Accumulation of FlAsH/Lumio Green in active mitochondria can be reversed by β-mercaptoethanol for specific staining of tetracysteine-tagged proteins

Histochemistry and Cell Biology

Volumn 125, Issue 6, 2006, Pages 743-747

  Langhorst, Matthias F ^a   Genisyuerek, Selda ^a   Stuermer, Claudia A O ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Biarsenical fluorescent ligands; Fluorescence microscopy; Live cell imaging; Reggie flotillin; Tetracysteine

Indexed keywords

ARSENIC; CYSTEINE; ETHYLENE GLYCOL; MERCAPTOETHANOL; PROTEIN; THIOL;

ANIMAL CELL; ARTICLE; CELL LABELING; CELL LINE; CELL TYPE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOTOXICITY; FLUORESCENCE MICROSCOPY; HELA CELL; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION SWELLING; NONHUMAN; PRIORITY JOURNAL; RAT; SIGNAL NOISE RATIO; STAINING;

CYSTEINE; FLUORESCEINS; FLUORESCENT DYES; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MERCAPTOETHANOL; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; ORGANOMETALLIC COMPOUNDS; REPETITIVE SEQUENCES, AMINO ACID; STAINING AND LABELING;

ANIMALIA;

EID: 33646872862     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-005-0136-3     Document Type: Article

References (20)

1. Abramoff MD, Magelhaes PJ, Ram SJ (2004) Image processing with image. J Biophotonics Int 11:36-42
2. Andresen M, Schmitz-Salue R, Jakobs S (2004) Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging. Mol Biol Cell 15:5616-5622
3. Chen I, Ting AY (2005) Site-specific labeling of proteins with small molecules in live cells. Curr Opin Biotechnol 16:35-40
4. Dalton WS (2002) Targeting the mitochondria: an exciting new approach to myeloma therapy. Commentary re: Bahlis NJ et al. (2002) Feasibility and correlates of arsenic trioxide combined with ascorbic acid-mediated depletion of intracellular glutathione for the treatment of relapsed/refractory multiple myeloma. Clin Cancer Res 8:3658-3668. Clin Cancer Res 8:3643-3645
5. Gaietta G, Deerinck TJ, Adams SR, Bouwer J, Tour O, Laird DW, Sosinsky GE, Tsien RY, Ellisman MH (2002) Multicolor and electron microscopic imaging of connexin trafficking. Science 296:503-507
6. Griffin BA, Adams SR, Jones J, Tsien RY (2000) Fluorescent labeling of recombinant proteins in living cells with FlAsH. Methods Enzymol 327:565-578
7. Griffin BA, Adams SR, Tsien RY (1998) Specific covalent labeling of recombinant protein molecules inside live cells. Science 281:269-272
8. Lang DM, Lommel S, Jung M, Ankerhold R, Petrausch B, Laessing U, Wiechers MF, Plattner H, Stuermer CA (1998) Identification of reggie-1 and reggie-2 as plasmamembrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons. J Neurobiol 37:502-523
9. Langhorst MF, Reuter A, Stuermer CA (2005) Scaffolding microdomains and beyond: the function of reggie/flotillin proteins. Cell Mol Life Sci 62:2228-2240
10. Lindahl PE, Oberg KE (1960) Mechanism of the physiological action of rotenone. Nature 187:784
11. Lippincott-Schwartz J, Snapp E, Kenworthy A (2001) Studying protein dynamics in living cells. Nat Rev Mol Cell Biol 2:444-456
12. Marek KW, Davis GW (2002) Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I. Neuron 36:805-813
13. Martin BR, Giepmans BN, Adams SR, Tsien RY (2005) Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity. Nat Biotechnol 23(10):1308-1314
14. Neumann-Giesen C, Falkenbach B, Beicht P, Claasen S, Luers G, Stuermer CA, Herzog V, Tikkanen R (2004) Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression. Biochem J 378:509-518
15. Schulte T, Paschke KA, Laessing U, Lottspeich F, Stuermer CA (1997) Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration. Development 124:577-87
16. Song L, Hennink EJ, Young IT, Tanke HJ (1995) Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys J 68:2588-600
17. Stroffekova K, Proenza C, Beam KG (2001) The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins. Pflugers Arch 442:859-66
18. Tour O, Meijer RM, Zacharias DA, Adams SR, Tsien RY (2003) Genetically targeted chromophore-assisted light inactivation. Nat Biotechnol 21:1505-1508
19. Urano Y, Kamiya M, Kanda K, Ueno T, Hirose K, Nagano T (2005) Evolution of fluorescein as a platform for finely tunable fluorescence probes. J Am Chem Soc 127:4888-4894
20. Zmuda J, Friedenson B (1983) Changes in intracellular glutathione levels in stimulated and unstimulated lymphocytes in the presence of 2-mercaptoethanol or cysteine. J Immunol 130:362-364