Hybridase activity of human ribonuclease-1 revealed by a real-time fluorometric assay

Nucleic Acids Research

Volumn 34, Issue 10, 2006, Pages 2906-2913

  Potenza, Nicoletta ^a   Salvatore, Vincenzo ^a   Migliozzi, Annalucia ^a   Martone, Valentina ^a   Nobile, Valentina ^a   Russo, Aniello ^a  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PANCREATIC RIBONUCLEASE; RIBONUCLEASE A; RIBONUCLEASE H; RNA;

ACCURACY; ARTICLE; CONTROLLED STUDY; CYTOSOL; DNA RNA HYBRIDIZATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; FLUOROMETRY; HUMAN; IONIC STRENGTH; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RNA DEGRADATION; RNA HYBRIDIZATION; SENSITIVITY ANALYSIS; SEQUENCE HOMOLOGY; ENZYME SPECIFICITY; ENZYMOLOGY; KINETICS; METABOLISM; OSMOLARITY; SPECTROFLUOROMETRY;

BOS TAURUS; ESCHERICHIA COLI;

DNA; ESCHERICHIA COLI; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; OSMOLAR CONCENTRATION; RIBONUCLEASE H, CALF THYMUS; RIBONUCLEASE, PANCREATIC; RNA; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

EID: 33745150448     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkl368     Document Type: Article

References (39)

1. Weickmann, J.L., Elson, M. and Glitz, D.G. (1981) Purification and characterization of human pancreatic ribonuclease. Biochemistry, 20, 1272-1278.
2. Beintema, J.J., Wietzes, P., Weickmann, J.L. and Glitz, D.G. (1984) The amino acid sequence of human pancreatic ribonuclease. Anal. Biochem., 136, 48-64.
3. Beintema, J.J. and Kleinedam, R.G. (1998) The ribonuclease A superfamily: General discussion. Cell. Mol. Life Sci., 54, 825-832.
4. Cho, S., Beintema, J.J. and Zhang, J. (2005) The ribonuclease A superfamily of mammals and birds: Identifying new members and tracing evolutionary histories. Genomics, 85, 208-220.
5. Blackburn, P. and Moore, S. (1982) Pancreatic ribonucleases. In Boyer, P.D. (ed.), The Enzymes, 3rd edn. Academic Press, Inc., NY, Vol. 15, pp. 317-433.
6. Raines, R.T. (1998) Ribonuclease A. Chem. Rev., 98, 1045-1065.
7. Ribó, M., Benito, A., Canals, A., Nogués, M.V., Cuchillo, C.M. and Vilanova, M. (2001) Purification of engineered human pancreatic ribonuclease. Methods Enzymol., 341, 221-234.
8. Libonati, M. and Sorrentino, S. (2001) Degradation of double-stranded RNA by mammalian pancreatic-type ribonucleases. Methods Enzymol., 341, 234-248.
9. Sorrentino, S., Naddeo, M., Russo, A. and D'Alessio, G. (2003) Degradation of double-stranded RNA by human pancreatic ribonuclease: crucial role of noncatalytic basic amino acid residues. Biochemistry, 42, 10182-10190.
10. Futami, J., Tsushima, Y., Murato, Y., Tada, H., Sasaki, J., Seno, M. and Yamada, H. (1997) Tissue-specific expression of pancreatic-type RNases and RNase inhibitor in humans. DNA Cell Biol., 16, 413-419.
11. Landré, J.B.P., Hewett, P.W., Olivot, J., Friendi, P., Ko, Y., Sachinidis, A. and Moenner, M. (2002) Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1). J. Cell. Biochem., 86, 540-552.
12. Sasso, M.P., Lombardi, M., Confalone, E., Carsana, A., Palmieri, M. and Furia, A. (1999) The differential pattern of tissue-specific expression of ruminant pancreatic type ribonucleases may help to understand the evolutionary history of their genes. Gene, 227, 205-212.
13. Weickmann, J.L. and Glitz, D.G. (1982) Human ribonucleases. Quantitation of pancreatic-like enzymes in serum, urine, and organ preparations. J. Biol. Chem., 257, 8705-8710.
14. Morita, T., Niwata, Y., Ohgi, K., Ogawa, M. and Irie, M. (1986) Distribution of two urinary ribonuclease-like enzymes in human organs and body fluids. J. Biochem., 99, 17-25.
15. Sorrentino, S., De Prisco, R. and Libonati, M. (1989) Human seminal ribonuclease. Immunological quantitation of cross-reactive enzymes in serum, urine and seminal plasma. Biochim. Biophys. Acta, 998, 97-101.
16. Beintema, J.J. (1990) The primary structure of langur (Presbytis entellus) pancreatic ribonuclease: Adaptive features in digestive enzymes in mammals. Mol. Biol. Evol., 7, 470-477.
17. Cullen, B.R. (2002) RNA interference: Antiviral defense and genetic tool. Nature Immunol., 3, 597-599.
18. Barker, R.L., Loegering, D.A., Ten, R.M., Hamann, K.J., Pease, L.R. and Gleich, G.J. (1989) Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases. J. Immunol., 143, 952-955.
19. Lehrer, R.I., Szklarek, D., Barton, A., Ganz, T., Hamann, K.J. and Gleich, G.J. (1989) Antibacterial properties of eosinophil major basic protein and eosinophil cationic protein. J. Immunol., 142, 4428-4434.
20. Rosenberg, H.F. (1995) Recombinant human eosinophil cationic protein. Ribonuclease activity is not essential for cytotoxicity. J. Biol. Chem., 270, 7876-7881.
21. Harder, J. and Schroder, J.M. (2002) RNase 7, a novel innate immune defense antimicrobial protein of healthy human skin. J. Biol. Chem., 277, 46779-46784.
22. Lee-Huang, S., Huang, P.L., Kung, H.F., Blithe, D.L. and Chen, H.C. (1999) Lysozyme and RNases as anti-HIV components in beta-core preparations of human chorionic gonadotropin. Proc. Natl Acad. Sci. USA, 96, 2678-2681.
23. Stein, H. and Hausen, P. (1969) Enzyme from calf thymus degrading the RNA moiety of DNA-RNA hybrids: Effect on DNA-dependent RNA polymerase. Science, 166, 393-395.
24. Crouch, R.J. (2005) Ribonuclease H: From discovery to 3D structure. New Biol., 2, 771-777.
25. Sela, M. and Anfisen, C.B. (1957) Some spectrophotometric and polarimetric experiments with ribonuclease. Biochim. Biophys. Acta, 24, 229-235.
26. Russo, N., Antignani, A. and D'Alessio, G. (2000) In vitro evolution of a dimeric variant of human pancreatic ribonuclease. Biochemistry, 39, 3585-3591.
27. Rizzo, J., Gifford, L.K., Zhang, X., Gewirtz, A.M. and Lu, P. (2002) Chimeric RNA-DNA molecular beacon assay for ribonuclease H activity. Mol. Cell. Probes, 16, 277-283.
28. Kanaya, S., Katsuda, C., Kimura, S., Nakai, T., Kitakuni, E., Nakamura, H., Katayanagi, K., Morikawa, K. and Ikehara, M. (1991) Stabilization of Escherichia coli ribonuclease H. by introduction of an artificial disulfide bond. J. Biol. Chem., 266, 6038-6044.
29. Kanaya, S. (2001) Prokaryotic type 2 RNases H. Methods Enzymol., 341, 377-395.
30. Parniak, M.A., Min, K.L., Budihas, S.R., Le Grice, S.F. and Beutler, J.A. (2003) A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity. Anal. Biochem., 322, 33-39.
31. Nowotny, M., Gaidamakov, S.A., Crouch, R.J. and Yang, W. (2005) Crystal structures of RNase H bound to an RNA/DNA hybrid: Substrate specificity and metal-dependent catalysis. Cell, 121, 1005-1016.
32. Shapiro, R., Fett, J.F., Strydom, DJ. and Vallee, B.L. (1986) Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity. Biochemistry, 25, 7255-7264.
33. Sorrentino, S., Lavitrano, M., De Prisco, R. and Libonati, M. (1985) Human seminal ribonuclease. A tool to check the role of basic charges and glycosylation of a ribonuclease in the action of the enzyme on double-stranded RNA. Biochim. Biophys. Acta, 827, 135-139.
34. Ovesen, L., Bendtsen, F., Tage-Jensen, U., Pedersen, N.T., Gram, B.R. and Rune, S.J. (1986) Intraluminal pH in the stomach, duodenum, and proximal jejunum in normal subjects and patients with exocrine pancreatic insufficiency. Gastroenterology, 90, 958-962.
35. Lee, F.S. and Vallee, B.L. (1993) Structure and action of mammalian ribonuclease (angiogenin) inhibitor. Prog. Nucleic Acid Res. Mol. Biol., 44, 1-30.
36. Boix, E., Wu, Y., Vasandani, V.M., Saxena, S.K. Ardelt, W., Ladner, J. and Youle, R.J. (1996) Role of the N terminus in RNase A homologues: differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity. J. Mol. Biol., 257, 992-1007.
37. Schein, C.H. (1997) From housekeeper to microsurgeon: The diagnostic and therapeutic potential of ribonucleases. Nat. Biotechnol., 15, 529-536.
38. Naskalski, J.W., Kusnierz-Cabala, B., Panek, J. and Kedra, B. (2003) Poly-C specific ribonuclease activity correlates with increased concentrations of IL-6, IL-8 and sTNFR55/sTNFR75 in plasma of patients with acute pancreatitis. J. Physiol. Pharmacol., 54. 439-448.
39. Peracaula, R., Royle, L., Tabares, G., Mallorqui-Fernandez, G., Barrabes, S, Harvey, D.J., Dwek, R.A., Rudd, P.M. and de Llorens, R. (2003) Glycosylation of human pancreatic ribonuclease: Differences between normal and tumor states. Glycobiology, 13, 227-244.