A coupled spectrophotometric assay for l-cysteine:1-d-myo-inosityl 2-amino-2-deoxy-α-d-glucopyranoside ligase and its application for inhibitor screening

Analytical Biochemistry

Volumn 353, Issue 2, 2006, Pages 167-173

  Newton, Gerald L ^a   Ta, Philong ^a   Sareen, Dipti ^a   Fahey, Robert C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cysteine; Inhibitor screening assay; Ligase; MshC; MshC assay; Mycothiol; Mycothiol biosynthesis

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLYBDENUM COMPOUNDS; PEPTIDES; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY;

CYSTEINE; INHIBITOR SCREENINGS; LIGASE; MSHC; MYCOTHIOL;

AMINO ACIDS;

1 DEXTRO MYO INOSITYL 2 AMINO 3 DEOXY ALPHA DEXTRO GLUCOPYRANOSIDE LIGASE; CYSTEINE; GLUTATHIONE; INORGANIC PYROPHOSPHATASE; LIGASE; MALACHITE GREEN; MYCOTHIOL; PHOSPHATE; PYROPHOSPHATE; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ACTINOBACTERIA; ARTICLE; CLONING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; SCREENING; SPECTROPHOTOMETRY; TUBERCULOSIS;

ACTINOBACTERIA (CLASS); MYCOBACTERIUM TUBERCULOSIS;

EID: 33646826677     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.03.030     Document Type: Article

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