Glucoamylase isoform (GAII) purified from a thermophilic fungus Scytalidium thermophilum 15.8 with biotechnological potential

African Journal of Biotechnology

Volumn 5, Issue 12, 2006, Pages 1239-1245

  Cereia, Mariana ^a   Guimarães, Luis Henrique S ^a   Peixoto Nogueira, Simone C ^a   Jorge, João A ^a   Terenzi, Héctor F ^a   Greene, Lewis J ^a   Polizeli, Maria De Lourdes T M ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Amylase; Fungus; Glucoamylase; Scytalidium thermophilum; Starch hydrolysis

Indexed keywords

AMMONIA; BARIUM ION; CARBOHYDRATE; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOAMYLASE 2; MAGNESIUM ION; MALTOSE; MANGANESE; RESIN; SODIUM CHLORIDE; SODIUM ION; STARCH; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME PURIFICATION; FUNGAL STRAIN; ION EXCHANGE CHROMATOGRAPHY; KINETICS; MOLECULAR WEIGHT; NONHUMAN; PH MEASUREMENT; TEMPERATURE MEASUREMENT; THERMOSTABILITY;

FUNGI; HUMICOLA GRISEA VAR. THERMOIDEA; SCYTALIDIUM THERMOPHILUM;

EID: 33745230126     PISSN: 16845315     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Aiyer PV (2005). Amylases and their applications. Afr. J. Biotechnol. 4(13): 1525-1529.
2. Aquino ACMM, Jorge JA, Terenzi HF, Polizeli MLTM (2001). Thermostable glucose-tolerant glucoamylase produced by thermophilic fungus Scytalidium thermophilum. Folia Microbiol. 46(1): 11-16.
3. Bergmeyer HV, Bernt E (1974). In: Methods of Enzymatic Analysis, HU Bergmeyer (eds). Verlag-Chimie Academic Press, New York vol. (3): 1205-1215.
4. Blum H, Beier H, Gross HJ (1987). Improved silver staining of plant protein, RNA and DNA in polyacrylamide gels. Electrophoresis. 81: 93-99.
5. Campos L, Félix RC (1995). Purification and characterization of glucoamylase from Humicola grisea. Appl. Environm. Microbiol. 61(6): 2436-2438.
6. Cereia M, Terenzi HF, Jorge JA, Greene LJ, Rosa JC, Polizeli MLTM (2000). Glucoamylase activity from the thermophilic fungus Scytalidium thermophilum. Biochemical and regulatory properties. J. Basic Microbiol. 40(2): 83-92.
7. Chen J, Li D, Zhang Y, Zhou Q (2005). Purification and characterization of a thermostable glucoamylase from Chaetomium thermophilum. J. Gen. Appl. Microbiol. 51: 175-181.
8. Chun SB, Bai S, Im SY, Choi WK, Lee JJ (1995). Purification and characterization of two extracellular glucoamylase isoenzymes from Lipomyces kononenkoae CBS 5608 mutant. J. Biochem. Mol. Biol. 28(5): 375-381.
9. Coutinho PM, Reilly PJ (1997). Glucoamylase, structural, functional and evolutionary relationships. Proteins 29: 334-347.
10. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356.
11. Fagerström R, Vainio A, Suoranta K, Pakula T, Kalkkinen N, Torkkeli H (1990). Comparison of two glucoamylases from Hormoconis resinae. J. Gen. Microbiol. 136: 913-920.
12. Fontana JD, Gebara M, Blumel M, Schineider H, Mackenzie CR, Fohnson KG (1988) α-4-O-methyl-D-glucoronidase component of xylanolytic complexes. Methods Enzymol. 160: 560-571.
13. Hames BD, Jurd RD (1985). Gel electrophoresis of proteins: a practical approach. IRL Press, Oxford, Chapter 7. Washington D.C.
14. Horváthová V, Janecek S, Sturdík E (2000). Amylolytic enzymes: their specifities, origins and properties. Biologia (Brastilava) 55(6): 605-615.
15. Iwashita K, Todoroki K, Kimura H, Shimoi H, Ito K. (1998). Purification and characterization of extracellular and cell wall bound β-glucosidases from Aspergillus kawachii. Biosci. Biotechnol. Biochem. 62 (10): 1938-1946.
16. James JA, Lee BH (1997). Glucoamylases: microbial sources, industrial applications and molecular biology - a review. J. Food Biochem. 21: 1-52.
17. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.
18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 267-275.
19. MacKenzie DA, Jeenes DJ, Gou X, Archer DB (2000). Molecular basis of glucoamylase overproduction by a mutagenised industrial strain of Aspergillus niger. Enz. Microbiol. Technol. 26: 193-200.
20. Nguyen QD, Rezessy-Szabó JM, Claeysssens M, Stals I, Hoschke A (2002). Purification and characterization of amylolytic enzymes from thermophilic fungus Thermomyces lanuginosus strain ATCC 34626. Enz. Microbial. Technol. 31: 345-352.
21. O'Farrel PZ, Goodman HM, O'Farrel PH (1977). High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133-1142.
22. Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R (2000). Advances in microbial amylases. Biotechnol. Appl. Biochem. 31: 135-152.
23. Pazur JH, Liu B, Miskiel FJ (1990). Comparison of the properties of glucoamylases of Rhizopus niveus and Aspergillus niger. Biotechnol. Appl. Biochem. 12: 63-78.
24. Reisfield RA, Sewis VJ, Willians DC (1962). Disc electrophoresis of basis proteins and peptides on polyacrylamide gels. Nature 195: 281-283.
25. Straatsma G, Samson RA (1993). Taxonomy of Scytalidium thermophilum, an important fungus in mushroom compost. Mycol. Res. 97(3): 321-328.
26. Taylor PM, Napier EJ, Fleming LD (1978). Some properties of glucoamylase produced by the thermophilic fungus Humicola lanuginosa. Carbohyd. Res. 16: 301-308.
27. Tosi LRO, Terenzi HF, Jorge JA (1993). Purification and characterization of an extracellular glucoamylase from the thermophilic fungus Humicola grisea var. thermoidea. Can. J. Microbiol. 39: 846-852.
28. Towbin H, Staehlin T, Gordon J (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and applications. Proc. Natl. Acad. Sci. USA 76(9): 4350-4354.
29. Wilson M, Nakane P (1978). In: Immunofluorescence and related staining techniques. Elsevier North Holland Biomedical Press, Amsterdam. p. 215.