Engineering the substrate specificity of porcine kidney D-amino acid oxidase by mutagenesis of the "active-site lid"

Journal of Biochemistry

Volumn 139, Issue 5, 2006, Pages 873-879

  Setoyama, Chiaki ^a   Nishina, Yasuzo ^a   Mizutani, Hisashi ^b   Miyahara, Ikuko ^b   Hirotsu, Ken ^b   Kamiya, Nobuo ^b   Shiga, Kiyoshi ^a   Miura, Retsu ^a  

^a KUMAMOTO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

Author keywords

D amino acid oxidase; D aspartate oxidase; Directed evolution; Mutagenesis; Substrate specificity

Indexed keywords

ASPARTIC ACID; DEXTRO ALANINE; DEXTRO AMINO ACID OXIDASE; DEXTRO ARGININE; DEXTRO SERINE; ENZYME; ENZYME DEXTRO ASPARTATE OXIDASE; HYBRID PROTEIN; MUTANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME SPECIFICITY; HUMAN; HUMAN TISSUE; MUTAGENESIS; NONHUMAN; STEADY STATE; SUBSTITUTION REACTION;

ANIMALS; BINDING SITES; BRAIN; CLONING, MOLECULAR; D-AMINO-ACID OXIDASE; D-ASPARTATE OXIDASE; HUMANS; KIDNEY; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SWINE;

SUIDAE; SUS SCROFA;

EID: 33745738478     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj094     Document Type: Article

References (15)

1. Curti, B., Ronchi, S., and Pilone, M.S. (1992) D- and L-Amino acid oxidases. In Chemistry and Biochemistry of Flavoenzymes, Vol. 3 (Müller, F., ed.) pp. 69-94, CRC Press, Boca Raton
2. Pilone, M.S. (2000) D-Amino acid oxidase: new findings. Cell. Mol. Life Sci. 57, 1732-1747
3. Negri, A., Ceciliani, G., Tedeschi, G., Simonic, T., and Ronchi, S. (1992) The primary structure of the flavoprotein D-aspartate oxidase from beef kidney. J. Biol. Chem. 267, 11865-11871
4. Mizutani, H., Miyahara, I., Hirotsu, K., Nishina, Y., Shiga, K., Setoyama, C., and Miura, R. (1996) Three-dimensional structure of porcine kidney D-amino acid oxidase at 3.0 Å resolution. J. Biochem. 120, 14-17
5. 2. Proc. Natl Acad. Sci. USA 93, 7496-7501
6. Setoyama, C. and Miura, R. (1997) Structural and functional characterization of the human brain D-aspartate oxidase. J. Biochem. 121, 798-803
7. Todone, F., Vanoni, M.A., Mozzarelli, A., Bolognesi, M., Coda, A., Curti, B., and Mattevi, A. (1997) Active site plasticity in D-amino acid oxidase: A crystallographic analysis. Biochemistry 36, 5853-5860
8. Miura, R., Setoyama, C., Nishina, Y., Shiga, K., Mizutani, H., Miyahara, I., and Hirotsu, K. (1997) Structural and mechanistic studies on D-amino acid oxidase-substrate complex: Implications of the crystal structure of enzyme-substrate analog complex. J. Biochem. 122, 825-833
9. Umhau, S., Pollegioni, L., Molla, G., Diederichs, K., Welte, W., Pilone, M.S., and Ghisla, S. (2000) The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation. Proc. Natl. Acad. Sci. USA 97, 12463-12468
10. Pollegioni, L., Diederichs, K., Molla, G., Umhau, S., Welte, W., Ghisla, S., and Pilone, M.S. (2002) Yeast D-amino acid oxidase: structural basis of its catalytic properties. J. Mol. Biol. 324, 535-546
11. Sacchi, S., Lorenzi, S., Molla, G., Pilone, M.S., Rossetti, C., and Pollegioni, L. (2002) Engineering the substrate specificity of D-amino acid oxidase. J. Biol. Chem. 277, 27510-27516
12. Sacchi, S., Rosini, E., Molla, G., Pilone, M.S., and Pollegioni, L. (2004) Modulating D-amino acid oxidase substrate specificity: production of an enzyme for analytical determination of all D-amino acids by directed evolution. Protein Engin. Design Selection 17, 517-525
13. Setoyama, C., Miura, R., Nishina, Y., Shiga, K., Mizutani, H., Miyahara, I., and Hirotsu, K. (1996) Crystallization of expressed porcine kidney D-amino acid oxidase and preliminary X-ray crystallographic characterization. J. Biochem. 119, 1114-1117
14. Hashimoto-Gotoh, H., Mizuno, T., Ogasawara, Y., and Nakagawa, M. (1995) An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis. Gene 152, 271-275
15. Pollegioni, L., Fukui, K., and Massey, V. (1994) Studies on the kinetic mechanism of pig kidney D-amino acid oxidase by site-directed mutagenesis of Tyrosine 224 and Tyrosine 228. J. Biol. Chem. 269, 31666-31673