Characterization of human DHRS6, an orphan short chain dehydrogenase/ reductase enzyme: A novel, cytosolic type 2 R-β-hydroxybutyrate dehydrogenase

Journal of Biological Chemistry

Volumn 281, Issue 15, 2006, Pages 10291-10297

  Guo, Kunde ^a   Lukacik, Petra ^a   Papagrigoriou, Evangelos ^a   Meier, Marc ^b   Wen, Hwa Lee ^a   Adamski, Jerzy ^b   Oppermann, Udo ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; ENZYME KINETICS; GENES; GENETIC ENGINEERING; KETONES;

GENE ANALYSIS; HUMAN DHRS6; HYDROXYBUTYRATE DEHYDROGENASES; SHORT CHAIN DEHYDROGENASES;

ENZYMES;

2 HYDROXYBUTYRATE DEHYDROGENASE; ARGININE; DJRS 6 ENZYME; GREEN FLUORESCENT PROTEIN; OXIDOREDUCTASE; UNCLASSIFIED DRUG; DHRS6 PROTEIN, HUMAN; HYDROXYBUTYRATE DEHYDROGENASE; LIPID; SULFATE;

AMINO ACID SEQUENCE; ARTICLE; COMPLEX FORMATION; CRYSTAL STRUCTURE; CYTOSOL; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; GENETIC TRANSFECTION; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN LOCALIZATION; REPORTER GENE; SEQUENCE HOMOLOGY; ANIMAL; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DOSE RESPONSE; ENZYME SPECIFICITY; ENZYMOLOGY; EXON; GENETICS; HELA CELL; HUMAN; KINETICS; METABOLISM; MITOCHONDRION; MOLECULAR CLONING; MOLECULAR GENETICS; PH; PHYLOGENY; PROTEIN CONFORMATION; PROTEIN MOTIF; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); MAMMALIA;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ARGININE; BINDING SITES; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; CYTOSOL; DOSE-RESPONSE RELATIONSHIP, DRUG; EXONS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROXYBUTYRATE DEHYDROGENASE; KINETICS; LIPIDS; MITOCHONDRIA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PHYLOGENY; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; SULFATES;

EID: 33744541159     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M511346200     Document Type: Article

References (25)

1. Chelius, D., Loeb-Hennard, C., Fleischer, S., McIntyre, J. O., Marks, A. R., De, S., Hahn, S., Jehl, M. M., Moeller, J., Philipp, R., Wise, J. G., and Trommer, W. E. (2000) Biochemistry 39, 9687-9697
2. Churchill, P., Hempel, J., Romovacek, H., Zhang, W. W., Brennan, M., and Churchill, S. (1992) Biochemistry 31, 3793-3799
3. Dalton, L. A., McIntyre, J. O., and Fleischer, S. (1993) Biochem. J. 296, 563-569
4. Green, D., Marks, A. R., Fleischer, S., and McIntyre, J. O. (1996) Biochemistry 35, 8158-8165
5. Marks, A. R., McIntyre, J. O., Duncan, T. M., Erdjument-Bromage, H., Tempst, P., and Fleischer, S. (1992) J. Biol. Chem. 267, 15459-15463
6. Jornvall, H., Persson, B., Krook, M., Atrian, S., Gonzalez-Duarte, R., Jeffery, J., and Ghosh, D. (1995) Biochemistry 34, 6003-6013
7. Kallberg, Y., Oppermann, U., Jornvall, H., and Persson, B. (2002) Protein Sci. 11, 636-641
8. Oppermann, U., Filling, C., Hult, M., Shafqat, N., Wu, X., Lindh, M., Shafqat, J., Nordling, E., Kallberg, Y., Persson, B., and Jornvall, H. (2003) Chem. Biol. Interact. 143, 247-253
9. Oppermann, U. C., Filling, C., and Jornvall, H. (2001) Chem. Biol. Interact. 130, 699-705
10. Totrov, M., and Abagyan, R. (1997) Proteins, Suppl. 1, 215-220
11. Powell, A. J., Read, J. A., Banfield, M. J., Gunn-Moore, F., Yan, S. D., Lustbader, J., Stern, A. R., Stern, D. M., and Brady, R. L. (2000) J. Mol. Biol. 303, 311-327
12. Ghosh, D., Weeks, C. M., Grochulski, P., Duax, W. L., Erman, M., Rimsay, R. L., and Orr, J. C. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 10064-10068
13. Tanaka, N., Nonaka, T., Nakanishi, M., Deyashiki, Y., Hara, A., and Mitsui, Y. (1996) Structure 4, 33-45
14. Ghosh, D., Wawrzak, Z., Weeks, C. M., Duax, W. L., and Erman, M. (1994) Structure 2, 629-640
15. Benach, J., Atrian, S., Gonzalez-Duarte, R., and Ladenstein, R. (1999) J. Mol. Biol. 289, 335-355
16. Filling, C., Berndt, K. D., Benach, J., Knapp, S., Prozorovski, T., Nordling, E., Ladenstein, R., Jornvall, H., and Oppermann, U. (2002) J. Biol. Chem. 277, 25677-25684
17. Oppermann, U. C., Filling, C., Berndt, K. D., Persson, B., Benach, J., Ladenstein, R., and Jornvall, H. (1997) Biochemistry 36, 34-40
18. Hawes, J. W., Crabb, D. W., Chan, R. J., Rougraff, P. M., Paxton, R., and Harris, R. A. (2000) Methods Enzymol. 324, 218-228
19. Hawes, J. W., Harper, E. T., Crabb, D. W., and Harris, R. A. (1997) Adv. Exp. Med. Biol. 414, 395-402
20. Breitkreuz, K. E., Allan, W. L., Van Cauwenberghe, O. R., Jakobs, C., Talibi, D., Andre, B., and Shelp, B. J. (2003) J. Biol. Chem. 278, 41552-41556
21. Aneja, P., and Charles, T. C. (1999) J. Bacteriol. 181, 849-857
22. Endemann, G., Goetz, P. G., Edmond, J., and Brunengraber, H. (1982) J. Biol. Chem. 257, 3434-3440
23. Ohnuki, M., Takahashi, N., Yamasaki, M., and Fukui, T. (2005) Biochim. Biophys. Acta 1729, 147-153
24. Koundakjian, P. P., and Snoswell, A. M. (1970) Biochem. J. 119, 49-57
25. Leblanc, P. J., and Ballantyne, J. S. (2000) J. Exp. Zool. 286, 434-439