192-203.
18. Mangold, AJ, Bargues, MD, Mas-Coma, S. 18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae). Parasitol Res 1998a; 1:31-37.
19. Mangold, AJ, Bargues, MD, Mas-Coma, S. Mitochondrial 16S rDNA sequences and phylogenetic relationships of species of Rhipicephalus and other tick genera among Metastriata (Acari: Ixodidae). Parasitol Res 1998b; 6:478-484.
20. Murrell, A, Campbell, NJ, Barker, SC. The value of idiosyncratic markers and changes to conserved tRNA sequences from the mitochondrial genome of hard ticks (Acari: Ixodida: Ixodidae) for phylogenetic inference. Syst Biol 2003; 3:296-310.
21. Norris, DE. Genetic markers for study of the anopheline vectors of human malaria. Int J Parasitol 2002; 13:1607-1615.
22. Norris, DE, Klompen, JS, Keirans, JE, et al. Population genetics of Ixodes scapularis (Acari: Ixodidae) based on mitochondrial 16S and 12S genes. J Med Entomol 1996; 1:78-89.
23. Norris, DE, Klompen, JS, Keirans, JE, et al. Taxonomic status of Ixodes neotomae and I. spinipalpis (Acari: Ixodidae) based on mitochondrial DNA evidence. J Med Entomol 1997; 6:696-703.
24. Parola, P, Raoult, D. Tick-borne bacterial diseases emerging in Europe. Clin Microbiol Infect 2001; 2:80-83.
25. Poucher, KL, Hutcheson, HJ, Keirans, JE, et al. Molecular genetic key for the identification of 17 Ixodes species of the United States (Acari: Ixodidae): a methods model. J Parasitol 1999; 4:623-629.
26. Schulze, TL, Jordan, RA, Hung, RW, et al. Prevalence of Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) in Ixodes scapularis (Acari: Ixodidae) adults in New Jersey, 2000-2001. J Med Entomol 2003; 4:555-558.
27. Schulze, TL, Jordan, RA, Schulze, CJ, et al. Relative encounter frequencies and prevalence of selected Borrelia, Ehrlichia, and Anaplasma infections in Amblyomma americanum and Ixodes scapularis (Acari: Ixodidae) ticks from central New Jersey. J Med Entomol 2005; 3:450-456.
28. Sonenshine, D. Ticks of Virginia (Acari: Metastigmata). Blacksburg: Virginia Polytechnic Institute and State University, 1979.
29. Sonenshine, D. Biology of Ticks, Vol. I. New York: Oxford University Press, 1991.
30. Thompson, JD, Gibson, TJ, Plewniak, F, et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876-4882.
31. Varde, S, Beckley, J, Schwartz, I. Prevalence of tick-borne pathogens in Ixodes scapularis in a rural New Jersey County. Emerg Infect Dis 1998; 1:97-99.
32. Walker, DH. Tick-transmitted infectious diseases in the United States. Annu Rev Public Health 1998; 19:237-269.