Modulating D-amino acid oxidase substrate specificity: Production of an enzyme for analytical determination of all D-amino acids by directed evolution

Protein Engineering, Design and Selection

Volumn 17, Issue 6, 2004, Pages 517-525

  Sacchi, Silvia ^a   Rosini, Elena ^a   Molla, Gianluca ^a   Pilone, Mirella S ^a   Pollegioni, Loredano ^a  

^a UNIVERSITY OF INSUBRIA   (Italy)

Author keywords

Directed evolution; Flavoprotein; Function structure relationships; Substrate recognition

Indexed keywords

AMINO ACIDS; BIOTECHNOLOGY; CATALYSIS; CATALYSTS; ESCHERICHIA COLI; MUTAGENESIS; OXIDATION;

PROTEIN SURFACES; RHODOTORULA GRACILIS (RGDAAO);

ENZYMES;

AMINO ACID OXIDASE; COMPLEMENTARY DNA; DEXTRO ALANINE; DEXTRO AMINO ACID; DEXTRO ARGININE; DEXTRO ASPARTIC ACID; FLAVOPROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; DIRECTED EVOLUTION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; RHODOTORULA; STRUCTURE ACTIVITY RELATION; SUBSTRATE RECOGNITION;

ALANINE; AMINO ACID SUBSTITUTION; AMINO ACIDS; ANTHRANILIC ACIDS; ARGININE; ASPARTIC ACID; BENZOATES; CATALYSIS; D-AMINO-ACID OXIDASE; DIRECTED MOLECULAR EVOLUTION; ENZYME INHIBITORS; ESCHERICHIA COLI; GENE LIBRARY; KINETICS; MODELS, MOLECULAR; MUTATION; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RHODOTORULA; STEREOISOMERISM; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; NEGIBACTERIA; RHODOSPORIDIUM TORULOIDES;

EID: 8444229656     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh064     Document Type: Article

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