Active site directed mutagenesis of 3β/17β-hydroxysteroid dehydrogenase establishes differential effects on short-chain dehydrogenase/reductase reactions

Biochemistry

Volumn 36, Issue 1, 1997, Pages 34-40

  Oppermann, Udo C T ^a   Filling, Charlotta ^a   Berndt, Kurt D ^a   Persson, Bengt ^a   Benach, Jordi ^a   Ladenstein, Rudolf ^a   Jörnvall, Hans ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

17-HYDROXYSTEROID DEHYDROGENASES; BACTERIAL PROTEINS; BINDING SITES; BLOTTING, WESTERN; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GRAM-NEGATIVE AEROBIC RODS AND COCCI; GUANIDINE; GUANIDINES; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NAD; POINT MUTATION; PROTEIN DENATURATION; SOFTWARE;

EID: 0031021249     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi961803v     Document Type: Article

References (35)

1. Abalain, J. H., Di Stefano, S., Amet, Y., Quemener, E., Abalain-Colloc, M. L., & Floch, H. H. (1993) J. Steroid Biochem. Mol. Biol. 44, 133-139.
2. Baker, M. E. (1994) Steroids 59, 248-258.
3. Barski, O. A., Gabbay, K. H., Grimshaw, C. E., & Bohren, K. M. (1995) Biochemistiy 34, 11264-11275.
4. Benach, J., Knapp, S., Oppermann, U. C. T., Hägglund, O., Jörnvall, H., & Ladenstein, R. (1996) Eur. J. Biochem. 236, 144-148.
5. Bohren, K. M., Bullock, B., Wermuth, B., & Gabbay, K. H. (1989) J. Biol. Chem. 264, 9547-9551.
6. Chen, Z., Lin, Z. G., Lee, W. R., & Chang, S. H. (1991) Eur. J. Biochem. 202, 263-267.
7. Chen, Z., Jiang, J. C., Lin, Z. G., Lee, W. R., Baker, M. E., & Chang, S. H. (1993) Biochemistry 32, 3342-3346.
8. Ensor, M. E., & Tai, H. H. (1994) Biochim. Biophys. Acta 1208, 151-156.
9. Ensor, M. E., & Tai, H. H. ( 1996) Biochem. Biophys. Res. Commun. 220, 330-333.
10. Ghosh, D., Weeks, C. M., Grochulski, P., Duax, W. L., Erman, M., Rimsay, R. L., & Orr, J. C. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 10064-10068.
11. Ghosh, D., Wawrzak, Z., Weeks, C. M., Duax, W. L., & Erman, M. (1994) Structure 2, 629-640.
12. Ghosh, D., Pletnev, V. Z., Zhu, D. W., Wawrzak, Z., Duax, W. L., Pangborn, W., Labrie, F., & Lin, S. X. (1995) Structure 3, 503-513.
13. Jörnvall, H., von Bahr-Lindström, H., Jany, K. D., Ulmer, W., & Fröschle, M. (1984) FEBS Lett. 165, 190-196.
14. Jörnvall, H., Persson, B., Krook, M., Atrian, S., Gonzalez-Duarte, R., Jeffery, J., & Ghosh, D. (1995) Biochemistry 34, 6003-6013.
15. Krook, M., Marekov, L., & Jörnvall, H. (1990) Biochemistry 29, 738-743.
16. Krook, M., Prozorowski, V., Atrian, S., Gonzalez-Duarte, R., & Jörnvall, H. (1992) Eur. J. Biochem. 209, 233-239.
17. Krook, M., Ghosh, D., Duax, W., & Jörnvall, H. (1993) FEBS Lett. 322, 139-142.
18. Krozowski, Z. (1994) J. Steroid Biochem. Mol. Biol. 51, 125-130.
19. Kyhse-Andersen, J. ( 1984) J. Biochem. Biophys. Methods 10, 203-209.
20. Laemmli, U. K. (1970) Nature 227, 680-685.
21. Marcus, P. I., & Talalay, P. (1956) J. Biol. Chem. 218, 661-674.
22. Minard, P., Legoy, M.-D., & Thomas, D. (1985) FEBS Lett. 188, 85-90.
23. Nozaki, Y. (1972) Methods Enzymol. 26, 43-51.
24. Obeid, J., & White, P. C. (1992) Biochem. Biophys. Res. Commun. 188, 222-227.
25. Pace, C. N. (1986) Methods Enzymol. 131, 266-280.
26. Persson, B., Krook, M., & Jörnvall, H. (1991) Eur. J. Biochem. 200, 537-543.
27. Rossmann, M. G., Moras, D., & Olsen, K. W. (1974) Nature 250, 194-199.
28. Santoro, M. M., & Bolen, D. W. (1988) Biochemistry 27, 8063-8068.
29. Schultz, R. M., Groman, E. V., & Engel, L. L. (1977) J. Biol. Chem. 252, 3784-3790.
30. Talalay, P., & Marcus, P. I. (1956) J. Biol. Chem. 218, 675-691.
31. Tamaoka, J., Ha, D. M., & Komagata, K. (1987) Int. J. Syst. Bacteriol. 37, 52-59.
32. Tanaka, N., Nonaka, T., Nakanishi, M., Deyashiki, Y., Hara, A., & Mitsui, Y. (1996) Structure 4, 33-45.
33. Taylor, J. W., Ott, J., & Eckstein, F. (1985) Nucleic Acids Res. 13, 8764-8785.
34. Varughese, K. I., Skinner, M. M., Whiteley, J. M., Matthews, D. A., & Xuong, N. H. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 6080-6084.
35. Yin, S. J., Vagelopoulos, N., Lundquist, G., & Jörnvall, H. (1991) Eur. J. Biochem. 197, 359-365.