Competitive displacement binding assay on rat brain sections and using a β-imager: Application to μ-opioid ligands

Journal of Neuroscience Methods

Volumn 154, Issue 1-2, 2006, Pages 60-67

  Poisnel, Géraldine ^a   Quentin, Thomas ^a   Barré, Louisa ^a   Coquerel, Antoine ^b,c   Debruyne, Danièle ^a,b,c  

^a GIP CYCERON   (France)

^b Drug Dependence Evaluation Centre   (France)

^c CAEN UNIVERSITY HOSPITAL   (France)

Author keywords

Imager; Opioid ligand; Affinity; Autoradiography; Displacement binding; Inhibition constant; Rat brain sections

Indexed keywords

ENKEPHALIN[2 DEXTRO ALANINE 4 METHYLPHENYLALANINE 5 GLYCINE]; MORPHINE; MU OPIATE RECEPTOR; NALOXONE; NALTRINDOLE; REMIFENTANIL; TRITIUM;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BINDING ASSAY; BRAIN REGION; BRAIN TISSUE; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; IC 50; IMAGING; ISOTOPE LABELING; MALE; NONHUMAN; PRIORITY JOURNAL; RADIOACTIVITY; RADIOASSAY; RAT; TIME; VALIDATION PROCESS;

ANALGESICS, OPIOID; ANIMALS; AUTORADIOGRAPHY; BETA RAYS; BINDING, COMPETITIVE; BRAIN; DIAGNOSTIC IMAGING; ENKEPHALIN, ALA(2)-MEPHE(4)-GLY(5)-; HISTOCYTOCHEMISTRY; ISOTOPE LABELING; KINETICS; LIGANDS; MALE; RATS; RATS, WISTAR; RECEPTORS, OPIOID, DELTA; RECEPTORS, OPIOID, KAPPA; RECEPTORS, OPIOID, MU; REPRODUCIBILITY OF RESULTS; TRITIUM;

EID: 33745438690     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.11.012     Document Type: Article

References (31)

1. Ananthan S., Khare N.K., Saini S.K., Seitz L.E., Bartlett J.L., Davis P., et al. Identification of opioid ligands possessing mixed micro agonist/delta antagonist activity among pyridomorphinans derived from naloxone, oxymorphone, and hydromorphone [correction of hydropmorphone]. J Med Chem 47 (2004) 1400-1412
2. Carstairs J.R., Nimmo A.J., and Barnes P.J. Autoradiographic visualization of beta-adrenoceptor subtypes in human lung. Am Rev Respir Dis 132 (1985) 541-547
3. Charpak G., Dominik W., and Zaganidis N. Optical imaging of the spatial distribution of beta-particles emerging from surfaces. Proc Natl Acad Sci USA 86 (1989) 1741-1745
4. Cheng Y., and Prusoff W.H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 percent inhibition (I50) of an enzymatic reaction. Biochem Pharmacol 22 (1973) 3099-3108
5. Clayson J., Jales A., Tyacke R.J., Hudson A.L., Nutt D.J., Lewis J.W., et al. Selective delta-opioid receptor ligands: potential PET ligands based on naltrindole. Bioorg Med Chem Lett 11 (2001) 939-943
6. Coop A., Rothman R.B., Dersch C., Partilla J., Porreca F., Davis P., et al. Delta opioid affinity and selectivity of 4-hydroxy-3-methoxyindolomorphinan analogues related to naltrindole. J Med Chem 42 (1999) 1673-1679
7. Crumeyrolle-Arias M., Jafarian-Tehrani M., Cardona A., Edelman L., Roux P., Laniece P., et al. Radioimagers as an alternative to film autoradiography for in situ quantitative analysis of 125I-ligand receptor binding and pharmacological studies. Histochem J 28 (1996) 801-809
8. 3H]MK 801 binding to mouse brain sections. Eur J Pharmacol 269 (1994) 325-330
9. Fodor M., Slama A., Guillaume V., Videau C., Csaba Z., Oliver C., et al. Distribution and pharmacological characterization of somatostatin receptor binding sites in the sheep brain. J Chem Neuroanat 12 (1997) 175-182
10. Geary W.A., and Wooten G.F. Quantitative film autoradiography of opiate agonist and antagonist binding in rat brain. J Pharmacol Exp Ther 225 (1983) 234-240
11. Herkenham M., and Pert C.B. In vitro autoradiography of opiate receptors in rat brain suggests loci of "opiatergic" pathways. Proc Natl Acad Sci USA 77 (1980) 5532-5536
12. Houdi A.A., Kottayil S., Crooks P.A., and Butterfield D.A. 3-O-Acetylmorphine-6-O-sulfate: a potent, centrally acting morphine derivative. Pharmacol Biochem Behav 53 (1996) 665-671
13. Ilien B., Galzi J.L., Mejean A., Goeldner M., and Hirth C. A mu-opioid receptor-filter assay. Rapid estimation of binding affinity of ligands and reversibility of long-lasting ligand-receptor complexes. Biochem Pharmacol 37 (1988) 3843-3851
14. Knapp R.J., Malatynska E., Collins N., Fang L., Wang J.Y., Hruby V.J., et al. Molecular biology and pharmacology of cloned opioid receptors. FASEB J 9 (1995) 516-525
15. Kobayashi S., Tang R., Shapiro E., and Lepor H. Characterization and localization of prostatic alpha 1 adrenoceptors using radioligand receptor binding on slide-mounted tissue section. J Urol 150 (1993) 2002-2006
16. Langlois X., te Riele P., Wintmolders C., Leysen J.E., and Jurzak M. Use of the beta-imager for rapid ex vivo autoradiography exemplified with central nervous system penetrating neurokinin 3 antagonists. J Pharmacol Exp Ther 299 (2001) 712-717
17. Lewanowitsch T., and Irvine R.J. Naloxone and its quaternary derivative, naloxone methiodide, have differing affinities for mu, delta, and kappa opioid receptors in mouse brain homogenates. Brain Res 964 (2003) 302-305
18. Mansour A., Fox C.A., Akil H., and Watson S.J. Opioid-receptor mRNA expression in the rat CNS: anatomical and functional implications. Trends Neurosci 18 (1995) 22-29
19. Minami M., and Satoh M. Molecular biology of the opioid receptors: structures, functions and distributions. Neurosci Res 23 (1995) 121-145
20. 3H]-CGP 39653 in rat brain. Br J Pharmacol 119 (1996) 819-828
21. Pan L., Xu J., Yu R., Xu M.M., Pan Y.X., and Pasternak G.W. Identification and characterization of six new alternatively spliced variants of the human μ opioid receptor gene, OPRM. Neuroscience 133 (2005) 209-220
22. Pan Y.X., Xu J., Bolan E., Moskowitz H.S., Xu H., and Pasternak G.W. Identification of four novel MOR-1B splice variants of the mouse mu opioid receptor gene: functional consequences of C-Terminus splicing. Mol Pharmacol 68 (2005) 866-875
23. Paxinos G, Watson C. The rat brain in stereotaxic coordinates; 1986.
24. *,8aalpha, 8abeta, 12bbeta)]7,10-Dimethyl-1-methoxy-11-(2-methylpropyl)oxycarbonyl 5,6,7,8,12,12b-hexahydro-(9H)-4,8-methanobenzofuro[3,2-e]pyrrolo[2,3-g]i so quinoline hydrochloride (SB-235863) in blocking hyperalgesia associated with inflammatory and neuropathic pain responses. J Pharmacol Exp Ther 307 (2003) 1079-1089
25. Quock R.M., Burkey T.H., Varga E., Hosohata Y., Hosohata K., Cowell S.M., et al. The delta-opioid receptor: molecular pharmacology, signal transduction, and the determination of drug efficacy. Pharmacol Rev 51 (1999) 503-532
26. Raynor K., Kong H., Chen Y., Yasuda K., Yu L., Bell G.I., et al. Pharmacological characterization of the cloned kappa-, delta-, and mu-opioid receptors. Mol Pharmacol 45 (1994) 330-334
27. Raynor K., Kong H., Mestek A., Bye L.S., Tian M., Liu J., et al. Characterization of the cloned human mu opioid receptor. J Pharmacol Exp Ther 272 (1995) 423-428
28. Rowe B.P., Saylor D.L., Speth R.C., and Absher D.R. Angiotensin-(1-7) binding at angiotensin II receptors in the rat brain. Regul Pept 56 (1995) 139-146
29. Tribollet E., Dreifuss J.J., Charpak G., Dominik W., and Zaganidis N. Localization and quantitation of tritiated compounds in tissue sections with a gaseous detector of beta particles: comparison with film autoradiography. Proc Natl Acad Sci USA 88 (1991) 1466-1468
30. 3H]opioid receptors in rat brain. Brain Res 179 (1979) 255-270
31. Wentland M.P., Duan W., Cohen D.J., and Bidlack J.M. Selective protection and functionalization of morphine: synthesis and opioid receptor by binding properties of 3-amino-3-desoxymorphine derivatives. J Med Chem 43 (2000) 3558-3565