Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli

Applied and Environmental Microbiology

Volumn 72, Issue 7, 2006, Pages 4735-4742

  Yamada, Satoshi ^a   Awano, Naoki ^a   Inubushi, Kyoko ^a   Maeda, Eri ^a   Nakamori, Shigeru ^a   Nishino, Kunihiko ^b   Yamaguchi, Akihito ^b   Takagi, Hiroshi ^a,c  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; DRUG THERAPY; ENZYME INHIBITION; GENETIC ENGINEERING; GLUCOSE; PROTEINS;

CYSTEINE DESULFHYDRASE (CD); DRUG TRANSPORTER GENES; GENE ASSAYS; GENE ENCODING;

ESCHERICHIA COLI;

BACTERIAL PROTEIN; BICOZAMYCIN; BREAKPOINT CLUSTER REGION PROTEIN; CARRIER PROTEIN; CYSTATHIONINE GAMMA LYASE; CYSTEINE; GLUCOSE; PROTEIN YDED; PROTEIN YFIK; SERINE ACETYLTRANSFERASE; TETRACYCLINE; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; GENE EXPRESSION; GENETIC ENGINEERING;

ACRD GENE; ACREF GENE; AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BCR GENE; CUSA GENE; DEGRADATION; EMRAB GENE; EMRKY GENE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; GROWTH INHIBITION; NEGATIVE FEEDBACK; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; REPORTER GENE; TNAA GENE; YBJYZ GENE; YOJIH GENE;

ANTI-BACTERIAL AGENTS; BACTERIAL OUTER MEMBRANE PROTEINS; BICYCLO COMPOUNDS, HETEROCYCLIC; CULTURE MEDIA; CYSTEINE; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; TETRACYCLINE;

ESCHERICHIA COLI;

EID: 33746060524     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02507-05     Document Type: Article

References (39)

1. Awano, N., M. Wada, A. Kohdoh, T. Oikawa, H. Takagi, and S. Nakamori. 2003. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl. Microbiol. Biotechnol. 62:239-243.
2. Awano, N., M. Wada, H. Mori, S. Nakamori, and H. Takagi. 2005. Identification and functional analysis of cysteine desulfhydrases in Escherichia coli. Appl. Environ. Microbiol. 71:4149-4152.
3. Bentley, J., L. S. Hyatt, K. Ainley, J. H. Parish, R. B. Herbert, and G. R. White. 1993. Cloning and sequence analysis of an Escherichia coli gene conferring bicyclomycin resistance. Gene 127:117-120.
4. Bolhuis, H., H. W. van Veen, B. Poolman, A. J. Driessen, and W. N. Konings. 1997. Mechanisms of multidrug transporters. FEMS Microbiol. Rev. 21:55-84.
5. Cook, G. M., and R. K. Poole. 2000. Oxidase and periplasmic cytochrome assembly in Escherichia coli K-12: CydDC and CcmAB are not required for haem-membrane association. Microbiology 146:527-536.
6. Daßler, T., T. Maier, C. Winterhalter, and A. Böck. 2000. Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway. Mol. Microbiol. 36:1101-1112.
7. De Felice, M., C. Squires, M. Levinthal, J. Guardiola, A. Lamberti, and M. Iaccarino. 1977. Growth inhibition of Escherichia coli K-12 by L-valine: a consequence of a regulatory pattern. Mol. Gen. Genet. 156:1-7.
8. Delaney, J. M., D. Ang, and C. Georgopoulos. 1992. Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures. J. Bacteriol. 174:1240-1247.
9. Denk, D., and A. Böck. 1987. L-Cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and cysteine excreting mutant. J. Gen. Microbiol. 133:515-525.
10. Dwivedi, C. M., R. C. Ragin, and J. R. Uren. 1982. Cloning, purification, and characterization of β-cystathionase from Escherichia coli. Biochemistry 21:3064-3069.
11. Eisenberg, D., E. Schwarz, M. Komaromy, and R. Wall. 1984. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179:125-142.
12. Franke, I., A. Resch, T. Daßler, T. Maier, and A. Böck. 2003. YfiK from Escherichia coli promotes export of O-acetylserine and cysteine. J. Bacteriol. 185:1161-1166.
13. Gaitonde, M. K. 1967. A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochem. J. 104:627-633.
14. Grass, G., and C. Rensing. 2001. Genes involved in copper homeostasis in Escherichia coli. J. Bacteriol. 183:2145-2147.
15. Harris, C. L. 1981. Cysteine and growth inhibition of Escherichia coli: threonine deaminase as the target enzyme. J. Bacteriol. 145:1031-1035.
16. Harris, C. L., and L. Lui. 1981. Cysteine and growth inhibition of Escherichia coli: depression of the ilvGEDA operon. Biochem. Biophys. Res. Commun. 101:1145-1151.
17. Kari, C., Z. Nagy, P. Kovacs, and F. Hernadi. 1971. Mechanism of the growth inhibitory effect of cysteine on Escherichia coli. J. Gen. Microbiol. 68:349-356.
18. Kawamura-Sato, K., K. Shibayama, T. Horii, Y. Iimuma, Y. Arakawa, and M. Ohta. 1999. Role of multiple efflux pumps in Escherichia coli in indole expulsion. FEMS Microbiol. Lett. 179:345-352.
19. Kredich, N. M. 1983. Regulation of cysteine biosynthesis in Escherichia coli and Salmonella typhimurium, p. 115-132. In K. M. Herrmann and R. L. Sommerville (ed.), Amino acids: biosynthesis and genetic regulation. Addison-Wesley Publishing Company, London, United Kingdom.
20. Lomovskaya, O., and K. Lewis. 1992. Emr, an Escherichia coli locus for multidrug resistance. Proc. Natl. Acad. Sci. USA 89:8938-8942.
21. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
22. Nakamori, S., S. Kobayashi, C. Kobayashi, and H. Takagi. 1998. Overproduction of L-cysteine and L-cystine by Escherichia coli strains with a genetically altered serine acetyltransferase. Appl. Environ. Microbiol. 64:1607-1611.
23. Newton, W. A., and E. F. Snell. 1964. Catalytic properties of tryptophanase, a multifunctional pyridoxal phosphate enzyme. Proc. Natl. Acad. Sci. USA 51:382-389.
24. Newton, W. A., Y. Morino, and E. F. Snell. 1965. Properties of crystalline tryptophanase. J. Biol. Chem. 240:1211-1218.
25. Nishino, K., and A. Yamaguchi. 2001. Analysis of a complete library of putative drug transporter genes in Escherichia coli. J. Bacteriol. 183:5803-5812.
26. Park, S., and J. A. Imlay. 2003. High levels of intracellular cysteine promote oxidative DNA damage by driving the Fenton reaction. J. Bacteriol. 185:1942-1950.
27. Pittman, M. S., H. Corker, G. Wu, M. B. Binet, A. J. G. Moir, and R. K. Poole. 2002. Cysteine is exported from the Escherichia coli cytoplasm by CydDC, an ATP-binding cassette-type transporter required for cytochrome assembly. J. Biol. Chem. 277:49841-49849.
28. Putman, M., H. W. van Veen, and W. N. Konings. 2000. Molecular properties of bacterial multidrug transporters. Microbiol. Mol. Biol. Rev. 64:672-693.
29. Rosenberg, E. Y., D. Ma, and H. Nikaido. 2000. AcrD of Escherichia coli is an aminoglycoside efflux pump. J. Bacteriol. 182:1754-1756.
30. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sivaraman, J., V. Sauvé, R. Larocque, E. A. Stura, J. D. Schrag, M. Cygler, and A. Matte. 2002. Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP. Nat. Struct. Biol. 9:353-358.
32. Soda, K. 1987. Microbial sulfur amino acids: an overview. Methods Enzymol. 143:453-459.
33. Sorensen, M. A., and S. Pedersen. 1991. Cysteine, even in low concentrations, induces transient amino acid starvation in Escherichia coli. J. Bacteriol. 173:5244-5246.
34. Takagi, H., C. Kobayashi, S. Kobayashi, and S. Nakamori. 1999. PCR random mutagenesis into Escherichia coli serine acetyltransferase: isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition. FEBS Lett. 452:323-327.
35. Takagi, H., N. Awano, S. Kobayashi, C. Kobayashi, M. Noji, K. Saito, and S. Nakamori. 1999. Overproduction of L-cysteine and L-cystine by expression of genes for feedback inhibition-insensitive serine acetyltransferase from Arabidopsis thaliana in Escherichia coli. FEMS Microbiol. Lett. 179:453-459.
36. Tsunoda, T., S. Eguchi, and K. Narumi. 1961. On the bioassay of amino acids. II. Determination of arginine, aspartic acid and cysteine. Amino Acids 3:7-13.
37. Vedantam, G., G. G. Guay, N. E. Austria, S. Z. Doktor, and B. P. Nichols. 1998. Characterization of mutations contributing to sulfathiazole resistance in Escherichia coli. Antimicrob. Agents Chemother. 42:88-93.
38. Wrzesinski, J., A. Bakin, K. Nurse, B. G. Lane, and J. Ofengand. 1995. Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli. Biochemistry 34:8904-8913.
39. + antiporter of Escherichia coli encoded by transposon Tn10. The structural resemblance and functional difference in the role of the duplicated sequence motif between hydrophobic segments 2 and 3 and segments 8 and 9. J. Biol. Chem. 268:6496-6504.